Complexation of bases and phosphates of nucleic acid components by transition metal ions

Methylene blue (MB) is a typical photosensitizing agent and a DNA hybridization indicator, but its modes of interaction with the DNA molecules are not clearly described, particularly in relation to its electrochemical oxidation signals. To probe the DNA-MB interactions we have used chromosomal salmon testes and supercoiled plasmid sc pUC19 DNA immobilized on home-made screen-printed electrodes (SPEs) and a wide range of MB concentrations, from nano-to micromolar. The applicability of the home-made screen-printed electrodes used for the DNA-MB studies were tested using standard calf thymus DNA. Two MB oxidation peak signals: MB(I) at ca. À 0.18 V and MB(II) at 0 V vs. Ag/AgCl were detected within AE 10 -15% standard deviation, signals different from adsorbed MB signal (À 0.25 V, pH 4.7). The MB(I) signal, seen when both DNAs were used, showed two plateaus, one at nano-and another at micromolar MB concentrations; these were accompanied by the changes in the oxidation signal at 0.98 V, usually attributed to guanine oxidation. In contrast, the MB(II) signal was only seen for salmon testes DNA, indicating various modes of MB interactions with chromosomal and plasmid DNA. In the presence of MB, the guanine related signal (G) at 0.98 V has been amplified significantly (10 Â ), allowing for the identification of the DNAs at low DNA concentrations, the feature particularly useful in the plasmid sc pUC19 detection. The use of another DNA intercalator, riboflavin (RF), aided in the identification of the relation between MB(I), MB(II) and G oxidation signals.

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“…Similarly, when metal ions, such as Ni(II), complex DNA [48] or its components [49], their reduction peaks shift positively. [43].…”

Section: Mb Interactions With Salmon Testes and Plasmid Puc19 Dna Immmentioning

confidence: 99%

Methylene Blue Interactions with Chromosomal and Plasmid DNA on Screen‐Printed Carbon Electrodes

Palinska¹,

Grodzka²,

Elżanowska³

et al. 2010

Electroanalysis

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“…Complexation of base residues involved in the DNA hydrogen bonding system by Ni(II) species was detected in our previous work [5]. In [4] and [5], complexation between Ni(II) and base residues was inferred from the electrocatalytic reduction of Ni(II) yielded by polynucleotides, DNA and their purine base components.…”

Section: Introductionmentioning

confidence: 95%

“…These compounds induce the B to Z conformational transition of some polynucleotides [3]. The nature of this transition has been explained as a change in stacking interactions between base residues due to their complexation by Ni(II) ion [4]. Complexation of base residues involved in the DNA hydrogen bonding system by Ni(II) species was detected in our previous work [5].…”

Section: Introductionmentioning

confidence: 99%

Determination of Stability Constants for Complexes of Ni(II) with Adenine and Guanine from Catalytic Linear Scan Voltammetric Currents

2002

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The catalytic effects of adenine and guanine on the reduction of Ni(II) at the hanging mercury drop electrode were used to study the formation of complexes in the bulk of aqueous solutions at 25 °C and ionic strength 0.2 M. Complexation data were obtained from an analysis of the kinetic (catalytic) pure limiting currents, according to a method proposed previously. Values of 3.70 and 3.17 were determined for the logarithms of the stability constants corresponding to the formation of 1 : 1 complexes between Ni(II) – aquo ion and the neutral form of adenine and guanine, respectively.

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“…The presence of Zn-Tris (trishydroxylaminomethane) complex in neutral aqueous solutions influences the polarographic behavior of enzymes which need Zn 2 + ions for developing its activity [58]. The splitting of Ni(II) polarographic wave in the presence of bases and phosphate residues of nucleic acid components [59] was attributed to the reduction of free NF+ ions and Ni(II)-base complexes. The stability constants of these complexes may be arranged into the series guanin > 5-methylcytosine > adenine > cytosine > 5-methyluracil.…”

Section: Further Trends and Selected Applicationsmentioning

confidence: 99%

“…The subject of further polarographic studies was the B-Z helical transition of two types of polynucleotides [ 60]. The nature of this transition was explained as a change in stacking interactions between bases of nucleic acids due to their complexation by the Ni(II) and Mn(II) ions.…”

Section: Further Trends and Selected Applicationsmentioning

confidence: 99%

Basic principles of voltammetry

Fischer

Fischerová

1995

Experimental Techniques in Bioelectrochemistry

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Complexation of bases and phosphates of nucleic acid components by transition metal ions

Cited by 16 publications

References 44 publications

Methylene Blue Interactions with Chromosomal and Plasmid DNA on Screen‐Printed Carbon Electrodes

Methylene Blue Interactions with Chromosomal and Plasmid DNA on Screen‐Printed Carbon Electrodes

Determination of Stability Constants for Complexes of Ni(II) with Adenine and Guanine from Catalytic Linear Scan Voltammetric Currents

Basic principles of voltammetry

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