Complete protection of mice against a lethal dose challenge of western equine encephalitis virus after immunization with an adenovirus-vectored vaccine

Wu, Josh; Barabé, Nicole D.; Chau, Damon; Wong, Christina; Rayner, George R.; Hu, Weigang; Nagata, Les P.

doi:10.1016/j.vaccine.2007.03.042

Cited by 29 publications

(18 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Israel, the fat sand rat (P. obesus), Sundevall's gerbil (Meriones crassus) and probably the short-tailed bandicoot rat (Nesokia indica) function as reservoirs for L. major (Schlein et al 1984). The same is happening in the Nile grass rat (Arvicanthis niloticus luctuosus) in Sudan (Hoogstraal and Heyneman 1969) and in Libyan jirds (Meriones libycus) and great gerbils (Rhombomys opimus) in Iran (Yaghoobi-Ershadi et al 1996) In Egypt, the great Egyptian gerbil (Gerbillus pyramidum) are carriers of L. major (Fryauff et al 1993). In the old world, black rats (R. rattus) are mentioned as incidental carriers of L. donovani (Ashford 1996).…”

Section: Leishmaniasismentioning

confidence: 98%

See 1 more Smart Citation

Rodent-borne diseases and their risks for public health

Meerburg¹,

Singleton²,

Kijlstra³

2009

Critical Reviews in Microbiology

775

722

View full text Add to dashboard Cite

Rodents are the most abundant and diversified order of living mammals in the world. Already since the Middle Ages we know that they can contribute to human disease, as black rats were associated with distribution of plague. However, also in modern times rodents form a threat for public health. In this review article a large number of pathogens that are directly or indirectly transmitted by rodents are described. Moreover, a simplified rodent disease model is discussed.

show abstract

Section: Leishmaniasismentioning

confidence: 98%

“…There is no commercial vaccine against WEEV and there are no licensed therapeutic drugs in the United States for this infection ). Consequently, scientists are working hard to develop a vaccine Wu et al 2007).…”

Section: Western Equine Encephalitismentioning

confidence: 99%

Rodent-borne diseases and their risks for public health

Meerburg¹,

Singleton²,

Kijlstra³

2009

Critical Reviews in Microbiology

775

722

View full text Add to dashboard Cite

show abstract

“…Subunit vaccines consisting of recombinant forms of WEEV E2 or E1 have been reported to induce significant protection in animal models (6)(7)(8). Although E2 is the major neutralizing antigen, E1 is highly conserved among alphaviruses (9,10).…”

mentioning

confidence: 99%

Liposome-Antigen-Nucleic Acid Complexes Protect Mice from Lethal Challenge with Western and Eastern Equine Encephalitis Viruses

Phillips

Schountz

Toth

et al. 2014

J Virol

View full text Add to dashboard Cite

Alphaviruses are mosquito-borne viruses that cause significant disease in animals and humans. Western equine encephalitis virus (WEEV) and eastern equine encephalitis virus (EEEV), two New World alphaviruses, can cause fatal encephalitis, and EEEV is a select agent of concern in biodefense. However, we have no antiviral therapies against alphaviral disease, and current vaccine strategies target only a single alphavirus species. In an effort to develop new tools for a broader response to outbreaks, we designed and tested a novel alphavirus vaccine comprised of cationic lipid nucleic acid complexes (CLNCs) and the ectodomain of WEEV E1 protein (E1ecto). Interestingly, we found that the CLNC component, alone, had therapeutic efficacy, as it increased survival of CD-1 mice following lethal WEEV infection. Immunization with the CLNC-WEEV E1ecto mixture (lipid-antigennucleic acid complexes [LANACs]) using a prime-boost regimen provided 100% protection in mice challenged with WEEV subcutaneously, intranasally, or via mosquito. Mice immunized with LANACs mounted a strong humoral immune response but did not produce neutralizing antibodies. Passive transfer of serum from LANAC E1ecto-immunized mice to nonimmune CD-1 mice conferred protection against WEEV challenge, indicating that antibody is sufficient for protection. In addition, the LANAC E1ecto immunization protocol significantly increased survival of mice following intranasal or subcutaneous challenge with EEEV. In summary, our LANAC formulation has therapeutic potential and is an effective vaccine strategy that offers protection against two distinct species of alphavirus irrespective of the route of infection. We discuss plausible mechanisms as well the potential utility of our LANAC formulation as a pan-alphavirus vaccine.

show abstract

“…The susceptibility of multiple mouse strains to WEEV and the responsiveness of mice to various routes of inoculation has given researchers great flexibility when choosing how to model WEEV disease in mice. The relative ease of replicating aspects of human WEEV disease in mice has allowed testing of a variety of vaccine candidates, including adenovirus vectors, DNA-based vaccines and live chimeric recombinant and subunit-based vaccines produced in E. coli (Atasheva et al, 2009;Barabé et al, 2007;Das et al, 2004Das et al, , 2007Gauci et al, 2010;Wu et al, 2007). In particular, 5-8-week-old C57BL/ 6 mice have been used extensively to test the efficacy of WEEV antivirals and vaccines.…”

Section: Western Equine Encephalitis Virusmentioning

confidence: 99%

Mouse models of alphavirus-induced inflammatory disease

Taylor

Herrero

Rudd

et al. 2015

Journal of General Virology

View full text Add to dashboard Cite

Part of the Togaviridae family, alphaviruses are arthropod-borne viruses that are widely distributed throughout the globe. Alphaviruses are able to infect a variety of vertebrate hosts, but in humans, infection can result in extensive morbidity and mortality. Symptomatic infection can manifest as fever, an erythematous rash and/or significant inflammatory pathologies such as arthritis and encephalitis. Recent overwhelming outbreaks of alphaviral disease have highlighted the void in our understanding of alphavirus pathogenesis and the re-emergence of alphaviruses has given new impetus to anti-alphaviral drug design. In this review, the development of viable mouse models of Old Word and New World alphaviruses is examined. How mouse models that best replicate human disease have been used to elucidate the immunopathology of alphavirus pathogenesis and trial novel therapeutic discoveries is also discussed.

show abstract

Complete protection of mice against a lethal dose challenge of western equine encephalitis virus after immunization with an adenovirus-vectored vaccine

Cited by 29 publications

References 34 publications

Rodent-borne diseases and their risks for public health

Rodent-borne diseases and their risks for public health

Liposome-Antigen-Nucleic Acid Complexes Protect Mice from Lethal Challenge with Western and Eastern Equine Encephalitis Viruses

Mouse models of alphavirus-induced inflammatory disease

Contact Info

Product

Resources

About