Complete Nucleotide Sequence of Carbazole/Dioxin-degrading Plasmid pCAR1 in Pseudomonas resinovorans Strain CA10 Indicates its Mosaicity and the Presence of Large Catabolic Transposon Tn4676

Maeda, Kaoru; Nojiri, Hideaki; Shintani, Masaki; Yoshida, Takako; Habe, Hiroshi; Omori, Toshio

doi:10.1016/s0022-2836(02)01400-6

Cited by 133 publications

(118 citation statements)

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“…The products of genes for the Mpf system of these two Inc groups show similarities to each other (51). On the other hand, IncP-7 plasmids carry the equivalent gene sets (tra for Dtr, and trh for Mpf, note that trhF, traF, and traD were renamed trhP, trhF, and traG, 129), although they show more similarity to the systems found from the plasmids of Enterobacteriaceae than to those of IncP-1 or IncP-9 plasmids (56,129). As for the Dtr system, Garcillán-Barcia and coworkers proposed six families of MOB based on a classification of the essential protein for transfer machinery, relaxases (38).…”

Section: Introductionmentioning

confidence: 91%

“…Interestingly, the Dtr systems of the three Inc group plasmids belong to distinct families; IncP-1 (TraI) to MOBP, IncP-7 (TraI) to MOB H , and IncP-9 (TraC) to MOB F (38). The location of the oriT of IncP-1 and IncP-9 plasmids has already been determined experimentally (39,81), whereas that of IncP-7 is still not clear, although the putative oriT region was suggested (56,129). The transcriptional regulation of genes for conjugative transfer on IncP-1 plasmids has been a Substrates of the host of each degradative plasmid: pADP-1, atrazine; pBS265, pBS267, pBS268, ε-caprolactam; pBRC60, pEST4011, pIJB1, pJP4, 2,4-Dichlorophenoxyacetic acid; NAH7, NPL-1, p8C, p15C, pBS240, pBS243, pBS1141, pBS1181, pBS1191, pC1, pDTG1, pNK33, pNK43, pNF143, pOS18, pOS19, pFME4, pFME5, pAK5, pNAH20, pNL4, pNL22, pNL25, pNL29, pNL31, pNL60, pOV17, pSN11, naphthalane, pA81, (halo)aromatic acid; pA8, chlorobenzoate; pSS60, 4-chlorobenzoate; pAC25, pENH91: 3-cholorobenzoate, pPS12-1, tetrachlorobenzene, pTSA, p-toluenesulfonic acid, pDK1, pSVS15, pWW0, pWW53, toluene/xylene, SAL1, salicylate, pCAR1, carbazole.…”

Section: Introductionmentioning

confidence: 98%

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The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in <i>Pseudomonas</i> within Natural Environments and Microcosms

et al. 2010

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Over the past few decades, degradative plasmids have been isolated from bacteria capable of degrading a variety of both natural and man-made compounds. Degradative plasmids belonging to three incompatibility (Inc) groups in Pseudomonas (IncP-1, P-7, and P-9) have been well studied in terms of their replication, maintenance, and capacity for conjugative transfer. The host ranges of these plasmids are determined by replication or conjugative transfer systems. The host range of IncP-1 is broad, that of IncP-9 is intermediate, and that of IncP-7 is narrow. To understand the behavior of these plasmids and their hosts in various environments, the survivability of inocula, stability or transferability, and efficiency of biodegradation in environments and microcosms have been monitored. The biodegradation and plasmid transfer in various environments have been observed for all three groups, although the kinds of transconjugants differed with the Inc groups. In some cases, the deletion and amplification of catabolic genes acted to reduce the production of toxic catabolic intermediates, or to increase the activity on a particular catabolic pathway. The combination of degradative genes, the plasmid backbone of each Inc group, and the host of the plasmids is key to the degraders adapting to various hosts or to heterogeneous environments.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 98%

The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in <i>Pseudomonas</i> within Natural Environments and Microcosms

et al. 2010

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“…Cell suspensions of Pseudomonas pseudoalcaligenes strain POB310 cooxidized a DE analogue, 4-carboxybenzophenone, yielding the corresponding angular diol 36,39) . Biodegradation of CAR, a nitrogen-containing DF analogue, via angular dioxygenation has been intensively studied in Pseudomonas resinovorans strain CA10 62,63,117,129,135,137,142,158,159,194) and some other species of Proteobacteria 53,58,84,106,141,142,165) . The metabolic pathway proposed for CAR in Pseudomonas resinovorans strain CA10 is homologous to the degradation route for DF which is initiated by angular dioxygenase attack.…”

Section: Biodegradation Via Angular Dioxygenationmentioning

confidence: 99%

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Hiraishi

2003

Microb. Environ.

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Dioxins are a group of chloroaromatic compounds known to be toxic and carcinogenic, and persistent environmental pollutants. How to remedy dioxin-polluted environments is one of the most challenging problems in environmental technology. From ecological and economical viewpoints, biological methods using particular microorganisms and microbial consortia capable of dioxin transformation and degradation have greater appeal than physicochemical methods in their application for environmental remediation. Large numbers of microorganisms capable of degrading dioxins and dioxin-like compounds have been isolated and characterized. Information about the biodiversity, ecophysiology and molecular biology of dioxin-degrading microorganisms has accumulated particularly during the past decade. There are three major modes of microbial degradation and transformation of dioxins; that is, oxidative degradation by aerobic bacteria containing aromatic hydrocarbon dioxygenases, reductive dechlorination by anaerobic microorganisms and fungal oxidation with cytochrome P-450, lignin peroxidases and laccases. This article overviews the current knowledge of microbial degradation and transformation of dioxins as well as the biodiversity of microorganisms involved. Strategies directed towards the bioremediation of dioxin-polluted environments are discussed.

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“…In addition, strains CA10, KA1, and IC177 have transposons, and strain CA10 also has the plasmid pCAR1. 7,31,32) These facts indicate that OC11 most likely obtained car OC11 through horizontal transmission using mobile DNA, such as transposons and plasmids. This speculation is also supported by the result that the car genes from strain OC11 showed high homology to those of strain IC177.…”

Section: Southern Hybridizationmentioning

confidence: 97%

Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, Janibacter sp. strain OC11

Oba

Suzuki

Maeda

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Strain OC11 was isolated from seawater sampled at the coast of Chiba, Japan, in artificial seawater medium with carbazole (CAR) as the sole carbon source. Its 16S ribosomal RNA gene sequence suggested that strain OC11 belongs to the genus Janibacter. The CAR-degradation genes (car genes) of strain OC11 were PCR amplified, using degenerate primers designed based on the car gene sequences of other CAR-degrading bacteria. Complete nucleotide sequences encoding six complete open reading frames were determined, and the first known ferredoxin reductase gene (carAd) was found from a CAR-degrading bacterium isolated from the marine environment. An experiment using a mutant strain suggested that the car genes of strain OC11 are functional in CAR degradation. Southern hybridization indicated that strain OC11 had one car gene cluster in vivo. RT-PCR revealed that transcription of carOC11 constitutes an operon.

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Complete Nucleotide Sequence of Carbazole/Dioxin-degrading Plasmid pCAR1 in Pseudomonas resinovorans Strain CA10 Indicates its Mosaicity and the Presence of Large Catabolic Transposon Tn4676

Cited by 133 publications

References 62 publications

The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in <i>Pseudomonas</i> within Natural Environments and Microcosms

The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in <i>Pseudomonas</i> within Natural Environments and Microcosms

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Characterization and genetic analyses of a carbazole-degrading gram-positive marine isolate, Janibacter sp. strain OC11

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