Completion of the sexual cycle and demonstration of genetic recombination in Ustilago maydis in vitro

Ruiz-Herrera, Jos&#x000E9;; León-Ramírez, Claudia Geraldine; Cabrera-Ponce, José Luis; Martínez-Espinoza, Alfredo D.; Herrera-Estrella, Luis

doi:10.1007/s004380051107

Cited by 29 publications

(15 citation statements)

References 14 publications

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“…Ustilago maydis requires contact with the host, maize, to complete its sexual life cycle. Recently, it was demonstrated that small molecules from maize tissue could substitute for the intact tissue (35). In the obligate biotrophic fungus, Gigaspora gigantea, and related species, presymbiotic hyphal growth and branching was induced by an active factor present in root exudates of host, but not nonhost, plants (36).…”

Section: Discussionmentioning

confidence: 99%

Homoserine and asparagine are host signals that trigger in planta expression of a pathogenesis gene inNectria haematococca

Yang

Rogers

Song

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Some pathogenesis-related genes are expressed in fungi only when the pathogen is in the host, but the host signals that trigger these gene expressions have not been identified. Virulent Nectria haematococca infects pea plants and requires either pelA, which is induced by pectin, or pelD, which is induced only in planta. However, the host signal(s) that trigger pelD expression was unknown. Here we report the isolation of the host signals and identify homoserine and asparagine, two free amino acids found in uniquely high levels in pea seedlings, as the pelD-inducing signals. N. haematococca has evolved a mechanism to sense the host tissue environment by using the high levels of two free amino acids in this plant, thereby triggering the expression of pelD to assist the pathogenic process.fungal infection ͉ pectate lyase ͉ Pisum sativum ͉ host-pathogen interactions ͉ pathogen-host coevolution

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Section: Discussionmentioning

confidence: 99%

Homoserine and asparagine are host signals that trigger in planta expression of a pathogenesis gene inNectria haematococca

Yang

Rogers

Song

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Completion of the sexual cycle and spore production occurs under normal circumstances only in the infected plant. A long-standing goal of Ustilago researchers has been reached quite recently, when Ruiz-Herrera et al (1999) reported the successful production of sexual spores in fungal mycelium grown in close contact with embryogenic maize cell cultures in vitro. This will open a new window to follow the molecular details of fungal development during plant infection.…”

Section: Figmentioning

confidence: 99%

Ustilago maydis – a valuable model system for the study of fungal dimorphism and virulence

Bölker

2001

Microbiology

146

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“…Teliospores undergo meiosis to produce the haploid phase (reviewed in Christensen 1963, Banuett 2002, Klosterman et al 2007. Because the developmental program leading to teliospore formation does not occur outside the plant it has been hypothesized that plant signals trigger this pathway (Banuett and Herskowitz 1996, Regenfelder et al 1997, Ruiz-Herrera et al 1999.…”

Section: Introductionmentioning

confidence: 99%

Fuz1, a MYND domain protein, is required for cell morphogenesis inUstilago maydis

Chew

Aweiss²,

Lu³

et al. 2008

Mycologia

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Ustilago maydis is a Basidiomycete fungus that exhibits a yeast-like nonpathogenic form and a dikaryotic filamentous pathogenic form. Generation of these two forms is controlled by two mating type loci, a and b. The fungus undergoes additional morphological transitions in the plant that result in formation of a third cell type, the teliospore. The fuz1 gene is necessary for this developmental program. Here we report cloning and sequencing of fuz1 and show that it contains an open reading frame with coding capacity for a protein of 1421 amino acids. The Fuz1 protein belongs to the family of MYND Zn finger domain proteins. We generate a null mutation in strains of opposite mating type and show that fuz1 is necessary for conjugation tube formation, a morphological transition that occurs in response to pheromones. We generate fuz1 − diploid strains heterozygous at a and b and show that fuz1 is also necessary for postfusion events (maintenance of filamentous growth). We also demonstrate that fuz1 is necessary for cell morphogenesis of the yeast-like cell: normal cell length, location and number of septa, cell separation and constriction of the neck region. Fuz1 is also required for cell wall integrity and to prevent secretion of a dark pigment. We propose that the MYND domain may interact with different proteins to regulate cell morphogenesis.

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Completion of the sexual cycle and demonstration of genetic recombination in Ustilago maydis in vitro

Cited by 29 publications

References 14 publications

Homoserine and asparagine are host signals that trigger in planta expression of a pathogenesis gene inNectria haematococca

Homoserine and asparagine are host signals that trigger in planta expression of a pathogenesis gene inNectria haematococca

Ustilago maydis – a valuable model system for the study of fungal dimorphism and virulence

Fuz1, a MYND domain protein, is required for cell morphogenesis inUstilago maydis

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