Complex RNA processing of TDRKH, a novel gene encoding the putative RNA-binding Tudor and KH domains

Readers of histone methylarginine marks

“…TDRKH, also referred to as TDRD2, is the only Tudor domain-containing protein to also harbor KH domains, which bind RNA [82]. Initial RNA-based expression studies showed that TDRKH is present in a number of different tissues (high in brain and heart) [82], and Western analysis identified expression in the testis and brain [38].…”

“…Initial RNA-based expression studies showed that TDRKH is present in a number of different tissues (high in brain and heart) [82], and Western analysis identified expression in the testis and brain [38]. The mouse knockout model of TDRKH revealed that it is essential for spermatogenesis and piRNA biogenesis in the germline [83].…”

Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi

“…The Tdrkh protein contains one Tudor domain and 2 tandem KH domains and has only been described in terms of gene cloning and mRNA expression (20). Since the tissue distribution and subcellular localization of the Tdrkh protein has not been studied, we used affinity purified anti-Tdrkh antibody to examine these points (Fig.…”

Histone Recognition

“…TDRKH, also referred to as TDRD2, is the only Tudor domain-containing protein to also harbor KH domains, which bind RNA (Lamb et al 2000). The mouse knockout model of TDRKH revealed that it is essential for spermatogenesis and piRNA biogenesis in the germline (Saxe et al 2013).…”

“…The mouse knockout model of TDRKH revealed that it is essential for spermatogenesis and piRNA biogenesis in the germline (Saxe et al 2013). However, expression data from the The Human Protein Atlas reveal that TDRKH is not restricted to germ cells, and both RNA-based studies (Lamb et al 2000) and Western analysis ) confirm high expression in the testis, heart, and brain. Thus, TDRKH may have additional non-germ cell functions.…”