Complex Fluids Based on Methacrylated Hyaluronic Acid

Prata, Joseph E.; Barth, Tiffany A.; Bencherif, Sidi A.; Washburn, Newell R.

doi:10.1021/bm901373x

Cited by 45 publications

(28 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also similar to the viscosity behavior observed with methacrylated HA near gels and microgels [27], as well as HA-containing materials in the body, including synovial fluid and the vitreous [28, 29]. The ability to shear thin may be particularly important for comfort on the corneal surface, allowing the gel to thin during a blink.…”

Section: Discussionsupporting

confidence: 59%

A Crosslinked HA-Based Hydrogel Ameliorates Dry Eye Symptoms in Dogs

Williams

Mann

2013

International Journal of Biomaterials

View full text Add to dashboard Cite

Keratoconjunctivitis sicca, commonly referred to as dry eye or KCS, can affect both humans and dogs. The standard of care in treating KCS typically includes daily administration of eye drops to either stimulate tear production or to hydrate and lubricate the corneal surface. Lubricating eye drops are often applied four to six times daily for the life of the patient. In order to reduce this dosing regimen yet still provides sufficient hydration and lubrication, we have developed a crosslinked hydrogel based on a modified, thiolated hyaluronic acid (HA), xCMHA-S. This xCMHA-S gel was found to have different viscosity and rheologic behavior than solutions of noncrosslinked HA. The gel was also able to increase tear breakup time in rabbits, indicating a stabilization of the tear film. Further, in a preliminary clinical study of dogs with KCS, the gel significantly reduced the symptoms associated with KCS within two weeks while only being applied twice daily. The reduction of symptoms combined with the low dosing regimen indicates that this gel may lead to both improved patient health and owner compliance in applying the treatment.

show abstract

Section: Discussionsupporting

confidence: 59%

A Crosslinked HA-Based Hydrogel Ameliorates Dry Eye Symptoms in Dogs

Williams

Mann

2013

International Journal of Biomaterials

View full text Add to dashboard Cite

show abstract

“…In a surgical context, this translates to allowing appropriate shaping and contouring to the defect site of interest. Yield stresses of up to 62 Pa have been previously reported for HA-based solutions, 18 but this yield stress may not be sufficient for topical application. In the current study, we demonstrated the ability to obtain solutions with yield stresses over 700 Pa. For context, the yield stresses for common paste-like materials, such as toothpaste, are approximately 200 Pa. Because the only solutions exhibiting a yield stress were solutions that incorporated HAnp, the yield stress was attributed to the HA being in the nanoparticulate form, as the addition of HA that was the same MW but was linear instead of in nanoparticle form was insufficient for achieving a yield stress.…”

Section: Discussionmentioning

confidence: 95%

Enabling Surgical Placement of Hydrogels Through Achieving Paste-Like Rheological Behavior in Hydrogel Precursor Solutions

et al. 2015

View full text Add to dashboard Cite

Hydrogels are a promising class of materials for tissue regeneration, but they lack the ability to be molded into a defect site by a surgeon because hydrogel precursors are liquid solutions that are prone to leaking during placement. Therefore, although the main focus of hydrogel technology and developments are on hydrogels in their crosslinked form, our primary focus is on improving the fluid behavior of hydrogel precursor solutions. In this work, we introduce a method to achieve paste-like hydrogel precursor solutions by combining hyaluronic acid nanoparticles with traditional crosslinked hyaluronic acid hydrogels. Prior to crosslinking, the samples underwent rheological testing to assess yield stress and recovery using linear hyaluronic acid as a control. The experimental groups containing nanoparticles were the only solutions that exhibited a yield stress, demonstrating that the nanoparticulate rather than the linear form of hyaluronic acid was necessary to achieve paste-like behavior. The gels were also photocrosslinked and further characterized as solids, where it was demonstrated that the inclusion of nanoparticles did not adversely affect the compressive modulus and that encapsulated bone marrow-derived mesenchymal stem cells remained viable. Overall, this nanoparticle-based approach provides a platform hydrogel system that exhibits a yield stress prior to crosslinking, and can then be crosslinked into a hydrogel that is capable of encapsulating cells that remain viable. This behavior may hold significant impact for hydrogel applications where a paste-like behavior is desired in the hydrogel precursor solution.

show abstract

“…HAGM was prepared by grafting methacrylate groups to hyaluronic acid [32,35,66]. A total of 1 gram of hyaluronic acid (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 200 mL phosphate buffered saline (PBS, Sigma-Aldrich, pH 7.4) and subsequently mixed with 67 mL of dimethylformamide (Sigma-Aldrich), 13.3 g of glycidyl methacrylate (Sigma-Aldrich), and 6.7 g of triethylamine (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications

et al. 2018

View full text Add to dashboard Cite

Polymeric scaffolds such as hydrogels can be engineered to restore, maintain, or improve impaired tissues and organs. However, most hydrogels require surgical implantation that can cause several complications such as infection and damage to adjacent tissues. Therefore, developing minimally invasive strategies is of critical importance for these purposes. Herein, we developed several injectable cryogels made out of hyaluronic acid and gelatin for tissue-engineering applications. The physicochemical properties of hyaluronic acid combined with the intrinsic cell-adhesion properties of gelatin can provide suitable physical support for the attachment, survival, and spreading of cells. The physical characteristics of pure gelatin cryogels, such as mechanics and injectability, were enhanced once copolymerized with hyaluronic acid. Reciprocally, the adhesion of 3T3 cells cultured in hyaluronic acid cryogels was enhanced when formulated with gelatin. Furthermore, cryogels had a minimal effect on bone marrow dendritic cell activation, suggesting their cytocompatibility. Finally, in vitro studies revealed that copolymerizing gelatin with hyaluronic acid did not significantly alter their respective intrinsic biological properties. These findings suggest that hyaluronic acid-co-gelatin cryogels combined the favorable inherent properties of each biopolymer, providing a mechanically robust, cell-responsive, macroporous, and injectable platform for tissue-engineering applications.

show abstract

Complex Fluids Based on Methacrylated Hyaluronic Acid

Cited by 45 publications

References 34 publications

A Crosslinked HA-Based Hydrogel Ameliorates Dry Eye Symptoms in Dogs

A Crosslinked HA-Based Hydrogel Ameliorates Dry Eye Symptoms in Dogs

Enabling Surgical Placement of Hydrogels Through Achieving Paste-Like Rheological Behavior in Hydrogel Precursor Solutions

Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications

Contact Info

Product

Resources

About