Complete nucleotide sequence and experimental host range of Okra mosaic virus

Stephan, D.; Siddiqua, Mahbuba; Hoang, Anh Ta; Engelmann, J.; Winter, Stephan; Maiß, E.

doi:10.1007/s11262-007-0181-1

Cited by 18 publications

(9 citation statements)

References 44 publications

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“…The PathoChip screen also provided some surprising results. For example, detection of the sequences related to Okra mosaic virus 39 , and citrus viroid V ( Supplementary Fig. S1, Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

Distinct microbiological signatures associated with triple negative breast cancer

Banerjee

Wei

Tan

et al. 2015

Sci Rep

102

154

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Infectious agents are the third highest human cancer risk factor and may have a greater role in the origin and/or progression of cancers, and related pathogenesis. Thus, knowing the specific viruses and microbial agents associated with a cancer type may provide insights into cause, diagnosis and treatment. We utilized a pan-pathogen array technology to identify the microbial signatures associated with triple negative breast cancer (TNBC). This technology detects low copy number and fragmented genomes extracted from formalin-fixed paraffin embedded archival tissues. The results, validated by PCR and sequencing, define a microbial signature present in TNBC tissue which was underrepresented in normal tissue. Hierarchical clustering analysis displayed two broad microbial signatures, one prevalent in bacteria and parasites and one prevalent in viruses. These signatures demonstrate a new paradigm in our understanding of the link between microorganisms and cancer, as causative or commensal in the tumor microenvironment and provide new diagnostic potential.

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Section: Discussionmentioning

confidence: 99%

Distinct microbiological signatures associated with triple negative breast cancer

Banerjee

Wei

Tan

et al. 2015

Sci Rep

102

154

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“…These plant diseases cause significant crop losses and economic burden each year. For example, in the case of Okra mosaic virus ( Tymovirus ), early infection of okra leads to yield losses of 12%–90% depending on the severity of the disease [20]. MRFV ( Marafivirus ) is of great agronomic importance, as it produces significant yield losses in maize throughout Central and South America [21].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of a Novel Virus of the Family Tymoviridae Isolated from Mosquitoes

Wang¹,

Lv²,

Zhai³

et al. 2012

PLoS ONE

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BackgroundThe family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV.Methods and ResultsThe CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called “tymobox” or “marafibox”, the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.ConclusionsThe CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.

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“…As most tymoviruses, ChiYMV has a narrow experimental host range, with a higher susceptibility of species from the family of the natural host [36][37][38]. No local lesión host was identified.…”

Section: Discussionmentioning

confidence: 99%

Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

et al. 2010

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The characterization of viruses infecting wild plants is a key step towards understanding the ecology of plant viruses. In this work, the complete genomic nucleotide sequence of a new tymovirus species infecting chiltepin, the wild ancestor of Capsicum annuum pepper crops, in México was determined, and its host range has been explored. The genome of 6,517 nucleotides has the three open reading frames described for tymoviruses, putatively encoding an RNA-dependent RNA polymerase, a movement protein and a coat protein. The 5' and 3' untranslated regions have structures with typical signatures of the tymoviruses. Phylogenetic analyses revealed that this new virus is closely related to the other tymoviruses isolated from solanaceous plants. Its host range is mainly limited to solanaceous species, which notably include cultivated Capsicum species. In the latter, infection resulted in a severe reduction of growth, indicating the potential of this virus to be a significant crop pathogen. The ñame of chiltepin yellow mosaic virus (ChiYMV) is proposed for this new tymovirus.

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Complete nucleotide sequence and experimental host range of Okra mosaic virus

Cited by 18 publications

References 44 publications

Distinct microbiological signatures associated with triple negative breast cancer

Distinct microbiological signatures associated with triple negative breast cancer

Genomic Characterization of a Novel Virus of the Family Tymoviridae Isolated from Mosquitoes

Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

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