Complete Nucleotide Sequences of
            <i>bla</i>
            <sub>CTX-M</sub>
            -Harboring IncF Plasmids from Community-Associated Escherichia coli Strains in the United States

Li, Jun Jie; Spychala, Caressa N.; Hu, Fupin; Sheng, Ji fang; Doi, Yohei

doi:10.1128/aac.04772-14

Cited by 40 publications

(34 citation statements)

References 28 publications

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“…These ␤-lactamases have become the predominant family of ESBLs in many regions of the world (24). In clinical strains, bla CTX-M enzymes are encoded most commonly within plasmids that carry additional genes for resistance to other antimicrobials, including aminoglycosides, chloramphenicol, trimethoprim-sulfamethoxazole, fluoroquinolones, tetracycline, and 16S RNA methylase genes, as well as housing other ␤-lactamase genes, with increasing reports in China and the developing world (25)(26)(27)(28)(29), as well as the United States (30,31).…”

mentioning

confidence: 99%

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

McCollister

Kotter

Frank

et al. 2016

Antimicrob Agents Chemother

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bWe report a case of ceftriaxone treatment failure for bacteremia caused by Salmonella enterica subsp. enterica serovar Typhimurium, due to the in vivo acquisition of a bla CTX-M-27 -encoding IncFII group transmissible plasmid. The original ␤-lactamasesusceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to Escherichia coli at 37°C. We used whole-genome sequencing of ST882S and ST931R, the E. coli transconjugant, and isolated plasmid DNA to unequivocally show that ST882S and ST931R had identical chromosomes, both having 206 identical single-nucleotide polymorphisms (SNPs) versus S. Typhimurium 14028s. We assembled a complete circular genome for ST931R, to which ST882S reads mapped with no SNPs. ST882S and ST931R were isogenic except for the presence of three additional plasmids in ST931R. ST931R and the E. coli transconjugant were ceftriaxone resistant due to the presence of a 60.5-kb IS26-flanked, bla CTX-M-27 -encoding IncFII plasmid. Compared to 14082s, ST931R has almost identical Gifsy-1, Gifsy-2, and ST64B prophages, lacks Gifsy-3, and instead carries a unique Fels-2 prophage related to that found in LT2. ST882S and ST931R both had a 94-kb virulence plasmid showing >99% identity with pSLT14028s and a cryptic 3,904-bp replicon; ST931R also has cryptic 93-kb IncI1 and 62-kb IncI2 group plasmids. To the best of our knowledge, in vivo acquisition of extended-spectrum ␤-lactamase resistance by S. Typhimurium and bla CTX-M-27 genes in U.S. isolates of Salmonella have not previously been reported. G reater than 1.4 million cases of nontyphoidal salmonellosis are estimated to occur each year in the United States, with approximately 95% of cases resulting from foodborne transmission (1). Although most infections result in mild to moderate gastroenteritis that usually resolves with or without treatment, concurrent bacteremia is observed in approximately 6% of patients and can be associated with serious disease, especially in infants aged Ͻ1 year, the elderly, and those with immunocompromised states such as HIV infection (2-5). Although routine use of antimicrobial therapy is not generally recommended for the treatment of most Salmonella infections, such therapy may be lifesaving in persons with invasive disease.Treatment of invasive salmonellosis has been compromised in recent years due to the emergence of Salmonella isolates with single or multidrug resistance to a number of first-line agents (6, 7). Antimicrobial resistance in strains of nontyphoidal Salmonella (NTS) has been linked to the use of antimicrobial agents in livestock (8-11). Consequently, agents such as fluoroquinolones and third generation cephalosporins, such as ceftriaxone, have become treatment modalities of choice for therapy against severe Salmonella infections. In the United States before 1996, all reported cases of infection with ceftriaxone-resistant NTS isolates were known o...

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mentioning

confidence: 99%

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

McCollister

Kotter

Frank

et al. 2016

Antimicrob Agents Chemother

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“…These plasmids have been largely reported in clinical isolates from humans around the world and more recently from cattle and pets in France, and from the fecal flora of healthy calves in the Netherlands (http:// pubmlst.org/plasmid/) Dahmen et al, 2013a;Hordijk et al, 2013). Their presence in different pathogen species and in different countries (Villa et al, 2010;Dahmen et al, 2013a;Li et al, 2015;Rafai et al, 2015) suggests that horizontal transfer of plasmids rather than clonal dissemination is probably an important mechanism of bla CTX-M-15 spread.…”

Section: Characterization Of Bla Ctx-m-15 -Carrying E Coli and Plasmidsmentioning

confidence: 99%

Dominant plasmids carrying extended‐spectrum β‐lactamases bla_CTX‐M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters

Dupouy

Doublet

Arpaillange

et al. 2016

Environ Microbiol Rep

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Wastewater treatment plants (WWTP) receiving effluents from food-producing animals and humans may contribute to the spread of extended-spectrum β-lactamases (ESBL)-carrying plasmids. This study was designed to investigate extended-spectrum cephalosporin resistant Escherichia coli strains, CTX-M distributions and the genetic lineage of bla -carrying plasmids from urban and slaughterhouse wastewaters. The level of extended-spectrum cephalosporin-resistant E. coli in slaughterhouse wastewater entering the WWTP was negligible compared with that of urban wastewater. The bla gene was predominant in slaughterhouse wastewater whereas diverse bla genes were encountered in urban wastewater and WWTP outlet. Characterization of the main CTX-M-producing E. coli isolates by antibiotic resistance phenotyping, genotyping and typing of plasmids carrying bla genes revealed that bla and bla genes were harboured by the predominant bla IncI1/ST3 and bla F31:A4:B1 plasmids, which were recovered from unrelated E. coli genotypes in both slaughterhouse and urban wastewaters. This study highlighted the spread of predominant bla and bla plasmid lineages in diverse E. coli genotypes from humans and food-producing animals, their mixing in WWTP and final release into the aquatic environment. This could have a serious negative impact on public health and requires further evaluation.

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“…Plasmid copy number, replication efficiency, post-segregational killing, and an effective decrease in partition units due to concatemerization or clustering are all variables that have been considered (Bergstrom et al, 2000; Cooper et al, 1987; De Gelder et al, 2007; Lau et al, 2013; Lili et al, 2007; San Millan et al, 2014; Werbowy et al, 2017). Given that naturally-occurring plasmids typically experience fluctuating selective pressures and would be susceptible to loss over long periods of time, these plasmids typically have mechanisms modulating one or more of these variables to enhance their stability (Li et al, 2015; Lobato-Marquez et al, 2016; Werbowy and Kaczorowski, 2016). These stabilizing mechanisms include plasmid addiction systems that kill plasmidless daughter cells (Kroll et al, 2010; Li et al, 2015; Mruk and Kobayashi, 2014; Werbowy and Kaczorowski, 2016), multimer resolution systems to suppress the formation of concatemers by homologous recombination (Summers et al, 1993; Werbowy et al, 2015), and the presence of multiple redundant mechanisms for replication initiation (Cesareni et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Genetic control of ColE1 plasmid stability that is independent of plasmid copy number regulation

et al. 2018

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ColE1-like plasmid vectors are widely used for expression of recombinant genes in E. coli. For these vectors, segregation of individual plasmids into daughter cells during cell division appears to be random, making them susceptible to loss over time when no mechanisms ensuring their maintenance are present. Here we use the plasmid pGFPuv in a recA relA strain as a sensitized model to study factors affecting plasmid stability in the context of recombinant gene expression. We find that in this model, plasmid stability can be restored by two types of genetic modifications to the plasmid origin of replication (ori) sequence: point mutations and a novel 269 nt duplication at the 5' end of the plasmid ori, which we named DAS (duplicated anti-sense) ori. Combinations of these modifications produce a range of copy numbers and of levels of recombinant expression. In direct contradiction with the classic random distribution model, we find no correlation between increased plasmid copy number and increased plasmid stability. Increased stability cannot be explained by reduced levels of recombinant gene expression either. Our observations would be more compatible with a hybrid clustered and free-distribution model, which has been recently proposed based on detection of individual plasmids in vivo using super-resolution fluorescence microscopy. This work suggests a role for the plasmid ori in the control of segregation of ColE1 plasmids that is distinct from replication initiation, opening the door for the genetic regulation of plasmid stability as a strategy aimed at enhancing large-scale recombinant gene expression or bioremediation.

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Complete Nucleotide Sequences of bla _CTX-M -Harboring IncF Plasmids from Community-Associated Escherichia coli Strains in the United States

Cited by 40 publications

References 28 publications

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Dominant plasmids carrying extended‐spectrum β‐lactamases bla_CTX‐M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters

Genetic control of ColE1 plasmid stability that is independent of plasmid copy number regulation

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Complete Nucleotide Sequences of bla CTX-M -Harboring IncF Plasmids from Community-Associated Escherichia coli Strains in the United States

Cited by 40 publications

References 28 publications

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Dominant plasmids carrying extended‐spectrum β‐lactamases blaCTX‐M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters

Genetic control of ColE1 plasmid stability that is independent of plasmid copy number regulation

Contact Info

Product

Resources

About

Complete Nucleotide Sequences of bla _CTX-M -Harboring IncF Plasmids from Community-Associated Escherichia coli Strains in the United States

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

Dominant plasmids carrying extended‐spectrum β‐lactamases bla_CTX‐M genes in genetically diverse Escherichia coli from slaughterhouse and urban wastewaters