Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction

Single molecule FRET detection in CdSe-QD donor and Cy5-labeled molecular chaperone acceptor complex by imaging microscopy

“…Our protocol for the conjugation in Hepes buffer solution of pH 7.5 with or without potassium chloride (HK) is in Ref. [3], however just briefly, notable points are as follows. The QDs dislike any trace amount of waters primarily in every step of synthesis to its preservation.…”

“…It thus seems possible that they can also capture the QDs due to its surface similarity. Such complex of the QDs and Cy5-labeled molecular chaperones can actually be prepared [3] and their photophysical investigations are ongoing. Among them, detection of resonant energy transfer (FRET) from the donor QDs to the acceptor Cy5-labeled proteins [4] is significant and also a most direct evidence for complex formation.…”

“…Butanol is adopted as a water-soluble catalytic solvent to dissolve the QDs into aqueous solution in order to avoid severe aggregations as possible [3]. While the complex formation of QDs with molecular chaperones in aqueous buffer solution is a hard task, we can provide observable amount of hybrid complex successfully for the single molecule imaging technique [5].…”

Energy transfer in hybrid CdSe quantum dots vs. labelled molecular chaperone systems by imaging microscopy

“…Details of the procedures are described in Ref. [11]. For the moment we have not yet obtained sufficient amount of the conjugates accessible with ordinary biochemical measurements, thus the single molecule imaging technique is significant.…”

“…Samples used for single-molecule observation are prepared as follows: The 10 µL of final solution (QD concentration: 1.6 nM, PFD concentration: 8 nM, see Ref. [11]) is dropped and expanded on the surface of silica glass substrate (25 × 55 × 1 mm…”