Complex Transcriptional Units: Diversity in Gene Expression by Alternative Rna Processing

Leff, Stuart E.; Rosenfeld, Michael G.; Evans, Ronald M.

doi:10.1146/annurev.bi.55.070186.005303

Cited by 487 publications

(147 citation statements)

References 114 publications

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“…This contrasts with the "usual" situation in mammals, the genes of which possess single or widely spaced (alternatively utilized) poly (A) sites (Leff et al, 1986;Wahle, 1995). The presence of multiple polyadenylation sites in plant genes implies the presence of multiple corresponding cis elements, a suggestion that has been confirmed in a number of instances.…”

Section: Polyadenylation Sicnals In Plant Nuclear Mrna Precursorsmentioning

confidence: 81%

The Polyadenylation of RNA in Plants

Li¹,

Hunt²

1997

Plant Physiology

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Section: Polyadenylation Sicnals In Plant Nuclear Mrna Precursorsmentioning

confidence: 81%

The Polyadenylation of RNA in Plants

Li¹,

Hunt²

1997

Plant Physiology

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“…Splicing of conventional pre-mRNA has been shown to depend on the precise identification of splice sites and on the orderly joining of each and every exon (reviewed by Aebi and Weissmann, 1987). In alternative splicing, the precision is maintained, but the pre-mRNA can be spliced in more than one way, thus allowing a gene to encode multiple protein isoforms (reviewed by Leff et al, 1986;. Many instances of alternative splicing are stochastic and not obviously regulated.…”

Section: Introductionmentioning

confidence: 99%

Regulation of tissue-specific alternative splicing: exon-specific cis-elements govern the splicing of leukocyte common antigen pre-mRNA.

1989

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“…This process occurs by a variety of mechanisms and results in the exclusion of exon sequences and/or retention of intervening sequences (WSs) in the spliced mRNA (Left et al 1986;Breitbart et al 1987}. In addition to increasing the diversity of proteins produced from a limited DNA sequence, another important consequence of alternative splicing is the regulation, in some cases, of mRNA levels in response to developmental or tissuespecific signals.…”

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confidence: 99%

A Sequential splicing mechanism promotes selection of an optimal exon by repositioning a downstream 5' splice site in preprotachykinin pre-mRNA.

Nasim¹,

Spears²,

Hoffmann³

et al. 1990

Genes Dev.

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To explore the structural basis of alternative splicing, we have analyzed the splicing of pre-mRNAs containing an optional exon, E4, from the preprotachykinin gene. This gene encodes substance P and related tachykinin peptides by alternative splicing of a common pre-mRNA. We have shown that alternative splicing of preprotachykinin pre-mRNA occurs by preferential skipping of optional E4. The competing mechanism that incorporates E4 into the final spliced RNA is constrained by an initial block to splicing of the immediate upstream intervening sequence (IVS), IVS3. This block is relieved by sequential splicing, in which the immediate downstream IVS4 is removed first. The structural change resulting from the first splicing event is directly responsible for activation of IVS3 splicing. This structural rearrangement replaces IVS4 sequences with E5 and its adjacent IVS5 sequences. To determine how this structural change promoted IVS3 splicing, we asked what structural change(s) would restore activity of IVS3 splicing-defective mutants. The most significant effect was observed by a 2-nucleotide substitution that convened the 5' splice site of E4 to an exact consensus match, GUAAGU. Exon 5 sequences alone were found not to promote splicing when present in one or multiple copies. However, when a 15-nucleotide segment of IVS5 containing GUAAGU was inserted into a splicing-defective mutant just downstream of the hybrid exon segment E4E5, splicing activity was recovered. Curiously, the 72-nucleotide L2 exon of adenovirus, without its associated 5' splice site, activates splicing when juxtaposed to E4. Models for the activation of splicing by an RNA structural change are discussed.

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Complex Transcriptional Units: Diversity in Gene Expression by Alternative Rna Processing

Cited by 487 publications

References 114 publications

The Polyadenylation of RNA in Plants

The Polyadenylation of RNA in Plants

Regulation of tissue-specific alternative splicing: exon-specific cis-elements govern the splicing of leukocyte common antigen pre-mRNA.

A Sequential splicing mechanism promotes selection of an optimal exon by repositioning a downstream 5' splice site in preprotachykinin pre-mRNA.

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