Complete mitochondrial genomes of two blattid cockroaches, Periplaneta australasiae and Neostylopyga rhombifolia, and phylogenetic relationships within the Blattaria

Abstract

Complete mitochondrial genomes (mitogenomes) of two cockroach species, Periplaneta australasiae and Neostylopyga rhombifolia, 15,605 bp and 15,711 bp in length, respectively, were determined. As reported for other cockroach mitogenomes, the two mitogenomes possessed typical ancestral insect mitogenome gene composition and arrangement. Only several small intergenic spacers were found: one, which was common in all sequenced cockroach mitogenomes except for the genus Cryptocercus, was between tRNA-Ser (UCN) and ND1 and contained a 7bp highly conserved motif (WACTTAA). Three different types of short tandem repeats in the N. rhombifolia control region (CR) were observed. The homologous alignments of these tandem repeats with other six cockroach mitogenome CRs revealed a low similarity. Three conserved sequence blocks (CSB) were detected in both cockroach mitochondrial CRs. CSB1 was specific for blattinine mitogenomes and was highly conserved with 95% similarity, speculating that this block was a possible molecular synapomorphy for this subfamily. CSB3 located nearby downstream of CSB1 and has more variations within blattinine mitogenomes compared with CSB1. The CSB3 was capable of forming stable stem-loop structure with a small T-stretch in the loop portion. We assessed the influence of four datasets and two inference methods on topology within Orthopteroidea. All genes excluding the third codon positions of PCGs could generate more stable topology, and higher posterior probabilities than bootstrap values were presented at some branch nodes. The phylogenetic analysis with different datasets and analytical methods supported the monophyly of Dictyoptera and supported strongly the proposal that Isoptera should be classified as a family (Termitidae) of the Blattaria. Specifically, Shelfordella lateralis was inserted in the clade Periplaneta. Considering the K2P genetic distance, morphological characters, and the phylogenetic trees, we suggested that S. lateralis should be placed in the genus Periplaneta.

Phylogeny and age of cockroaches: a reanalysis of mitogenomes with selective fossil calibrations

“…The DNA data in the GenBank files were extracted using PHYLOSUITE. Most of the mitogenomes (68.4%) are from Bourguignon et al (2018), and the remaining from Yamauchi et al (2004), Cameron et al (2005Cameron et al ( , 2012, Zhang YY et al (2009), Kômoto et al (2010Kômoto et al ( , 2012, Chen (2012), Wang et al (2014), Jeon and Park (2015), Tian et al (2015), Cheng et al (2016), Ye et al (2016), Chen et al (2017), Dumans et al (2017), Ma et al (2017), Gong et al (2018), andZhang LP et al (2018).…”

Complete mitochondrial genome of Episymploce splendens (Blattodea: Ectobiidae): A large intergenic spacer and lacking of two tRNA genes

“…There are of 4,600 species of cockroaches [ 16 ] and 300 mitochondrial genomes of them have been sequenced and uploaded to the NCBI database. The blattarian mito-genomes seem to be conserved in evolution [ 16 , 17 ]. The gene rearrangement was only reported in Cryptocercidae within blattarian mito-genomes [ 4 ].…”