Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy

Zhang, Daoyong; Pan, Xiangliang; Mostofa, Khan M. G.; Chen, Xi; Mu, Guijin; Wu, Fengchang; Liu, Jing; Song, Wenjing; Yang, Jianying; Li, Yanli; Fu, Qing‐Long

doi:10.1016/j.jhazmat.2009.10.011

Cited by 150 publications

(59 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the hydrolyzate of Al 3+ at acid or alkaline conditions may have weak fluorescence quenching effects on the different functional groups of humus-like substances. The same trend was also observed in previous research on the complexation between mercury and humic substances (Chai et al, 2012;Zhang et al, 2010).…”

Section: Eps Fluorescence Quenching By Al 3+supporting

confidence: 89%

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Ruan

Liu

2013

Journal of Environmental Sciences

View full text Add to dashboard Cite

Aluminum flocculant can enhance the flocculating performance of activated sludge. However, the binding mechanism of aluminum ion (Al 3+ ) and extracellular polymeric substances (EPS) in activated sludge is unclear due to the complexity of EPS. In this work, threedimensional excitation emission matrix fluorescence spectroscopy (3DEEM), fluorescence quenching titration and Fourier transform infrared spectroscopy (FT-IR) were used to explore the binding behavior and mechanism between Al 3+ and EPS. The results showed that two fluorescence peaks of tyrosine-and tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances (LB-EPS), and three peaks of tyrosine-, tryptophan-and humic-like substances were identified in the tightly boundextracellular polymeric substances (TB-EPS). It was found that these fluorescence peaks could be quenched with Al 3+ at the dosage of 3.0 mg/L, which demonstrated that strong interactions took place between the EPS and Al 3+ . The conditional stability constants for Al 3+ and EPS were determined by the Stern-Volmer equation. As to the binding mechanism, the -OH, N-H, C=O, C-N groups and the sulfur-and phosphorus-containing groups showed complexation action, although the groups in the LB-EPS and TB-EPS showed different behavior. The TB-EPS have stronger binding ability to Al 3+ than the LB-EPS, and TB-EPS play an important role in the interaction with Al 3+ .

show abstract

Section: Eps Fluorescence Quenching By Al 3+supporting

confidence: 89%

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Ruan

Liu

2013

Journal of Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…In this study it was utilized for quantitating the functional groups involved in the binding of Cd(II) with root exudates. The fluorescence excitation emission matrix (EEM) spectrometry is a simple and sensitive modern technique that has been widely used to provide quantitative information on the interaction between fluorescent organic matter and metal ions (Wu et al 2001;Yamashita & Tanoue 2003;Jaffe´et al 2004;Zhang et al 2010). Since proteins are key components of root exudates with fluorescent properties, in the present study, the fluorescent components as protein in root exudates and their interaction with Cd were investigated by EEM fluorescence spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Root exudates from sunflower (Helianthus annuusL.) show a strong adsorption ability toward Cd(II)

Yang

Pan

2013

Journal of Plant Interactions

Self Cite

View full text Add to dashboard Cite

Cd(II) adsorption of root exudates from sunflower (Helianthus annuus L.) seedling was investigated by Cd ionselective electrode, Fourier Transform Infrared spectroscopy, and fluorescence spectroscopy. Root exudates from Helianthus annuus L. had strong adsorption ability toward Cd(II). The adsorption process was pH-dependent and the maximum adsorption capacity, 150.8 mg g(1 , was observed at pH 7.0. Root exudates had pK a1 at 4.7 for carboxyl and pK a2 at 9.2 for phenolic, and amino groups. The aliphatic and aromatic (C (H) groups, amide III group, and the C ( 0O) (O and sulfonate groups were responsible for Cd(II) adsorption. The excitation emission matrix fluorescence spectroscopy showed protein-like substances participated in Cd adsorption and formed strong complexes, with conditional stability constants of 4.70 and 4.32, which is a little lower than that determined by potentiometric methods, 5.13. The strong Cd complexing ability of root exudates implies that root exudates may significantly affect mobility, toxicity, and phytoavailability of Cd. Cd binding of root exudates may be attributed to its interaction with the proteins, polysaccharides, and phenolic compounds in root exudates.

show abstract

“…It was reported that EPS accounted for over 90% of organic substances in the biofilm. [33] Therefore, EPS contents generally reflected the biomass of the biofilm. In this study, the protein contents in the diatom-biofilm of N. closterium and culture mediums of the two diatoms could not be detected, probably because of the low concentrations.…”

Section: Discussionmentioning

confidence: 99%

The influence of bacterial quorum-sensing inhibitors against the formation of the diatom-biofilm

Yang

Song

Zhu

et al. 2016

Chemistry and Ecology

View full text Add to dashboard Cite

Quorum-sensing inhibitor (QSI) is one of the most promising and environmentally friendly agents for marine antifouling. In this study, the activities of three kinds of QSIs 3,4-dibromo-2(5)Hfuranone, 4-nitropyridine-N-oxide and indole were evaluated on the growth of two marine diatoms Cylinthrotheca sp. and Nitzschia closterium. At the same time, the effects of QSIs on the formation of the diatom-biofilm were also discussed. All the results showed that QSIs significantly inhibited the growth, and the effects depended on the dose and diatom species. The extracellular polymeric substance contents in the diatom-biofilm were significantly reduced by QSIs. However, the contents of polysaccharide in culture mediums were increased, which might result in the destruction of diatom cells. Combined with the results of crystal violet staining-biofilm and images of scanning electron microscopy, it was further demonstrated that QSIs inhibited the biofilm formation of Cylindrotheca sp., and the inhibitory effect of 4-nitropyridine-N-oxide was superior to that of 3,4-dibromo-2(5)H-furanone and indole.ARTICLE HISTORY

show abstract

Complexation between Hg(II) and biofilm extracellular polymeric substances: An application of fluorescence spectroscopy

Cited by 150 publications

References 50 publications

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Root exudates from sunflower (Helianthus annuusL.) show a strong adsorption ability toward Cd(II)

The influence of bacterial quorum-sensing inhibitors against the formation of the diatom-biofilm

Contact Info

Product

Resources

About