Complete mitochondrial genome of the honeybee Apis cerana native to two remote islands in Japan

Okuyama, Hisashi; Wakamiya, Takeshi; Fujiwara, Ayumi; Washitani, Izumi; Takahashi, Junichi

doi:10.1007/s12686-017-0721-5

Cited by 9 publications

(7 citation statements)

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“…1). Overall, it comprises 42.3% A, 41.8% T, 6.2% G, and 9.7% C, contains at most AT (84%) and at least CG (16%), which very similar to A. c. japonica (AP017941, AP017985, AP017314), A. c. cerana (AP017984, AP017983, KM244704, GQ162109) (Tan et al, 2011;Takahashi et al, 2016;Okuyama et al, 2017), A. m. ligustica (NC_001566) (Crozier & Crozier, 1993), A. m. caucasica (AP018404), A. m. carpathica (AP018403), A. m. meda (KY464957) and A. m. syriaca (GenBank -KY926882). Korean A. cerana mtDNA is characterized by the highest frequencies of AA, AT, TT and TA dinucleotides (>16% each) and lowest frequencies of GG, GC, CG dinucleotides (<1% each).…”

Section: Resultsmentioning

confidence: 99%

“…To explore the phylogeny of Korean A. cerana, additional A. cerana samples were taken from the DDBJ/Genbank database: GQ162109, KM244704 (A. c. cerana, China, Yunnan) (Tan et al, 2011), AP017983 (A. cerana, China, Jiangsu) (Okuyama et al, 2017), AP017314 (A. c. japonica, Japan, Kyoto) (Takahashi et al, 2016), AP017941 (A. c. japonica, Japan, Amami), AP017985 (A. c. japonica, Japan, Tsushima), AP017984 (A. cerana, Taiwan, Taipei) (Okuyama et al, 2017) (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

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Phylogenetic Uniqueness of Honeybee Apis Cerana from the Korean Peninsula Inferred from The Mitochondrial, Nuclear, and Morphological Data

Il’yasov

Park²,

Takahashi

et al. 2018

Journal of Apicultural Science

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Apis cerana is an Eastern honeybee species distributed throughout Asia and closely related to the Western honeybee species Apis mellifera distributed across all of Africa, Europe and Western Asia, and subdivided into thirty confirmed subspecies. Currently, A. cerana is an endangered bee species in contrast to A. mellifera. We sequenced and annotated the complete mitochondrial genome of A. cerana from the Jeollanam-do province of South Korea and uploaded to the DDBJ/Genbank database(AP018431). MtDNA sequence is 15.925 bp long, has 84% AT-content and 16% GC-content and contains 22 tRNA genes, 13 protein-coding genes, two ribosomal RNA genes, one AT-rich region and four non-coding intergenic regions (NC1-4). All protein-coding genes are started by ATT and ATG codons, except the genes ATP8 and ND4, which started by ATC and ATA, respectively, and are stopped by the common codons TAA and TAG. A comparative analysis of the whole mtDNA sequences of A. cerana from Korea and Taiwan, A. c. cerana from China and A. c. japonica from Japan showed that the genetic divergence of the Korean A. cerana sample from subspecies A. c. cerana (2.57%) and A. c. japonica (2.58%) matched to the level of genetic divergence of mtDNA between animal subspecies (0.8-8%). Based on the comparative analysis of complete mtDNA (~16,000 bp), two nuclear gene VG and EF1- α sequences (~8,000 bp) and morphological measurements (six parameters), we assumed that Korean A. cerana, Chinese A. c. cerana and Japanese A. c. japonica are different subspecies at an early stage of sub-speciation and could be called further as subspecies of Apis cerana koreana.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Phylogenetic Uniqueness of Honeybee Apis Cerana from the Korean Peninsula Inferred from The Mitochondrial, Nuclear, and Morphological Data

Il’yasov

Park²,

Takahashi

et al. 2018

Journal of Apicultural Science

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“…The ATP6 and ATP8 genes shared 19 nucleotides. Nine protein-coding genes of the A. cerena mitochondrial genome started with ATT; ATP6, COIII, and Cytb genes, with ATG; and ATP8 gene, with ATC, all of which have been commonly found in the A. cerana mitochondrial genome (Tan et al 2011;Takahashi et al 2016;Okuyama et al 2017). The stop codon of each of these protein-coding genes was either TAA, similar to the case in other honeybees.…”

mentioning

confidence: 87%

“… 2016 ; Okuyama et al. 2017 ). The stop codon of each of these protein-coding genes was either TAA, similar to the case in other honeybees.…”

mentioning

confidence: 99%

The complete mitochondrial genome of the cavity-nesting honeybee, Apis cerana (Insecta: Hymenoptera: Apidae) from Borneo

Okuyama

Tingek

Takahashi

2017

Mitochondrial DNA Part B

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The complete mitochondrial genome of the cavity-nesting honeybee Apis cerana from Sabah on Borneo Island was analyzed using next-generation sequencing. The mitochondrial genome of A. cerana was a circular molecule of 15,884 bp and was similar to that of the other cavity-nesting honeybee species. The average AT content in the A. cerana mitochondrial genome was 84.4%. It was predicted to contain 13 protein-coding, 22 tRNA, and two rRNA genes, along with one A þ T-rich control region.

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“…Table S2: Mitochondrial sequences of honeybees ( A. cerana , A. dorsata and A. mellifera ) used to design species-specific primers. Figure S1: Preliminary specificity test of ACF/ACR (Suarez et al, 2018) using DNA extracts of honeybees [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ].…”

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confidence: 99%

DNA-Based Method for Traceability and Authentication of Apis cerana and A. dorsata Honey (Hymenoptera: Apidae), Using the NADH dehydrogenase 2 Gene

Namin

Yeasmin

Choi

et al. 2022

Foods

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Honey is a widely used natural product and the price of honey from Apis cerana (ACH) and A. dorsata (ADH) is several times more expensive than the one from A. mellifera (AMH), thus there are increasing fraud issues reported in the market by mislabeling or mixing honeys with different entomological origins. In this study, three species-specific primers, targeting the NADH dehydrogenase 2 (ND2) region of honeybee mitochondrial DNA, were designed and tested to distinguish the entomological origin of ACH, ADH, and AMH. Molecular analysis showed that each primer set can specifically detect the ND2 region from the targeted honeybee DNA, but not from the others. The amplicon size for A. cerana, A. dorsata and A. mellifera were 224, 302, and 377 bp, respectively. Importantly, each primer set also specifically produced amplicons with expected size from the DNA prepared from honey samples with different entomological origins. The PCR adulteration test allowed detection of 1% of AMH in the mixture with either ACH or ADH. Furthermore, real-time PCR and melting curve analysis indicated the possible discrimination of origin of honey samples. Therefore, we provide the newly developed PCR-based method that can be used to determine the entomological origin of the three kinds of honey.

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Complete mitochondrial genome of the honeybee Apis cerana native to two remote islands in Japan

Cited by 9 publications

References 9 publications

Phylogenetic Uniqueness of Honeybee Apis Cerana from the Korean Peninsula Inferred from The Mitochondrial, Nuclear, and Morphological Data

Phylogenetic Uniqueness of Honeybee Apis Cerana from the Korean Peninsula Inferred from The Mitochondrial, Nuclear, and Morphological Data

The complete mitochondrial genome of the cavity-nesting honeybee, Apis cerana (Insecta: Hymenoptera: Apidae) from Borneo

DNA-Based Method for Traceability and Authentication of Apis cerana and A. dorsata Honey (Hymenoptera: Apidae), Using the NADH dehydrogenase 2 Gene

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