Complete nucleotide sequence of filamentous phage Cf1c from<i>Xanthomonas campestris</i>pv.<i>citri</i>

Kuo, Tsong‐Teh; Tan, Mian‐Shin; Su, M.; Yang, Ming‐Der

doi:10.1093/nar/19.9.2498

Cited by 41 publications

(20 citation statements)

References 6 publications

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“…The DNA sequence of VGJ also revealed the presence of two sites homologous to att sequences known to function in integrative filamentous phages, such as CTX of V. cholerae, f237 of V. parahaemolyticus, and the Cf1c, Cf16-v1, and Lf phages of X. campestris (10,24,25,36,37). The att-like sites in VGJ were found to overlap in opposite directions ( Fig.…”

Section: Resultsmentioning

confidence: 98%

VGJφ, a Novel Filamentous Phage of Vibrio cholerae , Integrates into the Same Chromosomal Site as CTXφ

et al. 2003

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We describe a novel filamentous phage, designated VGJ, isolated from strain SG25-1 of Vibrio cholerae O139, which infects all O1 (classical and El Tor) and O139 strains tested. The sequence of the 7,542 nucleotides of the phage genome reveals that VGJ has a distinctive region of 775 nucleotides and a conserved region with an overall genomic organization similar to that of previously characterized filamentous phages, such as CTX of V. cholerae and Ff phages of Escherichia coli. The conserved region carries 10 open reading frames (ORFs) coding for products homologous to previously reported peptides of other filamentous phages, and the distinctive region carries one ORF whose product is not homologous to any known peptide. VGJ, like other filamentous phages, uses a type IV pilus to infect V. cholerae; in this case, the pilus is the mannose-sensitive hemagglutinin. VGJ-infected V. cholerae overexpresses the product of one ORF of the phage (ORF112), which is similar to single-stranded DNA binding proteins of other filamentous phages. Once inside a cell, VGJ is able to integrate its genome into the same chromosomal attB site as CTX, entering into a lysogenic state. Additionally, we found an attP structure in VGJ, which is also conserved in several lysogenic filamentous phages from different bacterial hosts. Finally, since different filamentous phages seem to integrate into the bacterial dif locus by a general mechanism, we propose a model in which repeated integration events with different phages might have contributed to the evolution of the CTX chromosomal region in V. cholerae El Tor.

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Section: Resultsmentioning

confidence: 98%

VGJφ, a Novel Filamentous Phage of Vibrio cholerae , Integrates into the Same Chromosomal Site as CTXφ

et al. 2003

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“…This is the case of the filamentous phage Cf1 that has been commonly found infecting Xanthomonas species. This phage has the ability to reduce the growth of infected cells (67). While several phages have been identified in X. fastidiosa strains 9a5c, 6c, and 32 (X. fastidiosa subsp.…”

Section: Discussionmentioning

confidence: 99%

New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination

Jacques

Denancé

Legendre³

et al. 2016

Appl Environ Microbiol

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Xylella fastidiosa is a xylem-limited phytopathogenic bacterium endemic to the Americas that has recently emerged in Asia and Europe. Although this bacterium is classified as a quarantine organism in the European Union, importation of plant material from contaminated areas and latent infection in asymptomatic plants have engendered its inevitable introduction. In 2012, four coffee plants (Coffea arabica and Coffea canephora) with leaf scorch symptoms growing in a confined greenhouse were detected and intercepted in France. After identification of the causal agent, this outbreak was eradicated. Three X. fastidiosa strains were isolated from these plants, confirming a preliminary identification based on immunology. The strains were characterized by multiplex PCR and by multilocus sequence analysis/typing (MLSA-MLST) based on seven housekeeping genes. One strain, CFBP 8073, isolated from C. canephora imported from Mexico, was assigned to X. fastidiosa subsp. fastidiosa/X. fastidiosa subsp. sandyi. This strain harbors a novel sequence type (ST) with novel alleles at two loci. The two other strains, CFBP 8072 and CFBP 8074, isolated from Coffea arabica imported from Ecuador, were allocated to X. fastidiosa subsp. pauca. These two strains shared a novel ST with novel alleles at two loci. These MLST profiles showed evidence of recombination events. We provide genome sequences for CFBP 8072 and CFBP 8073 strains. Comparative genomic analyses of these two genome sequences with publicly available X. fastidiosa genomes, including the Italian strain CoDiRO, confirmed these phylogenetic positions and provided candidate alleles for coffee plant adaptation. This study demonstrates the global diversity of X. fastidiosa and highlights the diversity of strains isolated from coffee plants.

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“…In those experiments, both attP and attB sequences were determined. Other filamentous Fftype phages known to have a lysogenic cycle include X. campestris phages Cf1c (Kuo et al, 1991), Cf1t (Kuo et al, 1987a, b), Cf16v1 (Dai et al, 1988) and wLf (Lin et al, 2001), Xylella fastidiosa phage Kfw f1 (Simpson et al, 2000), Yersinia pestis phage CUSw-2 (Gonzalez et al, 2002), and Vibrio cholerae phages VGJw (Campos et al, 2003) and CTXw (Huber & Waldor, 2002). These host bacteria are pathogenic for plants and animals, and the phages are frequently involved in this pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

New bacteriophages that infect the phytopathogen Ralstonia solanacearum

et al. 2007

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Four kinds of bacteriophage (wRSL, wRSA, wRSM and wRSS) were isolated from Ralstonia solanacearum, a soil-borne Gram-negative bacterium that is the causative agent of bacterial wilt in many important crops. The Myovirus-type phages wRSL1 and wRSA1 contained dsDNA genomes of 240 kbp and 39 kbp, respectively. These phages have relatively wide host ranges and gave large clear plaques with various host strains; especially wRSA1 was able to infect all 15 R. solanacearum strains of different races or different biovars tested in this study. Three host strains contained wRSA1-related sequences in their genomic DNAs, suggesting a lysogenic cycle of wRSA1. Two phages, wRSM1 and wRSS1, were characterized as Ff-type phages (Inovirus) based on their particle morphology, genomic ssDNA and infection cycle. However, despite their similar fibrous morphology, their genome size (9.0 kb for wRSM1 and 6.6 kb for wRSS1) and genome sequence were different. Strains of R. solanacearum that were sensitive to wRSM1 were resistant to wRSS1 and vice versa. Several R. solanacearum strains contained wRSM1-related sequences and at least one strain produced wRSM1 particles, indicating the lysogenic state of this phage. These phages may be useful as a tool not only for molecular biological studies of R. solanacearum pathogenicity but also for specific and efficient detection (wRSM1 and wRSS1) and control of harmful pathogens (wRSL and wRSA) in cropping ecosystems as well as growing crops.

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Complete nucleotide sequence of filamentous phage Cf1c fromXanthomonas campestrispv.citri

Cited by 41 publications

References 6 publications

VGJφ, a Novel Filamentous Phage of Vibrio cholerae , Integrates into the Same Chromosomal Site as CTXφ

VGJφ, a Novel Filamentous Phage of Vibrio cholerae , Integrates into the Same Chromosomal Site as CTXφ

New Coffee Plant-Infecting Xylella fastidiosa Variants Derived via Homologous Recombination

New bacteriophages that infect the phytopathogen Ralstonia solanacearum

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