Complete parameterization of temporally and spectrally resolved laser induced fluorescence data with applications in bio-photonics

Brydegaard, Mikkel; Thompson, Alexander; Andersson‐Engels, Stefan; Bendsöe, Niels; Svanberg, Katarina; Svanberg, Sune

doi:10.1016/j.chemolab.2015.01.014

Cited by 2 publications

(1 citation statement)

References 88 publications

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“…The time-resolved spectroscopic techniques usually require a more complex instrumentation but can investigate much more intrinsic properties of the sample, being less affected by absorption and scattering on both the excitation and emission wavelengths. 33 A brief overview on the fluorescence dynamics and on the time-resolved spectroscopy technique will be presented on the following sections.…”

Section: Basic Principles Of Fluorescencementioning

confidence: 99%

Assembly, characterization, and validation of a fluorescence lifetime rigid endoscope for clinical imaging of skin lesions

Rosa¹

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Fluorescence based microscopy techniques have been extensively used in biological sciences. The most common approach is the steady-state fluorescence microscopy. Although the said approach is powerful, it often lacks sensitivity to detect several biochemical processes that may indicate relevant conditions of biological tissues. The fluorescence dynamics analysis not only brings intrinsic information about the tissue, but is also less sensitive to the medium scattering and absorption, and sometimes capable of distinguishing between fluorescent structures with indistinguishable spectra. The intrinsic fluorescence lifetime of biological tissues is usually affected by some clinical conditions, especially when those conditions cause or are correlated with metabolic modifications. Time-resolved spectroscopy techniques can be used to detect those modifications and may be used as a tool to improve the detection and diagnosis rate of such conditions. Fluorescence Lifetime Imaging Microscopy (FLIM) combines the temporal resolution and the microscopy concept, so fluorescence lifetime images can be generated. This technique has a great potential for clinical applications since it may be able to detected lesions and delineate its borders. However, FLIM usually demands a more sophisticated instrumentation than most techniques based on the steady-state approach, what creates a difficulty for moving such a system to a clinical setting. We report the assembly, characterization, validation, and clinical application of a multispectral FLIM system featuring a handheld probe composed of a laser scanning rigid endoscope. The assembled system uses a 355 short pulsed laser as excitation and has three spectral channels, targeting the emission of collagen, NADH, and FAD, which are important endogenous fluorophores. The system acquires images of 8.65 × 8.65 2 areas in ∼ 2.4. MATLAB codes were written to process the images using a biexponential model and a modified phasor approach. In vivo validation measurements of tumors induced in mice were performed. The system was also validated with in vivo imaging of skin of healthy volunteers. The assembled FLIM system was moved to Hospital Amaral Carvalho, where we performed a pilot clinical study, in which different types of skin lesions were imaged in vivo in a clinical setting. A significant contrast was achieved on seborrheic keratosis, Bowen's disease, and sclerodermiform basal cell carcinoma tumors. These results indicate the potential of this technique for clinical imaging of skin lesions.

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Section: Basic Principles Of Fluorescencementioning

confidence: 99%

Assembly, characterization, and validation of a fluorescence lifetime rigid endoscope for clinical imaging of skin lesions

Rosa¹

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Optical detection of middle ear infection using spectroscopic techniques: phantom experiments

Zhang

Huang

et al. 2015

J. Biomed. Opt

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A noninvasive optical technique, which is based on a combination of reflectance spectroscopy and gas in scattering media absorption spectroscopy, is demonstrated. It has the potential to improve diagnostics of middle ear infections. An ear phantom prepared with a tissue cavity, which was covered with scattering material, was used for spectroscopic measurements. Diffuse reflectance spectra of the phantom eardrum were measured with a reflectance probe. The presence of oxygen and water vapor as well as gas exchange in the phantom cavity were studied with a specially designed fiber-optic probe for backscattering detection geometry. The results suggest that this method can be developed for improved clinical detection of middle ear infection.

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Complete parameterization of temporally and spectrally resolved laser induced fluorescence data with applications in bio-photonics

Cited by 2 publications

References 88 publications

Assembly, characterization, and validation of a fluorescence lifetime rigid endoscope for clinical imaging of skin lesions

Assembly, characterization, and validation of a fluorescence lifetime rigid endoscope for clinical imaging of skin lesions

Optical detection of middle ear infection using spectroscopic techniques: phantom experiments

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