Complete Mitochondrial Genome Sequence of
            <i>Aethina tumida</i>
            (Coleoptera: Nitidulidae), a Beekeeping Pest

Duquesne, Véronique; Delcont, Aurélie; Huleux, Anthéa; Beven, Véronique; Touzain, Fabrice; Ribière‐Chabert, Magali

doi:10.1128/genomea.01165-17

Cited by 4 publications

(3 citation statements)

References 7 publications

(8 reference statements)

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“…Genomic DNA isolated from one beetle was sequenced using Illumina's MiSeq platform (Illumina). The complete mitochondrial genome of the A. tumida was used as a reference sequence (Duquesne et al 2017). The resultant reads were assembled and annotated using the MITOS web server (Bernt et al 2013) and Geneious R9 (Kearse et al 2012).…”

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confidence: 99%

Complete mitochondrial DNA sequence of the small hive beetle Aethina tumida (Insecta: Coleoptera) from Hawaii

Takahashi

Okuyama

Martin

2019

Mitochondrial DNA Part B

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We analyzed the complete mitochondrial genome of the small hive beetle Aethina tumida, a major honeybee pest that was accidentally transported to the Hawaii Islands, USA. The mitochondrial genome of A. tumida was identified as a 15,198 bp circular molecule containing 13 protein-coding genes, 22 tRNA genes, and two rRNA genes, along with one A þ T-rich control region. The average AT content was found to be 76.2%. The heavy strand was predicted to have nine protein-coding and 14 tRNA genes; while the light strand was predicted to contain four protein-coding, eight tRNA, and two rRNA genes. Among the 13 PCGs, the initiation codon ATT was found in five, ATA in three, ATG in four, and TTG in one gene. Six PCGs used TAA as the stop codon, whereas three PCGs used TAG as the stop codon. Incomplete stop codon TA (COIII, ND5, and ND4) and T (COII) were identified. We found that the genetic distance between A. tumida from Hawaii and the beetles' natural home South Africa, was small, indicating that the Hawaii population may have originated for the South Africa region.

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confidence: 99%

Complete mitochondrial DNA sequence of the small hive beetle Aethina tumida (Insecta: Coleoptera) from Hawaii

Takahashi

Okuyama

Martin

2019

Mitochondrial DNA Part B

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“…The target sequence for A. tumida was a region of the mitochondrial genome sequence (accession number MF943248 [ 68 ]) that encompassed the cytochrome oxidase I gene (COI), that was analysed for a total of 139,780 BLAST hits. To detect the DNA of A. tumida , in addition to the novel PCR primer pair, we also tested the primers developed by Li et al [ 51 ] that were used for a qualitative assay.…”

Section: Methodsmentioning

confidence: 99%

Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to Identify Aethina tumida and Galleria mellonella Infestations

Ribani

Taurisano

Utzeri

2022

Veterinary Sciences

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Environmental DNA (eDNA) contained in honey derives from the organisms that directly and indirectly have been involved in the production process of this matrix and that have played a role in the hive ecosystems where the honey has been produced. In this study we set up PCR-based assays to detect the presence of DNA traces left in the honey by two damaging honey bee pests: the small hive beetle (Aethina tumida) and the greater wax moth (Galleria mellonella). DNA was extracted from 82 honey samples produced in Italy and amplified using two specific primer pairs that target the mitochondrial gene cytochrome oxidase I (COI) of A. tumida and two specific primer pairs that target the same gene in G. mellonella. The limit of detection was tested using sequential dilutions of the pest DNA. Only one honey sample produced in Calabria was positive for A. tumida whereas about 66% of all samples were positively amplified for G. mellonella. The use of honey eDNA could be important to establish early and effective measures to contain at the local (e.g., apiary) or regional scales these two damaging pests and, particularly for the small hive beetle, to prevent its widespread diffusion.

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“…The mitochondrial genome of insects has unique features, such as maternal inheritance, rapid evolution rate, stable gene composition, and high independence and integrity, making it a powerful genetic marker for studying the evolution of insects [45,46]. Previously, there were only seven complete mitochondrial genomes among three subfamilies of Nitidulidae in GenBank [29,[47][48][49]. Meligethinae, as the second largest subfamily of Nitidulidae, does not yet have a complete mitochondrial genome.…”

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confidence: 99%

The First Two Complete Mitochondrial Genomes for the Subfamily Meligethinae (Coleoptera: Nitidulidae) and Implications for the Higher Phylogeny of Nitidulidae

Dai,

Liu,

Di Giulio

et al. 2024

Insects

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The phylogenetic status of the family Nitidulidae and its sister group relationship remain controversial. Also, the status of the subfamily Meligethinae is not fully understood, and previous studies have been mainly based on morphology, molecular fragments, and biological habits, rather than the analysis of the complete mitochondrial genome. Up to now, there has been no complete mitochondrial genome report of Meligethinae. In this study, the complete mitochondrial genomes of Meligethinus tschungseni and Brassicogethes affinis (both from China) were provided, and they were compared with the existing complete mitochondrial genomes of Nitidulidae. The phylogenetic analysis among 20 species of Coleoptera was reconstructed via PhyloBayes analysis and Maximum likelihood (ML) analysis, respectively. The results showed that the full lengths of Meligethinus tschungseni and Brassicogethes affinis were 15,783 bp and 16,622 bp, and the AT contents were 77% and 76.7%, respectively. Each complete mitochondrial genome contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and a control region (A + T-rich region). All the PCGs begin with the standard start codon ATN (ATA, ATT, ATG, ATC). All the PCGs terminate with a complete terminal codon, TAA or TAG, except cox1, cox2, nad4, and nad5, which terminate with a single T. Furthermore, all the tRNAs have a typical clover-leaf secondary structure except trnS1, whose DHU arm is missing in both species. The two newly sequenced species have different numbers and lengths of tandem repeat regions in their control regions. Based on the genetic distance and Ka/Ks analysis, nad6 showed a higher variability and faster evolutionary rate. Based on the available complete mitochondrial genomes, the results showed that the four subfamilies (Nitidulinae, Meligethinae, Carpophilinae, Epuraeinae) of Nitidulidae formed a monophyletic group and further supported the sister group relationship of Nitidulidae + Kateretidae. In addition, the taxonomic status of Meligethinae and the sister group relationship between Meligethinae and Nitidulinae (the latter as currently circumscribed) were also preliminarily explored.

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Complete Mitochondrial Genome Sequence of Aethina tumida (Coleoptera: Nitidulidae), a Beekeeping Pest

Abstract: We report here the full mitochondrial genome sequence of Aethina tumida, a Nitidulidae species beetle, that is a pest of bee hives. The obtained sequence is 16,576 bp in length and contains 13 protein-coding genes, 2 rRNA genes, and 22 tRNAs.

Cited by 4 publications

References 7 publications

Complete mitochondrial DNA sequence of the small hive beetle Aethina tumida (Insecta: Coleoptera) from Hawaii

Complete mitochondrial DNA sequence of the small hive beetle Aethina tumida (Insecta: Coleoptera) from Hawaii

Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to Identify Aethina tumida and Galleria mellonella Infestations

The First Two Complete Mitochondrial Genomes for the Subfamily Meligethinae (Coleoptera: Nitidulidae) and Implications for the Higher Phylogeny of Nitidulidae

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