Complex Regulation by Apetala2 Domain-Containing Transcription Factors Revealed through Analysis of the Stress-Responsive TdCor410b Promoter from Durum Wheat

Eini, Omid; Yang, Nannan; Pyvovarenko, Tatiana; Pillman, Katherine A.; Bazanova, Natalia; Tikhomirov, Natalia; Eliby, Serik; Shirley, Neil J.; Sivasankar, S.; Tingey, Scott V.; Langridge, Peter; Hrmová, Mária; Lopato, Sergiy

doi:10.1371/journal.pone.0058713

Cited by 31 publications

(33 citation statements)

References 86 publications

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“…It is known that ERFs specifically bind to the GCC‐box and sometimes to CRT DNA cis ‐elements, whereas DREB TFs specifically recognize DRE and CRT sequences (Eini et al, ). The affinities of the GCC‐Box, DRE, and CRT cis ‐elements to TaSHN1 were determined using an Y1H assay (Figure c).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Shi

Kovalchuk

et al. 2018

Plant Cell & Environment

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Transcription factors regulate multiple networks, mediating the responses of organisms to stresses, including drought. Here, we investigated the role of the wheat transcription factor TaSHN1 in crop growth and drought tolerance. TaSHN1, isolated from bread wheat, was characterized for molecular interactions and functionality. The overexpression of TaSHN1 in wheat was followed by the evaluation of T and T transgenic lines for drought tolerance, growth, and yield components. Leaf surface changes were analysed by light microscopy, SEM, TEM, and GC-MS/GC-FID. TaSHN1 behaves as a transcriptional activator in a yeast transactivation assay and binds stress-related DNA cis-elements, determinants of which were revealed using 3D molecular modelling. The overexpression of TaSHN1 in transgenic wheat did not result in a yield penalty under the controlled plant growth conditions of a glasshouse. Transgenic lines had significantly lower stomatal density and leaf water loss and exhibited improved recovery after severe drought, compared with control plants. The comparative analysis of cuticular waxes revealed an increased accumulation of alkanes in leaves of transgenic lines. Our data demonstrate that TaSHN1 may operate as a positive modulator of drought stress tolerance. Positive attributes could be mediated through an enhanced accumulation of alkanes and reduced stomatal density.

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Section: Resultsmentioning

confidence: 99%

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Shi

Kovalchuk

et al. 2018

Plant Cell & Environment

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“…With the aim to decrease differences in the developmental phenotypes of control and transgenic wheat plants, a stress‐inducible promoter of barley, Dehydrin 8 ( Dhn8 ), was also used for overexpression of TaRAP2.1L . The structure and properties of the Dnh8 promoter (James et al ., ; Xiao and Xue, ) are similar to those of the TdCor410b promoter (Eini et al ., ), showing strong induction by cold, drought and salinity, a moderate level of constitutive expression in leaves and a high level of expression in the developing grain. A total of 12 independent transgenic lines of wheat (cv.…”

Section: Resultsmentioning

confidence: 99%

“…Members of the ERF subfamily differ from DREBs/CBFs in their recognition of specific cis ‐elements (Canella et al ., ; Eini et al ., ; Hao et al ., ; Sakuma et al ., ). Most studied ERFs recognize and serve promoters containing GCC‐box elements (AGCCGCC), although in some cases, they can either additionally or solely bind to CRT elements (GCCGAC) (Eini et al ., ). While the mechanisms for positive regulation of downstream stress‐related genes by ERFs and DREB transcription factors (TFs) are reasonably well studied, mechanisms of negative regulation by the members of the DREB and ERF subfamilies, containing repressor motifs, are less well understood.…”

Section: Introductionmentioning

confidence: 99%

Change of function of the wheat stress‐responsive transcriptional repressor TaRAP2.1L by repressor motif modification

Amalraj

Luang

Kumar

et al. 2015

Plant Biotechnology Journal

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Summary Plants respond to abiotic stresses by changes in gene regulation, including stress‐inducible expression of transcriptional activators and repressors. One of the best characterized families of drought‐related transcription factors are dehydration‐responsive element binding (DREB) proteins, known as C‐repeat binding factors (CBF). The wheat DREB/CBF gene TaRAP2.1L was isolated from drought‐affected tissues using a dehydration‐responsive element (DRE) as bait in a yeast one‐hybrid screen. TaRAP2.1L is induced by elevated abscisic acid, drought and cold. A C‐terminal ethylene responsive factor‐associated amphiphilic repression (EAR) motif, known to be responsible for active repression of target genes, was identified in the TaRAP2.1L protein. It was found that TaRAP2.1L has a unique selectivity of DNA‐binding, which differs from that of DREB activators. This binding selectivity remains unchanged in a TaRAP2.1L variant with an inactivated EAR motif (TaRAP2.1Lmut). To study the role of the TaRAP2.1L repressor activity associated with the EAR motif in planta, transgenic wheat overexpressing native or mutated TaRAP2.1L was generated. Overexpression of TaRAP2.1L under constitutive and stress‐inducible promoters in transgenic wheat and barley led to dwarfism and decreased frost tolerance. By contrast, constitutive overexpression of the TaRAP2.1Lmut gene had little or no negative influence on wheat development or grain yield. Transgenic lines with the TaRAP2.1Lmut transgene had an enhanced ability to survive frost and drought. The improved stress tolerance is attributed to up‐regulation of several stress‐related genes known to be downstream genes of DREB/CBF activators.

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“…The generation of vectors for plant transformation has been described previously (Eini et al ., ; Kovalchuk et al ., ; Morran et al ., ). Briefly, the 2x35S promoter was excised from the pMDC32 vector (Curtis and Grossniklaus, ) and replaced with one of three promoters: the constitutive ZmUbiquitin promoter (Eini et al ., ), or stress‐inducible OsWRKY71 or TdCor39 promoters (Kovalchuk et al ., ), resulting in pUbi, pWRKY71 and pCor39 vectors, respectively. A 767‐bp fragment of the TaHDZipI‐5 coding sequence (CDS) (GenBank accession ) (Harris et al ., ) was isolated from T. aestivum L. cv.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of the class I homeodomain transcription factor TaHDZipI‐5 increases drought and frost tolerance in transgenic wheat

Yang

Luang

Harris

et al. 2017

Plant Biotechnology Journal

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SummaryCharacterization of the function of stress‐related genes helps to understand the mechanisms of plant responses to environmental conditions. The findings of this work defined the role of the wheat TaHDZipI‐5 gene, encoding a stress‐responsive homeodomain–leucine zipper class I (HD‐Zip I) transcription factor, during the development of plant tolerance to frost and drought. Strong induction of TaHDZipI‐5 expression by low temperatures, and the elevated TaHDZipI‐5 levels of expression in flowers and early developing grains in the absence of stress, suggests that TaHDZipI‐5 is involved in the regulation of frost tolerance at flowering. The TaHDZipI‐5 protein behaved as an activator in a yeast transactivation assay, and the TaHDZipI‐5 activation domain was localized to its C‐terminus. The TaHDZipI‐5 protein homo‐ and hetero‐dimerizes with related TaHDZipI‐3, and differences between DNA interactions in both dimers were specified at 3D molecular levels. The constitutive overexpression of TaHDZipI‐5 in bread wheat significantly enhanced frost and drought tolerance of transgenic wheat lines with the appearance of undesired phenotypic features, which included a reduced plant size and biomass, delayed flowering and a grain yield decrease. An attempt to improve the phenotype of transgenic wheat by the application of stress‐inducible promoters with contrasting properties did not lead to the elimination of undesired phenotype, apparently due to strict spatial requirements for TaHDZipI‐5 overexpression.

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Complex Regulation by Apetala2 Domain-Containing Transcription Factors Revealed through Analysis of the Stress-Responsive TdCor410b Promoter from Durum Wheat

Cited by 31 publications

References 86 publications

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions

Change of function of the wheat stress‐responsive transcriptional repressor TaRAP2.1L by repressor motif modification

Overexpression of the class I homeodomain transcription factor TaHDZipI‐5 increases drought and frost tolerance in transgenic wheat

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