Complete Nucleotide Sequences of bla _KPC-4 - and bla _KPC-5 -Harboring IncN and IncX Plasmids from Klebsiella pneumoniae Strains Isolated in New Jersey

Abstract

h Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla KPC , commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla KPC-2 and bla KPC-3 , identified in plasmids with diverse genetic backgrounds. In this study, we examined bla KPC-4 -and bla KPC-5 -bearing plasmids recovered from two K. pneumoniae strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors bla KPC-4 , bla TEM-1 , qnrB2, aac(3)-Ib, aph(3=)-I, qacF, qacE⌬1, sul1, and dfrA14, which confer resistance to ␤-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn4401 sequence revealed a large IS110-and Tn6901-carrying element (8.3 kb) inserted into the istA gene, encoding glyoxalase/bleomycin resistance, alcohol dehydrogenase, and S-formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors bla KPC-5 , dfrA5, qacE⌬1, and sul1. pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The bla KPC-5 gene is carried on a Tn4401 element and differs from the genetic environment of bla KPC-5 described in Pseudomonas aeruginosa strain P28 from Puerto Rico. This study underscores the genetic diversity of multidrug-resistant plasmids involved in the spread of bla KPC genes and highlights the mobility and plasticity of Tn4401. Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups.

Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco

“…Classes 1, 2, and 3 are the most commonly detected 19 . Class 1 integrons are the most widespread and have been frequently found in extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Enterobacteriaceae, including K. pneumoniae 6,13,16,20,21 . Class 2 integrons occur less frequently in ESBL-producing Escherichia coli and K. pneumoniae, and class 3 integrons are rarely found in ESBL-producing K. pneumoniae 21,22 .…”

“…This species is classifi ed into three phylogenetic groups, KpI, KpII, and KpIII, based on nucleotide variations in the constitutively expressed genes gyrA, parC, and rpoB 4,5 . Mobile genetic elements, such as integrons, contribute to the evolution and dissemination of multidrug resistance genes (bla CTX-M, bla IMP, and bla GES ) in K. pneumoniae through horizontal or vertical transfer [6][7][8][9] . The mobile class 1 integrons are predominantly found in clinical isolates and are associated with transposon Tn21.…”

Characterization of Tn 3000 , a Transposon Responsible for bla NDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

“…It has a complete pilX operon, encoding a conjugation apparatus, and also taxA, taxB, and taxC genes, implicated in plasmid transfer. The taxC gene sequence was compared to IncX plasmids recently reviewed (46), and the highest similarity was observed with IncX3 plasmids pEC14_35 (GenBank accession no. JN935899) (95.4%) (47) and pIncX-SHV (GenBank accession no.…”

Molecular Survey of the Dissemination of Two bla KPC -Harboring IncFIA Plasmids in New Jersey and New York Hospitals

“…Presently, bla KPC is identified on plasmids of different incompatibility (Inc) replicon groups, including IncFII, IncI2, IncL/M, IncN, IncA/C, IncR, IncX, and ColE1 (4,(6)(7)(8)(9). Among them, certain plasmids appear to be epidemic, contributing significantly to the spread of KPC.…”