Complex representation of taste quality by second-order gustatory neurons in Drosophila

Snell, Nathaniel J.; Fisher, John D.; Hartmann, G. C.; Zolyomi, Bence; Talay, Mustafa; Barnea, Gilad

doi:10.1016/j.cub.2022.07.048

Cited by 24 publications

(18 citation statements)

References 51 publications

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“…We observed many local neurons projecting within the SEZ as well as three major projections to the superior protocerebrum: a lateral, medial, and mediolateral tract ( Figure 6A and Figure 6—figure supplement 1 ). This pattern is consistent with second-order bitter neurons labeled in other recent studies ( Chen et al, 2019 ; Snell et al, 2022 ). Thus, at the first synapse, the bitter taste circuit diverges into at least three downstream pathways projecting out of the SEZ.…”

Section: Resultssupporting

confidence: 92%

“…Through transsynaptic tracing, we identified three major second-order pathways that relay bitter input to higher brain areas, which have also been described in other recent studies ( Chen et al, 2019 ; Snell et al, 2022 ). Each pathway receives input from multiple organs, although our experiments do not confirm that different inputs converge onto the same individual cells.…”

Section: Discussionsupporting

confidence: 64%

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Selective integration of diverse taste inputs within a single taste modality

Deere

Sarkissian

Yang

et al. 2023

eLife

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A fundamental question in sensory processing is how different channels of sensory input are processed to regulate behavior. Different input channels may converge onto common downstream pathways to drive the same behaviors, or they may activate separate pathways to regulate distinct behaviors. We investigated this question in the Drosophila bitter taste system, which contains diverse bitter-sensing cells residing in different taste organs. First, we optogenetically activated subsets of bitter neurons within each organ. These subsets elicited broad and highly overlapping behavioral effects, suggesting that they converge onto common downstream pathways, but we also observed behavioral differences that argue for biased convergence. Consistent with these results, transsynaptic tracing revealed that bitter neurons in different organs connect to overlapping downstream pathways with biased connectivity. We investigated taste processing in one type of downstream bitter neuron that projects to the higher brain. These neurons integrate input from multiple organs and regulate specific taste-related behaviors. We then traced downstream circuits, providing the first glimpse into taste processing in the higher brain. Together, these results reveal that different bitter inputs are selectively integrated early in the circuit, enabling the pooling of information, while the circuit then diverges into multiple pathways that may have different roles.

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Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 64%

Selective integration of diverse taste inputs within a single taste modality

Deere

Sarkissian

Yang

et al. 2023

eLife

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“…In retro -Tango, this design provides genetic access to the presynaptic neurons. Therefore, the reporter can be readily swapped with an effector that allows for monitoring (Snell et al, 2022), activation, or inhibition of the presynaptic neurons. In addition, the modular design facilitates the adaptation of retro -Tango to other organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Flies were reared on standard cornmeal/agar/molasses media. Fly lines used in this study are: GF-split-Gal4 (von Reyn et al, 2014); Or67d Gal4 (Kurtovic et al, 2007); ss00090-Gal4 (Wolff & Rubin, 2018); SAG-split-Gal4 (ss51118) (Wang et al, 2021); aDN-split-Gal4 (Nojima et al, 2021); QUAS-nls-DsRed (Snell et al, 2022); QUAS-mtdTomato(3xHA) (this study); retro- Tango(panneuronal) (this study); retro- Tango(ligand) (this study).…”

Section: Methodsmentioning

confidence: 99%

retro-Tango enables versatile retrograde circuit tracing inDrosophila

Sorkaç

Moșneanu

Crown

et al. 2022

Preprint

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Transsynaptic tracing methods are crucial tools in studying neural circuits. Although a couple of anterograde tracing methods and a targeted retrograde tool have been developed inDrosophila melanogaster, there is still need for an unbiased, user-friendly, and flexible retrograde tracing system. Here we describeretro-Tango, a method for transsynaptic, retrograde circuit tracing and manipulation inDrosophila. In this genetically encoded system, a ligand-receptor interaction at the synapse triggers an intracellular signaling cascade that results in reporter gene expression in presynaptic neurons. Importantly, panneuronal expression of the elements of the cascade renders this method versatile, enabling its use not only to test hypotheses but also to generate them. We validateretro-Tango in various circuits and benchmark it by comparing our findings with the electron microscopy reconstruction of theDrosophilahemibrain. Our experiments establishretro-Tango as a key method for circuit tracing in neuroscience research.

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“…This division of labor to modulate a single behavioral output, PER, may shed light on why the processing of a single taste involves multiple parallel pathways. Recent anatomical studies have revealed that pathways for individual taste modalities diverge into several branches at the first synapse ( Talay et al., 2017 ; Deere et al., 2022 ; Snell et al., 2022 ). We have proposed that these parallel pathways may regulate different behaviors ( Deere et al., 2022 ), but the present results also suggest that they may modulate the same behavior, PER, in different contexts.…”

Section: Discussionmentioning

confidence: 99%