Complex gene regulation between young and old soybean leaves in responses to manganese toxicity

Liu, Ying; Xue, Yingbin; Xie, Baoxing; Zhu, Shengnan; Lu, Xiuyun; Liang, Chaojie; Tian, Jichun

doi:10.1016/j.plaphy.2020.07.002

Cited by 30 publications

(49 citation statements)

References 71 publications

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“…In this study, excess Mn treatments led to increase in Mn levels in soybean, which was accompanied by increased number of brown spots and decreased chlorophyll concentrations, ultimately reducing plant fresh weight of the 14-day-old soybean seedlings ( Figure 1 ). Similar results have been found in previous studies where soybean growth was inhibited by Mn toxicity ( Chen Z. et al, 2016 ; Santos et al, 2017 ; Liu et al, 2020 ), although different growth stages of soybean were applied. Furthermore, important roles of antioxidant systems, such as antioxidant enzymes and non-enzymatic components, have been suggested in the response of soybean to Mn toxicity ( Chen Z. et al, 2016 ; Santos et al, 2017 ; Liu et al, 2020 ).…”

Section: Discussionsupporting

confidence: 90%

“…Similar results have been found in previous studies where soybean growth was inhibited by Mn toxicity ( Chen Z. et al, 2016 ; Santos et al, 2017 ; Liu et al, 2020 ), although different growth stages of soybean were applied. Furthermore, important roles of antioxidant systems, such as antioxidant enzymes and non-enzymatic components, have been suggested in the response of soybean to Mn toxicity ( Chen Z. et al, 2016 ; Santos et al, 2017 ; Liu et al, 2020 ). Although some advances have been made in investigating the response of soybean to Mn toxicity, its potential molecular mechanisms involved in Mn tolerance have not been elucidated.…”

Section: Discussionsupporting

confidence: 90%

“…Soybean ( Glycine max ) is recognized as one of the economically important nitrogen fixing legume crops and provides more than 60% of high-quality protein and oil for humans and animals in the world ( Herridge et al, 2008 ; Lu et al, 2017 ). Although several studies have been conducted to investigate the response of soybean to Mn toxicity ( Lavres et al, 2009 ; Yang et al, 2009 ; Chen Z. et al, 2016 ; Santos et al, 2017 ; Liu et al, 2020 ), underlying molecular mechanisms, through the involvement of MTPs, have not been elucidated. Key participants within the specific Mn-CDF cluster involved in Mn detoxification in soybean remain undetermined.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean

Dong

Jia

et al. 2021

Front. Plant Sci.

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Manganese is an essential micronutrient for plant growth but can be toxic to plants when it reaches excessive levels. Although metal tolerance proteins (MTPs), which belong to the cation diffusion facilitator (CDF) family, have been demonstrated to play critical roles in manganese (Mn) tolerance in plants, the characteristics and functions of GmMTP members in the response of soybean (Glycine max) to Mn toxicity have not been documented. In this study, growth inhibition was observed in soybean plants that were exposed to a toxic level of Mn in hydroponics, as reflected by the generation of brown spots, and decreased leaf chlorophyll concentration and plant fresh weight. Subsequent genome-wide analysis resulted in the identification of a total of 14 GmMTP genes in the soybean genome. Among these GmMTPs, 9 and 12 were found to be regulated by excess Mn in leaves and roots, respectively. Furthermore, the function of GmMTP8.1, a Mn-CDF homologue of ShMTP8 identified in the legume Stylosanthes hamata that is involved in Mn detoxification, was characterized. Subcellular localization analysis showed that GmMTP8.1 was localized to the endoplasmic reticulum (ER). Heterologous expression of GmMTP8.1 led to the restoration of growth of the Mn-hypersensitive yeast (Saccharomyces cerevisiae) mutant Δpmr1, which is made defective in Mn transport into the Golgi apparatus by P-type Ca/Mn-ATPase. Furthermore, GmMTP8.1 overexpression conferred tolerance to the toxic level of Mn in Arabidopsis (Arabidopsis thaliana). Under excess Mn conditions, concentrations of Mn in shoots but not roots were decreased in transgenic Arabidopsis, overexpressing GmMTP8.1 compared to the wild type. The overexpression of GmMTP8.1 also led to the upregulation of several transporter genes responsible for Mn efflux and sequestration in Arabidopsis, such as AtMTP8/11. Taken together, these results suggest that GmMTP8.1 is an ER-localized Mn transporter contributing to confer Mn tolerance by stimulating the export of Mn out of leaf cells and increasing the sequestration of Mn into intracellular compartments.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean

Dong

Jia

et al. 2021

Front. Plant Sci.

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“…The plant material used in this study was soybean with genotype YC03-3. After seeds germination, soybean seedlings were cultured by hydroponics using soybean nutrient solution with two different Mn concentration treatments, 5 (control) or 100 (Mn toxicity) µmol/L MnSO 4 •H 2 O as described before [27]. After 15 days of treatment, the soybean leaves were harvested and frozen in liquid nitrogen for further analysis.…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Transcriptome analysis of soybean leaves response to manganese toxicity

Xue

Chen

et al. 2021

Biotechnology & Biotechnological Equipment

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As an essential micronutrient, manganese (Mn) participates in diverse processes during plant growth and development. Excess accumulation of Mn in plants is toxic, and soybean (Glycine max) growth and production are severely limited by Mn toxicity. However, the molecular basis in adaptation to Mn toxicity for soybean remains largely unknown. In this study, RNA-seq analysis on soybean leaves was conducted, more than 44 million reads were generated, and a total of 38,022 expressed genes were identified. Compared to control, 2258 differentially expressed genes (DEGs), including 744 up-regulated and 1514 down-regulated ones, were obtained. Cellular process, cell part and binding function were the most enriched terms by GO analysis. Furthermore, 49 DEGs were identified in plant hormone signal transduction pathways by KEGG analysis. Among them, Mn toxicity up-regulated AHK, PIF, JAZ and TGA family DEGs might play important roles in the adaptation of soybean leaves to Mn toxicity.

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“…The hydroponic nutrient solutions containing 0, 150, 300, 600 and 900 μmol/L MnSO 4 were prepared according to the reported methods [11], respectively.…”

Section: Preparation Of Nutrient Solutionsmentioning

confidence: 99%

Effects of manganese on growth and development of peanut (Arachis hypogaea L.) seedlings

Liu

Chen

et al. 2021

E3S Web Conf.

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The effects of different menganses (Mn) concentrations on the growth and development of peanut seedlings were analysed in the present study. The results showed that the main root length, lateral root number, plant height, dry weight of aboveground and underground parts of peanut seedlings were all influenced by the concentrations of Mn obviously. Moreover, the growth of peanut could be significantly promoted at low concentration of Mn (150 μmol/L), while inhibited by high concentration of Mn (900 μmol/L). Therefore, these conclusions could help us to understand the most suitable concentration range of Mn for the growth of peanut, and could also analyze the specific performance of the harm of Mn toxicity on peanut, so as to lay a theoretical foundation for improving the yield of peanut and expanding the cultivation area.

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Complex gene regulation between young and old soybean leaves in responses to manganese toxicity

Cited by 30 publications

References 71 publications

Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean

Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean

Transcriptome analysis of soybean leaves response to manganese toxicity

Effects of manganese on growth and development of peanut (Arachis hypogaea L.) seedlings

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