Complete mitochondrial genome of the two-spotted stag beetle,Metopodontus blanchardi(Coleoptera: Lucanidae)

Abstract

We have completely sequenced the mitochondrial genome (mitogeome) of the two-spotted stag beetle, Metopodontus blanchardi, which is listed as a first-degree endangered species in Korea. The complete mitogenome of M. blanchardi was determined to be 21,628 bp, indicating at least 5 kb larger in size than typical animal mitogenomes. Such a long M. blanchardi genome stems from a 3100-bp long A + T-rich region and a 4051-bp long, large non-coding sequence located between tRNA(Ile) and tRNA(Gln). The A + T-rich region is composed of duplicated repeat sequences (each 965 bp and 969 bp), and three non-repeat sequences encompassing the repeat sequences. The 4051-bp long non-coding sequence is composed of ∼ 17 tandem repeat sequences, each of which is composed of two subunits (113-bp and 104-bp long subunits) and this is encompassed by non-repeat sequences. The start codon for COI gene of M. blanchardi was designated as unconventional AAG (Lysine) by following a previous study.

Complete mitochondrial genome of a leaf-mining beetle, Rhadinosa nigrocyanea (Coleoptera: Chrysomelidae) with phylogenetic consideration

“…It contains 2 ribosomal RNA genes, 22 transfer RNAs, 13 protein-coding genes (PCGs) and a 3002 bp long non-coding A þ T-rich region. Its gene content and orientation are typical of other leaf beetle mitochondrial genomes and identical to those of the putative ancestor of insects (Kim et al 2015;Song et al 2017). …”

“…The total genomic DNA isolated from the head tissue was used to decipher its complete mitochondrial genome. The circular mitogenome of R. nigrocyanea was 17,965 bp, A þ T rich (76.6%), and includes 37 genes (GenBank accession MF041978) (Table 1), and this size is moderate compared to other Coleoptera species (Bae et al 2004;Kim et al 2015). It contains 2 ribosomal RNA genes, 22 transfer RNAs, 13 protein-coding genes (PCGs) and a 3002 bp long non-coding A þ T-rich region.…”

Description of Nearly Completed Mitochondrial Genome Sequences of the Garden Chafer Polyphylla laticollis manchurica, Endangered in Korea (Insecta: Coleoptera)

“…We believe this may have happened because this region is exceptionally long, containing unexpectedly long repeat regions and a high A/T content, considering previous sequence results of other coleopteran species, such as P. brevitarsis and M. blanchardi (Kim et al, 2013a(Kim et al, , 2013b. Primer information for each short fragment, long fragment, and internal primers for primer walking are provided (Table 1).…”

“…In particular, the complete mitogenome sequence of the infraorder Scarabaeiformia in Polyphaga is available only for two species (Cameron et al, 2009;Sheffield et al, 2009). Recently, Kim et al (2013aKim et al ( , 2013b additionally reported two complete mitogenome sequences of Scarabaeiformia, the whiter-spotted flower chafer, Protaetia brevitarsis (Scarabaeidae) and the two-spotted stag beetle, M. blanchardi (Lucanidae). In this paper, we report the mitogenome sequence of Polyphylla laticollis manchurica to describe the genome via comparison to those of pre-existing coleopteran insects in terms of whole genome organization, arrangement, and the major characteristics of individual genes.…”

“…3). Thus, Sheffield et al (2008) previously suggested that start codon for COI genes should be chosen to minimize which contains a 4,051-bp long, large non-coding sequence located between tRNA Ile and tRNA Gln , along with a 3,100-bp long A+T-rich region, resulting in a genome size of 21,628 bp, which is at least 5 kb larger than typical animal mitogenomes (Kim et al, 2013b). Considering these, it would be a reasonable speculation that the unfinished region is unusually large.…”