Complete oxidation of catechol by the strictly anaerobic sulfate-reducing Desulfobacterium catecholicum sp. nov.

Szewzyk, Regine; Pfennig, Norbert

doi:10.1007/bf00415278

Cited by 126 publications

(56 citation statements)

References 34 publications

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“…4). This FA was reported to occur in Desulfobacter and Desulfobacula Kuever et al, 2001), both strictly anaerobic organisms (Szewzyk and Pfennig, 1987;Widdel, 1987;Kuever et al, 2001). Indeed, Desulfobacula toluolica was genetically identified by Labrenz et al (2007) in suboxic and anoxic waters of the central Baltic Sea.…”

Section: Microbial Processes In the Anoxic Zonementioning

confidence: 96%

Biomarkers in the stratified water column of the Landsort Deep (Baltic Sea)

et al. 2014

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Abstract. The water column of the Landsort Deep, central Baltic Sea, is stratified into an oxic, suboxic, and anoxic zone. This stratification controls the distributions of individual microbial communities and biogeochemical processes. In summer 2011, particulate organic matter was filtered from these zones using an in situ pump. Lipid biomarkers were extracted from the filters to establish water-column profiles of individual hydrocarbons, alcohols, phospholipid fatty acids, and bacteriohopanepolyols (BHPs). As a reference, a cyanobacterial bloom sampled in summer 2012 in the central Baltic Sea Gotland Deep was analyzed for BHPs. The biomarker data from the surface layer of the oxic zone showed major inputs from cyanobacteria, dinoflagellates, and ciliates, while the underlying cold winter water layer was characterized by a low diversity and abundance of organisms, with copepods as a major group. The suboxic zone supported bacterivorous ciliates, type I aerobic methanotrophic bacteria, sulfate-reducing bacteria, and, most likely, methanogenic archaea. In the anoxic zone, sulfate reducers and archaea were the dominating microorganisms as indicated by the presence of distinctive branched fatty acids: archaeol and pentamethylicosane (PMI) derivatives, respectively. Our study of in situ biomarkers in the Landsort Deep thus provided an integrated insight into the distribution of relevant compounds and describes useful tracers to reconstruct stratified water columns in the geological record.

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Section: Microbial Processes In the Anoxic Zonementioning

confidence: 96%

Biomarkers in the stratified water column of the Landsort Deep (Baltic Sea)

et al. 2014

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“…Molecular characterization of a sulfate-reducing enrichment culture degrading benzene identified clones related to Cytophaga, Thiomicrospora, low G þ C Grampositives and Deltaproteobacteria (Phelps et al, 1998) of which four major clones fell within the deltaproteobacterial Desulfobacteraceae, members of which are known to utilize aromatic compounds (Bak and Widdel, 1986;Szewzyk and Pfennig, 1987;Rabus et al, 1993;Beller et al, 1996).…”

Section: Desulfitobacterium Chlororespirans (T) U68528 Desulfosporosmentioning

confidence: 99%

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

2007

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Here, we present a detailed functional and phylogenetic characterization of an iron-reducing enrichment culture maintained in our lab with benzene as sole carbon and energy source. We used DNA-stable isotope probing to identify microbes within the enrichment most active in the assimilation of 13 C-label. When 12 C 6 -and 13 C 6 -benzene were added as comparative substrates, marked differences in the quantitative buoyant density distribution became apparent especially for uncultured microbes within the Gram-positive Peptococcaceae, closely related to environmental clones retrieved from contaminated aquifers world wide and only distantly related to cultured representatives of the genus Thermincola. Prominent among the other constituents of the enrichment were uncultured Deltaproteobacteria, as well as members of the Actinobacteria. Although their presence within the enrichment seems to be stable they did not assimilate 13 C-label as significantly as the Clostridia within the time course of our experiment. We hypothesize that benzene degradation in our enrichment involves an unusual syntrophy, where members of the Clostridia primarily oxidize benzene. Electrons from the contaminant are both directly transferred to ferric iron by the primary oxidizers, but also partially shared with the Desulfobulbaceae as syntrophic partners. Alternatively, electrons may also be quantitatively transferred to the partners, which then reduce the ferric iron. Thus our results provide evidence for the importance of a novel clade of Gram-positive iron-reducers in anaerobic benzene degradation, and a role of syntrophic interactions in this process. These findings shed a totally new light on the factors controlling benzene degradation in anaerobic contaminated environments.

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“…There are many examples in the literature of sulfate-reducing bacteria that are also able to reduce nitrate (14,16,17,27,31,38), but this capacity is absent from the two strains of Desulfovibrio vulgaris for which complete genome sequence data are available (12). There is little agreement concerning how nitrate reduction is regulated, even in strains of D. desulfuricans.…”

mentioning

confidence: 99%

Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

2009

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Desulfovibrio desulfuricans strain 27774 is one of a relative small group of sulfate-reducing bacteria that can also grow with nitrate as an alternative electron acceptor, but how nitrate reduction is regulated in any sulfate-reducing bacterium is controversial. Strain 27774 grew more rapidly and to higher yields of biomass with nitrate than with sulfate or nitrite as the only electron acceptor. In the presence of both sulfate and nitrate, sulfate was used preferentially, even when cultures were continuously gassed with nitrogen and carbon dioxide to prevent sulfide inhibition of nitrate reduction. The napC transcription start site was identified 112 bases upstream of the first base of the translation start codon. Transcripts initiated at the napC promoter that were extended across the napM-napA boundary were detected by reverse transcription-PCR, confirming that the six nap genes can be cotranscribed as a single operon. Real-time PCR experiments confirmed that nap operon expression is regulated at the level of mRNA transcription by at least two mechanisms: nitrate induction and sulfate repression. We speculate that three almost perfect inverted-repeat sequences located upstream of the transcription start site might be binding sites for one or more proteins of the CRP/FNR family of transcription factors that mediate nitrate induction and sulfate repression of nitrate reduction by D. desulfuricans.

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Complete oxidation of catechol by the strictly anaerobic sulfate-reducing Desulfobacterium catecholicum sp. nov.

Cited by 126 publications

References 34 publications

Biomarkers in the stratified water column of the Landsort Deep (Baltic Sea)

Biomarkers in the stratified water column of the Landsort Deep (Baltic Sea)

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

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