We study Gorenstein flat objects in the category Rep(Q, R) of representations of a left rooted quiver Q with values in Mod(R), the category of all left R-modules, where R is an arbitrary associative ring. We show that a representation X in Rep(Q, R) is Gorenstein flat if and only if for each vertex i the canonical homomorphism ϕ X i : ⊕ a:j→i X(j) → X(i) is injective, and the left R-modules X(i) and Coker ϕ X i are Gorenstein flat. As an application of this result, we show that there is a hereditary abelian model structure on Rep(Q, R) whose cofibrant objects are precisely the Gorenstein flat representations, fibrant objects are precisely the cotorsion representations, and trivial objects are precisely the representations with values in the right orthogonal category of all projectively coresolved Gorenstein flat left R-modules.