Complete nucleotide sequences of two begomoviruses infecting Madagascar periwinkle (Catharanthus roseus) from Pakistan

Abstract

Though Catharanthus roseus (Madagascar periwinkle) is an ornamental plant, it is famous for its medicinal value. Its alkaloids are known for anti-cancerous properties, and this plant is studied mainly for its alkaloids. Here, this plant has been studied for its viral diseases. Complete DNA sequences of two begomoviruses infecting C. roseus originating from Pakistan were determined. The sequence of one begomovirus (clone KN4) shows the highest level of nucleotide sequence identity (86.5 %) to an unpublished virus, chili leaf curl India virus (ChiLCIV), and then (84.4 % identity) to papaya leaf curl virus (PaLCV), and thus represents a new species, for which the name "Catharanthus yellow mosaic virus" (CYMV) is proposed. The sequence of another begomovirus (clone KN6) shows the highest level of sequence identity (95.9 % to 99 %) to a newly reported virus from India, papaya leaf crumple virus (PaLCrV). Sequence analysis shows that KN4 and KN6 are recombinants of Pedilanthus leaf curl virus (PedLCV) and croton yellow vein mosaic virus (CrYVMV).

First report on the association of a begomovirus with chrysanthemum indicum exhibiting yellowing of leaf vein disease characterized by molecular studies

“…There are reports of begomovirus infection in ornamental plants worldwide (He et al 2009;Ilyas et al 2013). With regard to the Indian context, numerous reports on begomovirus infection in weeds and crops are available (Chatterjee et al 2007;Das et al 2008;Ghosh et al 2007;Jyothsna et al 2011;Khan et al 2002) but there is much less information regarding begomovirus infections in ornamental plants (Marwal et al 2013b).…”

Emerging threats of begomoviruses to the cultivation of medicinal and aromatic crops and their management strategies

“…Genomic DNA was isolated and subjected to rolling circle amplification (RCA) and restricted with different enzymes and with enzyme XhoI, a band of 2.8 Kb was obtained. Two clones are obtained and sequenced (KN4 and KN6) [67]. Two clones are obtained and sequenced (KN4 and KN6) [67].…”

“…Two clones are obtained and sequenced (KN4 and KN6) [67]. Two clones are obtained and sequenced (KN4 and KN6) [67]. BLAST analysis showed that the sequence of clone KN4 has less than 89% identity to an unpublished virus, Chilli leaf curl India virus (ChiLCIV-[IN:08] FM877858) and the sequence of clone KN6 has 95 and 99% identity to a new reported virus Papaya leaf crumple virus (PaLCrV) HM140369.…”

“…BLAST analysis showed that the sequence of clone KN4 has less than 89% identity to an unpublished virus, Chilli leaf curl India virus (ChiLCIV-[IN:08] FM877858) and the sequence of clone KN6 has 95 and 99% identity to a new reported virus Papaya leaf crumple virus (PaLCrV) HM140369. KN4 represents an isolate of a newly identified species in the genus Begomovirus, and thus named as ''Catharanthus yellow mosaic virus'' (CYMV) [67]. No DNA-B and betasatellite presence was found.…”

Begomovirus associated with alternative host weeds: a critical appraisal

“…Several researchers' reviews on begomovirus covered different aspects of their biology, molecular biology, epidemiology and serological properties (Hanley-Bowdoin et al 1999;Harrison & Robinson, 1999;Faria et al 2000;Morales & Anderson, 2001;Ilyas et al 2013). Different known symptoms that associated with begomovirus infection have been observed in plants grown in tropical and subtropical regions of the world since the mid1800s (Wege et al 2000;Goodman 1977a;Harrison et al 1977).…”