Complex Identifcation of Red Yeast Isolate from Gastrointestinal Tract of Hucul Long-Liver (Carpathians, Ukraine)

Fomina, Marina; Polishchuk, L. V.; Tkachenko, Konstantin S.; Hong, Jonathan; Zelena, Liubov; Ianieva, O.D.; Pidgorskyi, V.S.

doi:10.15407/microbiolj78.05.002

Cited by 2 publications

(2 citation statements)

References 14 publications

(5 reference statements)

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“…In order to detail the taxonomic position of the fungal culture MF18_10, we analyzed the nucleotide sequence of the isolate's ITS-fragment containing the 5'-end of the 18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2, the 3'-end of the 26S rRNA gene [10][11][12]. Universal fungi primers ITS1 5'-TCCGTAGGTGAACCTGCGG-3' and ITS4 5'-TCCTCCGCTTATTGATATGC-3' were used to amplify the ITS chromosome fragment [10].…”

Section: Methodsmentioning

confidence: 99%

“…Universal fungi primers ITS1 5'-TCCGTAGGTGAACCTGCGG-3' and ITS4 5'-TCCTCCGCTTATTGATATGC-3' were used to amplify the ITS chromosome fragment [10]. The nucleotide sequence of the ITS of the isolate MF18_10 (585 bp) was placed in GenBank under the accession number MK367422 and its primary structure was used in our study as a reference sequence [10][11][12].…”

Section: Methodsmentioning

confidence: 99%

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Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Lutsenko¹,

Polishchuk²,

Hong³

et al. 2019

Fakt. eksp. evol. org.

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Aim. The aim of this work is to determine the taxonomic position of the fungal strain MF18_10 isolated from the damaged wall with medieval painting of St. Sophia’s Cathedral (Kyiv, Ukraine). Methods. The fungus was isolated on selective medium for xerophilic fungi (70% sucrose Czapek agar). Мacro- and micro-morphological phenotypic characterization was carried out using light and scanning electron microscopy. Molecular-biological identification was performed using nucleotide sequences of the ITS-fragment. Results. On the basis of phenotypical characteristics of the fungal isolate MF18_10, it was established its affiliation to the genus Penicillium. ITS analysis revealed that the isolate belongs to the species P. chrysogenum demonstrating 100% identity with other 78 P. chrysogenum strains in GenBank database including the type strains NR_077145 and AY373902, also sharing several distinct differences in substitutions, deletions and insertions within this group. Conclusions. The isolated xerotolerant fungus was identified as P. chrysogenum, the typical representative of the indoor environments and dust, and also common for mycobiota of damaged historic-cultural artifacts. The differences in the analyzed P. chrysogenum ITS primary structures did not correlate with the source of isolation. Keywords: Penicillium chrysogenum, xero-tolerant fungi, ITS, scanning electron microscopy.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Lutsenko¹,

Polishchuk²,

Hong³

et al. 2019

Fakt. eksp. evol. org.

Self Cite

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Characteristic of ITS fragment of rDNA of Rhodotorula mucilaginosa isolated from gastrointestinal tract of centenarian

Polishchuk¹,

Tkachenko²,

Fomyna³

1970

Fakt. eksp. evol. org.

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Aim. The aim of the work was to analyse the primary structure of the ITS region of the ribosomal operon of the red yeast isolate Rhodotorula mucilaginosa S1 from the gastrointestinal tract of healthy centenarian compared to the available homological basidimycetous nucleotide sequences. Methods. In silico comparison of the ITS nucleotide sequences using NCBI server resources (BLAST programs and "Nucleotide" database) with the reference ITS-fragment of red yeast isolate was performed. Results. Almost 90 % of highly homologous to the reference ITS-fragment out of 800 selected sequences represented genera Rhodotorula and Rhodosporidium with the top hits for R. mucilaginosa strains PMM08-1716L, 3942L and 3684L. The most frequent nucleobases mismatches of ITS-fragments of GenBank’s R. mucilaginosa strains compared to the reference one were observed in the positions 541 (T/C) for 85 % of strains, and 129 (T/C) and 158 (G/A) for 26 % of strains. Conclusions. The primary structure of studied ITS region showed 100 % homology to R. mucilaginosa strains also isolated from humans and animals, as well as the most frequently mismatches for 129th, 158th and 541st nucleotides located in the spacers regions of the ribosomal operon. Keywords: ITS, rDNA, nucleotide sequence, red yeast, human GIT.

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Complex Identifcation of Red Yeast Isolate from Gastrointestinal Tract of Hucul Long-Liver (Carpathians, Ukraine)

Cited by 2 publications

References 14 publications

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

Characteristic of ITS fragment of rDNA of Rhodotorula mucilaginosa isolated from gastrointestinal tract of centenarian

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