Complete purification of the acetylcholine receptor protein from mammalian muscle

Dolly, J. Oliver; Barnard, Eric A.

doi:10.1016/0014-5793(75)80314-0

Cited by 27 publications

(6 citation statements)

References 16 publications

(8 reference statements)

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“…All these peaks were suppressed by inclusion of atropine when reacting the membranes with [3H]-PrBCM. This heterogeneity of specific labelling was highly suggestive of proteolysis of the receptor due to liberated proteases such as is well known to be a problem in attempting to isolate the nicotinic receptor from skeletal muscle (Dolly & Barnard, 1975). Addition of PMSF (I mM) during labelling confirmed this by increasing the labelling in the higher mol.…”

Section: Smooth Musclementioning

confidence: 67%

A Study of the Muscarinic Receptor by Gel Electrophoresis

Birdsall

Burgen

Hulme

1979

British J Pharmacology

112

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Muscarinic receptors from the brain of rat, guinea‐pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]‐PrBCM). After solubilisation and sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis a single radiolabeled peak was seen which was completely suppressed when labelling was carried out in the presence of 10−6 m atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of ∼80,000. In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.

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Section: Smooth Musclementioning

confidence: 67%

A Study of the Muscarinic Receptor by Gel Electrophoresis

Birdsall

Burgen

Hulme

1979

British J Pharmacology

112

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“…Annual Reviews www.annualreviews.org/aronline So far the main sources for the extraction of the receptor material have been homogenates of the electric organ of E. electricus and Torpedo species. Although other receptor-rich preparations have been investigated (74)(75)(76)(77)(78), the following discussion is limited to the AcCh-receptor system of Electrophorus and Torpedo, which have been studied more intensively. This receptor appears to be an integral membrane protein (79).…”

Section: The Acetylcholine Receptor Systemmentioning

confidence: 99%

Physical Chemistry of Excitable Biomembranes

Neumann¹,

Bernhardt²

1977

Annu. Rev. Biochem.

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“…In this paper, therefore, we report the characterization and purification of the ACh.R from denervated rat diaphragm and leg muscles of the cat, in the latter case to homogeneity, using gel filtration, biospecific chromatography and ion-exchange chromatography. A preliminary report of some of these findings has been made (Dolly and Barnard, 1975).…”

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confidence: 98%