Complete mitochondrial genome sequence of the food fermentation fungus, Aspergillus luchuensis

Abstract

Aspergillus luchuensis is a filamentous fungus used for food and alcohol fermentation in many Asian countries. Here, we reported the complete mitochondrial genome sequence of A. luchuensis isolated from fermented soybean brick, called as Meju, in Korea. Its mitochondrial genome was successfully assembled from raw reads sequenced using MiSeq by Velvet and GapCloser. Total length of the mitochondrial genome is 31,228 bp and encoded 44 genes (16 protein-coding genes, two rRNAs, and 26 tRNAs). Nucleotide sequence of coding region takes over 25.6%, and overall GC content is 26.4%. Phylogenetic tree of mitochondrial genomes presented A. luchuensis and Aspergillus kawachii are clustered in one clade. This mitochondrial genome can be used for further analyses of Aspergillus mitochondrial comparative genomics to improve understanding of diverse Aspergillus species. ARTICLE HISTORY

Complete mitochondrial genome sequence of Afla-Guard®, commercially available non-toxigenic Aspergillus flavus

“… 2019 ; Park, Kwon, Zhu, Mageswari, Heo, Han, et al. 2019 ; Park, Kwon, Zhu, Mageswari, Heo, Kim, et al. 2019 ) including that of Afla-Guard ® and one Podospora mitochondrial genome (Cummings et al.…”

Complete mitochondrial genome sequence of an aflatoxin B and G producing fungus, Aspergillus parasiticus

“…Raw data generated by MISeq were subject to de novo assembly done by Velvet 1.2.10 (Zerbino and Birney 2008) and gap filling with SOAPGapCloser 1.12 (Zhao et al 2011) to get complete mitochondrial genome after confirming each bases using BWA 0.7.10 and SAMtools 1.9 (Li et al 2009;Li 2013). Geneious R11 11.0.5 (Biomatters Ltd, Auckland, New Zealand) was used to annotate its chloroplast genome by comparing with that of Aspergillus luchuensis (MK061298; Park et al 2019). Voucher sample was deposited into KACC with accession KACC-46475.…”

“…The length of A. parasiticus mitochondrial genome (Genbank accession is MK124769) is 29,141 bp, which is shortest length among nine available Aspergillus mitochondrial genomes (Futagami et al 2011;Joardar et al 2012;Xu et al 2018) (Park et al 2019). A. parasiticus COX1 gene, which contains several introns on fungal mitochondrial genomes (Joardar et al 2012) has one intron sequence.…”

Complete mitochondrial genome sequence of a xerophilic fungus, Aspergillus pseudoglaucus

“…It has been isolated from seafoods and soil (Smetanina et al 2007;S eguin et al 2014). It has also been used as a start culture for Kastuobushi in Japan (Pitt and Hocking 2009) and frequently identified from Meju, a soybean brick used for making soy source and soybean paste in Korea (Hong et al 2011). It can produce various metabolites such as benzyl derivatives binding to human opioid or cannabinoid receptors (Gao et al 2011), mycophenolic acid (Mouhamadou et al 2017), and various antibacterial and antifungal compounds (Gao et al 2012).…”

“… 2009 ; Li 2013 ). Geneious R11 11.0.5 (Biomatters Ltd, Auckland, New Zealand) was used to annotate its mitochondrial genome by comparing with those of Aspergillus luchuensis (MK061298; Park, Kwon, Zhu, Mageswari, Heo, Han, Hong, under review ) and Aspergillus parasiticus (MK124769; Park et al under review ). Voucher sample was deposited into Korean Agricultural Culture Collection (KACC; Republic of Korea; http://genebank.rda.go.kr/ ; KACC-93211).…”