Complete nucleotide sequence of the ribosomal RNA tandem repeat unit from<i>Giardia intestinalis</i>

Healey, Andrew; Mitchell, Richard R.; Upcroft, Jacqueline; Boreham, P. F. L.; Upcroft, Peter

doi:10.1093/nar/18.13.4006

Cited by 63 publications

(30 citation statements)

References 5 publications

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“…There are -60 copies of a 5.6 kb rRNA gene (rDNA) repeat unit in the G.lamblia genome (9,24,25). These rRNA genes are arranged in short tandem arrays located on at least six telomeres (referred to as rDNA-telomere domains) (19,22,26).…”

Section: Introductionmentioning

confidence: 99%

Structure of a frequently rearranged rRNA-encoding chromosome inGiardia lamblia

Hou¹,

Blancq

Yaping

et al. 1995

Nucl Acids Res

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Section: Introductionmentioning

confidence: 99%

Structure of a frequently rearranged rRNA-encoding chromosome inGiardia lamblia

Hou¹,

Blancq

Yaping

et al. 1995

Nucl Acids Res

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“…(1) Thompson et al (2000), (2) Sogin et al (1989), (3) van Keulen et al (1995), (4) Not yet published, Xiao, S., South China Agricultural University, 2005, (5) Healey et al (1990), (6) , (7) Monis et al (1999), (8) (2000), (20) Yee and Dennis (1992), (21) Ey et al (1997), (22) Leonhard et al (2006), (23) Monis et al (1996), (24) Ey, P., University of Adelaide, 2002, submitted as a set with those from Ey et al (1997), (25) , (26) Itagaki et al (2005), (27) Matsubayashi et al (2005), (28) Robertson et al (2006), (29) Mowatt et al (1994), (30) Trout et al (2003), (31) Baruch et al (1996), (32) Sulaiman et al (2003), (33) Sulaiman et al (2004), (34) Not yet published, Mowatt, M., National…”

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Comparative evaluation ofGiardia duodenalissequence data

2007

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A review of the Giardia duodenalis sequences currently available on the GenBank database was completed to compare the different genotyping loci (small subunit ribosomal DNA, glutamate dehydrogenase, triose-phosphate isomerase and beta giardin) for their ability to discern assemblage and subassemblage groups and infer phylogenetic relationships. In total, 405 Giardia duodenalis sequences were sorted and aligned to examine the substitutions within and between the assemblages -A and B (zoonotic), C and D (dogs), E (livestock), F (cats) and G (rodents). It was found that all of the genes could reproducibly group isolates into their assemblages and that the AI/AII subassemblage groups were robust and identifiable at all loci. However, the assemblage B subgroups were not reproducible at half of the loci (small subunit ribosomal DNA and beta giardin), not due to their conserved nature, but because there was insufficient sequence data of reference isolates available for comparison. It is anticipated that further investigation of these loci may reveal the core subgroups of this medically important and zoonotic assemblage and also those of others. The closer, more recent, phylogenetic relationships amongst the assemblages appear to be resolved ; however, more sequence data from the current loci, and possibly new loci, will be required to establish the remaining relationships.

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“…If, however, the chromosomes carrying complete rRNA gene units are cleaved with NotI, more than 4,000 bp of the rRNA gene units will remain associated with the TGU and other chromosomal DNA, while the telomere repeats will be found on chromosome segments of less than 30 kb. The rRNA gene units from accessory chromosomes carrying tandemly arrayed rRNA genes will mostly be reduced to individual rRNA gene units of 5,566 bp (5).…”

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Rearranged Subtelomeric rRNA Genes in Giardia duodenalis

2005

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Giardia duodenalis has linear chromosomes capped with typical eukaryotic repeats [(TAGGG) n ], subtelomeric rRNA genes, and telomere gene units. The absence of two closely associated NotI sites in the large-subunit rRNA gene was used as an indicator in hybridizations of one- and two-dimensional NotI-cleaved Giardia chromosome separations that some chromosomes carry only rearranged and, by deduction, nonfunctional rRNA genes.

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Complete nucleotide sequence of the ribosomal RNA tandem repeat unit fromGiardia intestinalis

Cited by 63 publications

References 5 publications

Structure of a frequently rearranged rRNA-encoding chromosome inGiardia lamblia

Structure of a frequently rearranged rRNA-encoding chromosome inGiardia lamblia

Comparative evaluation ofGiardia duodenalissequence data

Rearranged Subtelomeric rRNA Genes in Giardia duodenalis

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