Complete nucleotide sequence of the Japanese isolate S of beet necrotic yellow vein virus RNA and comparison with European isolates

Abstract

The complete nucleotide sequences of beet necrotic yellow vein virus RNA-1 to RNA-4 of the Japanese isolate S (BNYVV-S) were determined and compared with those of French isolate (BNYVV-F2). The nucleotide sequences of the two isolates were very similar, differing by only 1.7% (RNA-1), 4.1% (RNA-2), 2.9% (RNA-3) and 3.6% (RNA-4), respectively. The differences of the amino acid sequences of the two isolates depended upon the open reading frames (ORF) as follows: P237, 1.4%; P22 (coat protein), 2.1%; 54k ORF, 3.4%; P42, 0.5%; P13, 1.7%; P15, 3.0%; P14, 7.0% P25, 6.4%; P31, 3.5%. Comparison of the coat protein and triple gene block (P42, P13 and P15) regions of RNA-2 with other isolates revealed that BNYVV-S was much more similar to the Yugoslavian isolate (BNYVV-Yu2) than to BNYVV-F2. The nucleotide differences between BNYVV-S and BNYVV-Yu2 were less than 1%. Based upon the grouping of BNYVV variants reported by Kruse et al. [10], BNYVV-S is thus considered to belong to the A type along with BNYVV-Yu2, whereas BNYVV-F2 is classified in the B type. Our data suggest that the Japanese isolate S may have been derived from European countries other than France or Germany.

Construction of Plant Transformation Vectors Carrying Beet Necrotic Yellow Vein Virus Coat Protein Gene (I) - Transformation Vectors

“…CPL and CPS sequences had very high homology level with BNYVV coat protein gene sequences in data bases, which confirms the fact that differences in BNYVV coat protein gene between different isolates are very small (24), and confirms the fact that sequence of this gene can remain unchanged for long period of time (16). Although sequences of our fragments had high homology with other sequences of BNYVV coat protein gene, it differed from all of them in two nucleotides 273 and 519, which could be considered as specific feature of this isolate.…”

Identification of amino acids of the beet necrotic yellow vein virus p25 protein required for induction of the resistance response in leaves of Beta vulgaris plants

“…Sequences of RNA3 from natural BNYVV isolates were obtained as described previously (Miyanishi et al, 1999). Two specific primers, 3F (59-AGTTGTTGTGTTTTCTGATC-39, nt 4082427) and the reverse primer 3R (59-CCGTGAAATCACGTGTAGTT-39, complementary to nt 124921268) were used (Saito et al 1996). PCR products were ligated into a pGEM-T vector (Promega) and transformed into Escherichia coli strain XL1-Blue.…”

“…Total RNA was extracted from 150-300 mg leaf and root tissues. The blot was hybridized with digoxigenin-labelled DNA probes specific for the BNYVV RNA1 (nt 581526531), RNA2 (nt 1442711) and RNA3 sequences (nt 44421104) (Saito et al, 1996). Equal loading was verified by visualization of ethidium bromide-stained 28S rRNA.…”

Diversity and evolution of potato mop-top virus

“…However, the 8K protein appears to be less conserved between the two distinct groups. This has also been observed for other proteins with silencing suppressor activities, such as 16K of tobacco rattle virus and p14 of beet necrotic yellow vein virus [4,18]. Because the 8K protein acts as a virulence factor [13], the differences …”