Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type

Locali, E. C.; Freitas-Astúa, Juliana; Souza, Alessandra Alves de; Takita, Marco Aurélio; Astúa-Monge, Gustavo; Antonioli-Luizon, R.; Rodrigues, Vandeclei; Targon, M. L. P. N.; Machado, Marcos Antônio

doi:10.1099/vir.0.82038-0

Cited by 103 publications

(88 citation statements)

References 8 publications

(16 reference statements)

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“…The expressed protein had a molecular mass of ca. 35 kDa, close to the value of 32.5 kDa estimated by Locali-Fabris et al (2006) for the CiLV-C putative movement protein. The difference of 2.5 kDa on the in vitro MP mass is due to the six histidine tag of the recombinant MP.…”

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confidence: 58%

“…The 286 kDa protein is a polyprotein putatively involved in virus replication, and the 29 kDa protein is the putative coat protein of CiLV-C. RNA 2 (4986 nt) contains four ORFs which potentially encode, respectively, 15, 61, 32 and 24 kDa proteins. Among these, only the 32 kDa protein, encoded by ORF 3, has a putative function assigned, apparently being involved in cell-to-cell movement of the virus (Locali-Fabris et al, 2006;Pascon et al, 2006).…”

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confidence: 99%

“…Furthermore, it can lead to heavy losses due to the drop of leaves and fruits and, eventually, the premature death of the trees (Bastianel et al, 2010). CiLV-C, recently classified as the type species of a new genus of plant viruses, Cilevirus (Carstens, 2010), has a bipartite genome composed by two ssRNA molecules of positive polarity (Locali-Fabris et al, 2006;Pascon et al, 2006). RNA 1 (8745 nucleotides -nt) contains two open reading frames (ORFs), which potentially encode 286 and 29 kDa proteins.…”

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confidence: 99%

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In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C)

Calegario¹,

Labate²,

Peroni³

et al. 2012

Trop. plant pathol.

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Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter.

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confidence: 58%

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confidence: 99%

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confidence: 99%

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In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C)

Calegario¹,

Labate²,

Peroni³

et al. 2012

Trop. plant pathol.

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“…The complete sequence of the genome of CiLV-C was obtained (Pascon et al, 2006) being a bipartite positive sense ssRNA distinct from known viruses. As a consequence, a new genus, Cilevirus, was proposed for this virus (Locali-Fabris et al, 2006).…”

Section: Plant Viruses Transmitted By Brevipalpus Mitesmentioning

confidence: 99%

An annotated list of ornamentals naturally found infected by Brevipalpus mite-transmitted viruses

Kitajima¹,

Rodrigues²,

Freitas-Astúa

2010

Sci. agric. (Piracicaba, Braz.)

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The first cases of ornamental plants found infected by Brevipalpus transmitted viruses (BTV) were described in the 1990's from the region of Piracicaba, State of São Paulo, Brazil; subsequent cases were from other regions in the country and other American countries. Currently, 37 ornamental plant species (for the sake of simplicity, orchids being considered as a single species), belonging to 18 families of dicotyledons, have been reported hosting BTV. Because of the non systemic type of infection of these viruses, the localized diseases they cause are unimportant usually, but they have the potential to cause economic losses if severe outbreaks of Brevipalpus mite populations occur. Some ornamentals may serve as reservoirs to BTV known to cause serious damage to food crops as Citrus leprosis virus-cytoplasmic type (CiLV-C), passion fruit green spot virus (PFGSV) and Coffee ringspot virus (CoRSV). Key words: Acari, Tenuipalpidae, ornamental industry Lista comentada de plantas ornamentais naturalmente infectadas por vírus transmitidos por ácaros Brevipalpus RESUMO: Os primeiros casos de plantas ornamentais encontradas naturalmente infetadas por vírus transmitidos por Brevipalpus (Acari: Tenuipalpidae) (VTB) foram registrados nos anos 1990 na região de Piracicaba, Estado de São Paulo, e ocorrências subseqüentes foram observadas em várias outras regiões do país e de outros países das Américas. Atualmente acham-se relatadas 37 espécies de ornamentais (para efeito de simplificação, orquídeas foram consideradas como única espécie) pertencentes a 18 famílias botânicas. Pelo fato de causarem apenas infecções localizadas, geralmente nas folhas, VTB em ornamentais não causam preocupações aos produtores, mas potencialmente podem causar perdas econômicas se ocorrerem explosões populacionais do ácaro vetor. Plantas ornamentais podem servir de reservatório de VTB de importância econômica como os vírus da leprose dos citros-tipo citoplasmático (CiLV-C), da mancha verde do maracujá (PFGSV) e da mancha anular do cafeeiro (CoRSV). Palavras-chave: Acari, Tenuipalpidae, indústria de ornamentais BREVIPALPUS MITES

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“…It was also found that leprosis symptoms may be caused by two distinct viruses based on the cytopathic effects Rodrigues et al, 2003), both transmitted by Brevipalpus mites: the rare nuclear type (Citrus leprosis virus N-CiLV-N), a likely member of the Dichorhabdovirus genus, whose type member is Orchid fleck virus (OFV) (Kondo et al, 2006); and the prevalent and more aggressive cytoplasmic type Citrus leprosis virus C (CiLV-C). Its genome was entirely sequenced (Locali-Fabris et al, 2006;Pascon et al, 2006) and it was accepted as the type-member Present status of citrus leprosis in Argentina and Paraguay of the new genus Cilevirus (Locali-Fabris et al, 2006;Carstens, 2010;Locali-Fabris et al, 2012), resulting in molecular (Locali et al, 2003) and immunochemical tools (Calegario et al, 2013) for CiLV-C detection. Although symptoms in the fruits and barks caused by CiLV-C and CiLV-N are undistinguishable, there is a slight but consistent difference in leaf symptoms: CiLV-N causes smaller lesions with a dark central spot surrounded by a bright yellow halo while lesions caused by CiLV-C may reach larger size and present a light green color commonly with one or more gummy rings (Bastianel et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Present status of citrus leprosis in Argentina and Paraguay

Cáceres¹,

Aguirre²,

Costa³

et al. 2013

Trop. plant pathol.

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Citrus leprosis (CL) was first described in South America in the 1920's. It is considered similar to a disease first observed back to 1860 in Florida. It is a destructive disease characterized by localized lesions on the leaves, fruits and stems, which may lead to the death of the affected plant if left untreated. Around 1940, CL was demonstrated to be transmitted by Brevipalpus mites in Argentina. Because little information exists on the status of CL pathosystem in Argentina and Paraguay, a survey was made in several citrus growing areas of these countries from 2009 to 2011, to evaluate its presence and relevance as well as the identification of the virus and the mite vector. CL was found in most of the sweet orange and/or mandarin orchards in Paraguay (Departamentos de Boquerón, Concepción, San Pedro, Cordillera, Alto Paraná, Itapúa) and Argentina (Provincias de Misiones, Corrientes, Entre Ríos). Incidence was usually low. The causal virus was identified as Citrus leprosis virus C (CiLV-C) by RT-PCR, electron microscopy and immunofluorescence. In all the visited regions in Paraguay and the region of Montecarlo, Argentina, the mites collected in plants infected by CiLV-C were identified as B. phoenicis.

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Complete nucleotide sequence, genomic organization and phylogenetic analysis of Citrus leprosis virus cytoplasmic type

Cited by 103 publications

References 8 publications

In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C)

In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C)

An annotated list of ornamentals naturally found infected by Brevipalpus mite-transmitted viruses

Present status of citrus leprosis in Argentina and Paraguay

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