Complete nucleotide sequence of pig (Sus scrofa) mitochondrial genome and dating evolutionary divergence within Artiodactyla

Lin, Chih‐Sheng; Sun, Yu; Liu, Chang Yeu; Yang, Ping; Chang, Ling; Cheng, Ivan Cheng; Mao, Simon J.T.; Huang, Mengyi

doi:10.1016/s0378-1119(99)00247-4

Cited by 72 publications

(40 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative real-time polymerase chain reaction (qRT-PCR) From the 10 analyzed genes, seven were chosen based on Lin et al (1999), and their sequences were obtained from the National Center for Biotechnology Information database (http://www.ncbi.nlm.nih.gov; Accession No. AF034253).…”

Section: Methodsmentioning

confidence: 99%

“…AF034253). The sequences based on Lin et al (1999) consisted of seven subunits from enzymatic complexes in the mitochondrial respiratory cascade: ND1, NADH dehydrogenase subunit 1, and ND2, NADH dehydrogenase subunit 2, both from the NADH ubiquinone oxidoreductase (complex I); CYTB, Cytochrome b, from the ubiquinone cytochrome c reductase (complex III); COX1, Cytochrome oxidase subunit 1; COX2, Cytochrome subunit 2; and COX3, Cytochrome subunit 3; from Cytochrome c oxidase (complex IV); and ATP6, ATPase subunit 6, from the F 1 F 0 ATPase complex (complex V).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Weller

Alebrante

Campos

et al. 2013

Animal

View full text Add to dashboard Cite

The purpose of this study was to evaluate the effect of temperature and different levels of available phosphorus (aP) on the expression of nine genes encoding electron transport chain proteins in the Longissimus dorsi (LD) muscle of pigs. Two trials were carried out using 48 high-lean growth pigs from two different growth phases: from 15 to 30 kg (phase 1) and from 30 to 60 kg (phase 2). Pigs from growth phase 1 were fed with three different levels of dietary aP (0.107%, 0.321% or 0.535%) and submitted either to a thermoneutral (24°C and RH at 76%) or to a heat stress (34°C and RH at 70%) environment. Pigs from growth phase 2 were fed with three different levels of dietary aP (0.116%, 0.306% or 0.496%) and submitted either to a thermoneutral (22ºC and RH at 77%) or to a heat stress (32ºC and RH at 73%) environment. Heat stress decreased (P < 0.001) average daily feed i ntake at both growth phases. At 24°C, pigs in phase 1 fed the 0.321% aP diet had greater average daily gain and feed conversion (P < 0.05) than those fed the 0.107% or 0.535% while, at 34°C pigs fed the 0.535% aP had the best performance (P < 0.05). Pigs from phase 2 fed the 0.306% aP had best performance in both thermal environments. Gene expression profile was analyzed by quantitative real-time polymerase chain reaction. Irrespective of growing phase, the expression of six genes was lower (P < 0.05) at high temperature than at thermoneutrality. The lower expression of these genes under high temperatures evidences the effects of heat stress by decreasing oxidative metabolism, through adaptive physiological mechanisms in order to reduce heat production. In pigs from phase 1, six genes were differentially expressed across aP levels (P < 0.05) in the thermoneutral and one gene in the heat stress. In pigs from phase 2, two genes were differentially expressed across aP levels (P < 0.05) in both thermal environments. These data revealed strong evidence that phosphorus and thermal environments are key factors to regulate oxidative phosphorylation with direct implications on animal performance.Keywords: nutrigenomics, oxidative phosphorylation, pig production, heat stress and animal production, phosphorous levels for pig nutrition ImplicationsData from this study are the first to reveal the effects of different available phosphorus (P) levels in Longissimus dorsi gene expression related to oxidative phosphorylation and its implications on pig performance. Moreover, this study showed the effects of high temperatures on the expression of these genes and how this may alter the physiological response to P. This information will contribute to better understand the role of P in energy metabolism and will bring new insights into the comprehension of the effects of high temperatures on nutritional requirements and mitochondrial function.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Weller

Alebrante

Campos

et al. 2013

Animal

View full text Add to dashboard Cite

show abstract

“…The genome contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA) and 1 non-coding region. The components and their arrangements are the same as for other Canids (Arnason et al, 2006) and other mammals (Xu et al, 1996;Lin et al, 1999;Gu et al, 2007;Hiendleder et al, 2008).…”

Section: Characteristics Of the Tibetan Mastiff Mitochondrial Genomementioning

confidence: 99%

Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae)

Zhao

et al. 2011

Animal

View full text Add to dashboard Cite

In this study, the complete sequence of the Tibetan Mastiff mitochondrial genome (mtDNA) was determined, and the phylogenetic relationships between the Tibetan Mastiff and other species of Canidae were analyzed using the coyote (Canis latrans) as an outgroup. The complete nucleotide sequence of the Tibetan Mastiff mtDNA was 16 710 bp, and included 22 tRNA genes, 2S rRNA gene, 13 protein-coding genes and one non-coding region (D-loop region), which is similar to other mammalian mitochondrial genomes. The characteristics of the protein-coding genes, non-coding region, tRNA and rRNA genes among Canidae were analyzed in detail. Neighbor-joining and maximum-parsimony trees of Canids constructed using 12 mitochondrial protein-coding genes showed that as the coyotes and Tibetan wolves clustered together, so too did the gray wolves and domestic dogs, suggesting that the Tibetan Mastiff originated from the gray wolf as did other domestic dogs. Domestic dogs clustered into four clades, implying at least four maternal origins (A to D). The Tibetan Mastiff, which belongs to clade A, appears to be closely related to the Saint Bernard and the Old English Sheepdog.

show abstract

“…The fragment coding for 28S presents regions variables, some of which are significantly different in size between diverse animal species [6,7,8,9,10,11].…”

Section: Discussionmentioning

confidence: 99%

A suspected case of hunting accident. Case report

Procaccianti¹,

Pascali²,

Triolo³

et al. 2010

J Forensic Res

View full text Add to dashboard Cite

Nowadays, the use of molecular biology in forensics has made it possible to identify human victim and sometimes even the circumstances under which the death occurred through. In our case, a corpse of a 50-years old man with a gunshot wound was found in the woods.The suspected murderer declared that it had been a hunting accident while he shot a wild boar. During the autopsy, a bullet (Borra-bullet Gualandi, 32 gr) was found in the abdomen of the victim.

show abstract

Complete nucleotide sequence of pig (Sus scrofa) mitochondrial genome and dating evolutionary divergence within Artiodactyla

Cited by 72 publications

References 23 publications

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae)

A suspected case of hunting accident. Case report

Contact Info

Product

Resources

About