Completion of cell division is associated with maximum telomerase activity in naturally synchronized cultures of the green alga<i>Desmodesmus quadricauda</i>

Ševčíková, Tereza; Bišová, Kateřina; Fojtová, Miloslava; Lukešová, Alena; Hrčková, Kristýna; Sýkorová, Eva

doi:10.1016/j.febslet.2013.01.058

Cited by 8 publications

(2 citation statements)

References 34 publications

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“…Differences in the relative abundance of somatic versus stem cells might also determine variation in telomere length (Ojimi and Hidaka, 2010), but we cannot either discard recombination between homologous telomeres as a means to elongate telomeres (Liu et al, 2007). Even in plants, telomere restoration is only present in meristomatic and reproductive tissues, with exceptions in long-lived species (Flanary and Kletetschka, 2005;Watson and Riha, 2011 among other references), whereas in some algae it has been described telomerase activity during all life cycles, but large differences in telomerase activity have been found across algae groups (Fulnecková et al, 2013;Ševčíková et al, 2013). Further comparison of telomerase and stem cells in relation to regeneration in the analyzed populations of C. tenuispina would thus be of future interest.…”

Section: Discussionmentioning

confidence: 97%

Long telomeres are associated with clonality in wild populations of the fissiparous starfish Coscinasterias tenuispina

et al. 2015

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Telomeres usually shorten during an organism's lifespan and have thus been used as an aging and health marker. When telomeres become sufficiently short, senescence is induced. The most common method of restoring telomere length is via telomerase reverse transcriptase activity, highly expressed during embryogenesis. However, although asexual reproduction from adult tissues has an important role in the life cycles of certain species, its effect on the aging and fitness of wild populations, as well as its implications for the long-term survival of populations with limited genetic variation, is largely unknown. Here we compare relative telomere length of 58 individuals from four populations of the asexually reproducing starfish Coscinasterias tenuispina. Additionally, 12 individuals were used to compare telomere lengths in regenerating and non-regenerating arms, in two different tissues (tube feet and pyloric cecum). The level of clonality was assessed by genotyping the populations based on 12 specific microsatellite loci and relative telomere length was measured via quantitative PCR. The results revealed significantly longer telomeres in Mediterranean populations than Atlantic ones as demonstrated by the Kruskal-Wallis test (K = 24.17, significant value: P-valueo0.001), with the former also characterized by higher levels of clonality derived from asexual reproduction. Telomeres were furthermore significantly longer in regenerating arms than in non-regenerating arms within individuals (pyloric cecum tissue: Mann-Whitney test, V = 299, P-valueo10 − 6 ; and tube feet tissue Student's t = 2.28, P-value = 0.029). Our study suggests that one of the mechanisms responsible for the long-term somatic maintenance and persistence of clonal populations is telomere elongation.

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Section: Discussionmentioning

confidence: 97%

Long telomeres are associated with clonality in wild populations of the fissiparous starfish Coscinasterias tenuispina

et al. 2015

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“…In most cases, telomerase was enriched in a purified fraction, with the exception of protein extracts from chlorophytes and a glaucophyte alga, which showed telomerase activity also in the nonprecipitated fraction (supplementary table S3, Supplementary Material online). However, PEG purification led to removal of compounds inhibiting TRAP assay from these extracts (Ševčíková et al 2013). …”

Section: Discussionmentioning

confidence: 99%

A Broad Phylogenetic Survey Unveils the Diversity and Evolution of Telomeres in Eukaryotes

Fulnečková

Ševčíková

Fajkus

et al. 2013

Genome Biology and Evolution

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100

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Telomeres, ubiquitous and essential structures of eukaryotic chromosomes, are known to come in a variety of forms, but knowledge about their actual diversity and evolution across the whole phylogenetic breadth of the eukaryotic life remains fragmentary. To fill this gap, we employed a complex experimental approach to probe telomeric minisatellites in various phylogenetically diverse groups of algae. Our most remarkable results include the following findings: 1) algae of the streptophyte class Klebsormidiophyceae possess the Chlamydomonas-type telomeric repeat (TTTTAGGG) or, in at least one species, a novel TTTTAGG repeat, indicating an evolutionary transition from the Arabidopsis-type repeat (TTTAGGG) ancestral for Chloroplastida; 2) the Arabidopsis-type repeat is also present in telomeres of Xanthophyceae, in contrast to the presence of the human-type repeat (TTAGGG) in other ochrophytes studied, and of the photosynthetic alveolate Chromera velia, consistent with its phylogenetic position close to apicomplexans and dinoflagellates; 3) glaucophytes and haptophytes exhibit the human-type repeat in their telomeres; and 4) ulvophytes and rhodophytes have unusual telomere structures recalcitrant to standard analysis. To obtain additional details on the distribution of different telomere types in eukaryotes, we performed in silico analyses of genomic data from major eukaryotic lineages, utilizing also genome assemblies from our on-going genome projects for representatives of three hitherto unsampled lineages (jakobids, malawimonads, and goniomonads). These analyses confirm the human-type repeat as the most common and possibly ancestral in eukaryotes, but alternative motifs replaced it along the phylogeny of diverse eukaryotic lineages, some of them several times independently.

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Single-Run Catalysis and Kinetic Control of Human Telomerase Holoenzyme

Jiang

2021

Advances in Experimental Medicine and Biology

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Completion of cell division is associated with maximum telomerase activity in naturally synchronized cultures of the green algaDesmodesmus quadricauda

Cited by 8 publications

References 34 publications

Long telomeres are associated with clonality in wild populations of the fissiparous starfish Coscinasterias tenuispina

Long telomeres are associated with clonality in wild populations of the fissiparous starfish Coscinasterias tenuispina

A Broad Phylogenetic Survey Unveils the Diversity and Evolution of Telomeres in Eukaryotes

Single-Run Catalysis and Kinetic Control of Human Telomerase Holoenzyme

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