Complex interactions of in-stream dissolved organic matter and nutrient spiralling unravelled by Bayesian regression analysis

Abstract

Abstract. Uptake and release patterns of dissolved organic matter (DOM) compounds and co-transported nutrients are entangled, and the current literature does not provide a consistent picture of the interactions between the retention processes of DOM fractions. We performed plateau addition experiments with five different complex DOM leachates in a small experimental stream impacted by diffuse agricultural pollution. The study used a wide range of DOM qualities by including leachates of cow dung, pig dung, corn leaves, leaves from trees, and whole nettle plants. We measured changes in nutrient and dissolved organic carbon (DOC) concentrations along the stream course and determined DOM fractions by fluorescence measurements and parallel factor (PARAFAC) decomposition. To assess the influences of hydrological transport processes, we used a 1D hydrodynamic model. We developed a non-linear Bayesian approach based on the nutrient spiralling concept, which we named the “interactions in nutrient spirals using Bayesian regression” (INSBIRE) approach. This approach can disentangle complex interactions of biotic and abiotic drivers of reactive solutes' uptake in multi-component DOM sources. It can show the variability of the uptake velocities and quantify their uncertainty distributions. Furthermore, previous knowledge of nutrient spiralling can be included in the model using prior probability distributions. We used INSBIRE to assess interactions of compound-specific DOM and nutrient spiralling metrics in our experiment. Bulk DOC uptake varied among sources, showing decreasing uptake velocities in the following order: corn > pig dung > leaves > nettles > cow dung. We found no correlations between bulk DOC uptake and the amounts of protein-like compounds or co-leached soluble reactive phosphorus (SRP). The fastest uptake was observed for SRP and the tryptophan-like component, while the other DOM components' uptake velocities more or less resembled that of the bulk DOC. Almost all DOM components showed a negative relationship between uptake and concentration, known as efficiency loss. Furthermore, we observed a few negative and (weak) positive interactions between the uptake and the concentration of different components, such as a decreased uptake of protein-like compounds at high concentrations of a high-molecular-weight humic-like compound. We also found an influence of the wetted width on the uptake of SRP and a microbially derived humic substance, which indicates the importance of the sediment–water interface for P and humic C cycling in the studied stream. Overall, we show that bulk DOC is a weak predictor of DOC uptake behaviour for complex DOM leachates. Individual DOM compound uptake, including co-leached nutrients, is controlled by both internal (quality-related) and external (environmental) factors within the same aquatic ecosystem. We conclude that the cycling of different C fractions and their mutual interaction with N and P uptake in streams is a complex, non-linear problem, which can only be assessed with advanced non-linear approaches, such as the presented INSBIRE approach.

Experimental evaluation of the role of inorganic phosphorus for terrestrial carbon degradation in stream hyporheic zones

“…Moreover, the stability of soil aggregates, as well as the amount and molecular size of the DOC leached from aggregates depended on the ionic strength and Ca 2+ concentration of the water used to extract the DOC. Finally, during the 48–72 hr of soil leaching in the bottle before filtration and preparation for the incubations for our studies, a small part of the labile DOM soil already could have been degraded during the preparation (also reported in Pucher et al, 2021).…”

“…Microbial DOM processing in streams and rivers controls the rates of fluvial carbon retention and carbon dioxide (CO 2 ) outgassing, and alters the quantity and quality of the organic matter transported downstream (Boulton et al, 1998). Despite a growing number of studies on the microbial mineralisation and degradation of DOM in running waters (e.g., Fasching et al, 2014; Pucher et al, 2021), there are still large uncertainties regarding the mechanisms and factors determining the rates of fluvial DOM processing by microbes.…”

Advances in Catchment Science, Hydrochemistry, and Aquatic Ecology Enabled by High-Frequency Water Quality Measurements

“…High-frequency measurements provide information about concentrations of nutrients (total P [TP], reactive P, total N, NO 3 -N) and C (DOC, total organic carbon [TOC]) but also in-depth information on DOM quality and its biogeochemical interactions, e.g., the complexation of DOM with metals such as mercury. 122 For example, Wilson et al 55 used humic-like fluorescence (peak C intensity) as an indicator of biodegradable DOC and dissolved organic nitrogen concentrations. Bulk DOC concentrations alone cannot describe highly variable organic matter composition, but absorbance measurements at specific wavelengths and their ratios can provide additional information on DOM quality.…”