Complete mitochondrial genome of the Korean bitterling<i>Acheilognathus koreensis</i>(Cypriniformes; Cyprinidae)

Hwang, Dae‐Sik; Lee, Wan-Ok; Lee, Jae‐Seong

doi:10.3109/19401736.2013.766178

Cited by 8 publications

(5 citation statements)

References 8 publications

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“…All GC skews in the 13 PCGs ranged from ‒ 0.428 to ‒ 0.179 except for nad6 (0.423). This result suggests that more C nucleotides are present in most PCGs, and nad6 only presented negative AT and positive GC skews, which is consistent with most previous reports of strand asymmetry in freshwater fish (Hwang et al 2013b , c ).…”

Section: Resultssupporting

confidence: 91%

Characterization of the complete mitogenome of the endangered freshwater fish Gobiobotia naktongensis from the Geum River in South Korea: evidence of stream connection with the Paleo-Huanghe

Kim

Kang

Kim³

et al. 2022

Genes Genom

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Background The freshwater fish Gobiobotia naktongensis (Teleostei, Cypriniformes, and Gobionidae) is an endangered class I species whose population size has been greatly reduced. Objective To successfully protect and restore the highly endangered freshwater fish G. naktongensis from the Geum River in South Korea. Methods The mitogenome was characterized using the primer walking method with phylogenetic relationships. Results The complete mitogenome of G. naktongensis Geum River was 16,607 bp, comprising 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA (tRNA) genes. Seventeen substitutions were found by comparing the tRNA regions between G. naktongensis Geum and Nakdong Rivers and G. pappenheimi; most were specific to G. naktongensis Nakdong River, with changes in their secondary structures. The comparison between G. naktongensis Geum River and G. pappenheimi revealed differences in the lengths of the D-loop and two tRNAs (tRNAArg and tRNATrp) and the secondary structures in the TΨC-arm of tRNAHis. In the phylogenetic tree, G. naktongensis Geum River did not cluster with its conspecific specimen from the Nakdong River in South Korea, but showed the closest relationship to G. pappenheimi in mainland China. Conclusions Our results support the existence of the Paleo-Huanghe River connecting the Korean peninsula and mainland China, suggesting that G. naktongensis in the Geum River should be treated as a different evolutionarily significant unit separated from that in the Nakdong River. The complete mitogenome of G. naktongensis Geum River provides essential baseline data to establish strategies for its conservation and restoration.

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Section: Resultssupporting

confidence: 91%

Characterization of the complete mitogenome of the endangered freshwater fish Gobiobotia naktongensis from the Geum River in South Korea: evidence of stream connection with the Paleo-Huanghe

Kim

Kang

Kim³

et al. 2022

Genes Genom

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“…Kumgang fat minnow and the Korean oily bitterling belong to the order Cypriniformes, family Cyprinidae, mainly inhabit the upper reaches of the Han River and distribute in the rivers flowing into the South Sea or the West Sea toward the south of Geumgang. (Lee et al, 2008;Hwang et al, 2013). In this study, we isolated three novel strains (D17 T , D13, and D25 T ) during investigations of the gut mi-crobiota of the three fish using culture-dependent methods.…”

Section: Introductionmentioning

confidence: 93%

Description of Deefgea piscis sp. nov., and Deefgea tanakiae sp. nov., isolated from the gut of Korean indigenous fish

et al. 2022

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Three novel strains, (D17 T , D13, and D25 T ) isolated from the gut of the Korean dark sleeper ( Odontobutis platycephala ), Kumgang fat minnow ( Rhynchocypris kumgangensis ), and the Korean oily bitterling ( Tanakia koreensis ) were identified as two novel species. Strains D17 T and D13 showed the highest similarities in 16S rRNA gene and complete genome sequences to Deefgea rivuli WB 3.4–79 T (98.0% and 97.9%, respectively, of 16S rRNA gene sequence similarity, 77.8% and 77.7%, respectively, of orthologous average nucleotide identity, OrthoANI, and 21.9% and 21.9%, respectively, of digital DNA-DNA hybridization, dDDH). Strain D17 T showed the highest similarities in 16S rRNA gene and complete genome sequences to D13 (99.9% of 16S rRNA gene sequence similarity, 91.8% of OrthoANI, and 45.1% of dDDH); therefore, strains D17 T and D13 were assigned as the same species. Strain D25 T showed the highest similarities in 16S rRNA gene and complete genome sequences to D. chitinilytica Nsw-4 T (98.2% of 16S rRNA gene sequence similarity, 82.4% of OrthoANI, and 25.1% of dDDH). Strains D17 T and D13 were Gram-stain-negative, facultative anaerobes, rod-shaped, non-motile, and non-flagellated. Strain D25 T was Gram-stain-negative, facultative anaerobe, rod-shaped, and motile by a single polar flagellum. These strains had C 16:0 and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) as the major cellular fatty acids and possessed Q-8 as a major respiratory ubiquinone. All three strains contained phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. Based on polyphasic taxonomic data, strains D17 T , D13, and D25 T represent two novel species of the genus Deefgea. We propose the name Deefgea piscis sp. nov. for strains D17 T (= KCTC 82958 T = JCM 34941 T ) and D13 (= KCTC 92368), and Deefgea tanakiae sp. nov. for strain D25 T (= KCTC 82959 T = JCM 34942 T ). Supplemental material for this article may be found at 10.1007/s12275-022-2250-5.

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“…The laterally flattened spindle-shaped body of the oily bitterling is dark-brown on the dorsal side and light-brown on the ventral side. As part of a recent species preservation effort, genetic studies of Korean oily bitterling have been initiated; Hwang et al reported the complete mitochondrial genome sequence of A. koreensis [16]. Herein, we report the molecular cloning and characterization of the mlc2f gene of Korean oily bitterling ( Akmlc2f ) at the genomic DNA and mRNA levels.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Functional Analyses of the Fast Skeletal Myosin Light Chain2 Gene of the Korean Oily Bitterling, Acheilognathus koreensis

Kong

Lee

Kim

et al. 2013

IJMS

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We identified and characterized the primary structure of the Korean oily bitterling Acheilognathus koreensis fast skeletal myosin light chain 2 (Akmlc2f), gene. Encoded by seven exons spanning 3955 bp, the deduced 168-amino acid AkMLC2f polypeptide contained an EF-hand calcium-binding motif and showed strong homology (80%–98%) with the MLC2 proteins of Ictalurus punctatus and other species, including mammals. Akmlc2f mRNA was highly enriched in skeletal muscles, and was detectable in other tissues. The upstream regions of Akmlc2f included a TATA box, one copy of a putative MEF-2 binding site and several putative C/EBPβ binding sites. The functional activity of the promoter region of Akmlc2f was examined using luciferase and red fluorescent protein reporters. The Akmlc2f promoter-driven reporter expressions were detected and increased by the C/EBPβ transcription factor in HEK293T cells. The activity of the promoter of Akmlc2f was also confirmed in the developing zebrafish embryo. Although the detailed mechanism underlying the expression of Akmlc2f remains unknown, these results suggest the muscle-specific expression of Akmlc2f transcript and the functional activation of Akmlc2f promoter by C/EBPβ.

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Complete mitochondrial genome of the Korean bitterlingAcheilognathus koreensis(Cypriniformes; Cyprinidae)

Cited by 8 publications

References 8 publications

Characterization of the complete mitogenome of the endangered freshwater fish Gobiobotia naktongensis from the Geum River in South Korea: evidence of stream connection with the Paleo-Huanghe

Characterization of the complete mitogenome of the endangered freshwater fish Gobiobotia naktongensis from the Geum River in South Korea: evidence of stream connection with the Paleo-Huanghe

Description of Deefgea piscis sp. nov., and Deefgea tanakiae sp. nov., isolated from the gut of Korean indigenous fish

Molecular and Functional Analyses of the Fast Skeletal Myosin Light Chain2 Gene of the Korean Oily Bitterling, Acheilognathus koreensis

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