Complete Sequence and Gene Organization of the Nosema spodopterae rRNA Gene

Abstract

By sequencing the entire ribosomal RNA (rRNA) gene of Nosema spodopterae, we show here that its gene organization follows a pattern similar to the Nosema type species, Nosema bombycis, i.e. 5'-large subunit rRNA (2,497 bp)-internal transcribed spacer (185 bp)-small subunit rRNA (1,232 bp)-intergenic spacer (277 bp)-5S rRNA (114 bp)-3'. Gene sequences and the secondary structures of large subunit rRNA, small subunit rRNA, and 5S rRNA are compared with the known corresponding sequences and structures of closely related microsporidia. The results suggest that the Nosema genus may be heterogeneous and that the rRNA gene organization may be a useful characteristic for determining which species are closely related to the type species.

Ultrastructure, chromosomal karyotype, and molecular phylogeny of a new isolate of microsporidian Vairimorpha sp. BM (Microsporidia, Nosematidae) from Bombyx mori in China

“…Therefore, other markers were required for analyzing phylogenetic affinities. Tsai et al (2005) suggested that the microsporidian ITS and LSU rRNA sequences have good potential as informative molecular markers. According to the results of this study, the identity of the rRNA gene sequences of Vairimorpha sp.…”

Structure of ribosomal RNA gene and phytogeny ofNosema isolates in Korea

“…The reverse organization of rRNA of Nosema was suggested as distinct characteristics of the 'true' Nosema group (Tsai et al, 2005;Kyei-Poku et aI., 2008). Our phylogenetic analysis seems to support this idea, since all known Nosema species with rRNA reverse arrangement fall in the group.…”

Phylogenetic characterization of a microsporidium (Nosema sp.) isolated from the mulberry pest, Hemerophila atrilineata

“…cPP, Nosema sp. co and Nosema disstriae (Huang et al 2004, tsai et al 2005, Wang et al 2006, Ku et al 2007, Kyei-Poku et al 2008.…”

“…Tsai et al (2005) suggested that the reversed arrangement of rrNA genes such as lsU-ssU-5s arrangement may be a specific characteristic suitable for identification of Nosema species (Huang et al 2004(Huang et al , tsai et al 2005(Huang et al , ironside 2007. However, there are a few exceptions: refardt and Mouton (2007) and Xu et al (2011) reported a similar reverse rrNA arrangement of rrNA subunits in Glugoides intestinalis and Endoreticulatus sp., respectively.…”