Complete mitochondrial genome of ocellated blenny, Opisthocentrus ocellatus (Tilesius, 1811) (Zoarcales: Opisthocentidae)

Abstract

The complete mitochondrial genome (mitogenome) of Opisthocentrus ocellatus (Zoarcales: Opisthocentridae) was sequenced for the first time. The whole genome was 16,525 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes (tRNA), two ribosome (rRNA) genes, and two non-coding regions. The overall base composition of the mitochondrial DNA (mtDNA) was 26.2% for A, 28.3% for T, 27.4% for C, and 18.1% for G. Six protein-coding genes include incomplete stop codons while others have standard ones, and start codons are typically ATG, GTG, ATA, or ATC. The phylogenetic analysis, which based on the mitogenomes of O. ocellatus and other 14 fish species from Zoarcales infraorder indicated that O. ocellatus is more closely related to the family Pholidae than to Stichaeidae.

Complete mitochondrial genome of Stichaeus nozawae Jordan & Snyder 1902 (Zoarcales: Stichaeidae)

“…Genome annotation revealed 13 coding fragments with most of the genes residing on the heavy strand while ND6 as well as eight tRNAs were encoded on the light-strand. It contained 22 tRNAs along with additional tRNA-Leu and tRNA-Ser, both having atypical codons as it was also found in many eelpouts (see Ayala et al 2017;Rutenko et al 2019). The annotation also revealed 16S and 12S rRNAs and CR, 862 pb in length.…”

Phylogenetic Classification of Living and Fossil Ray-Finned Fishes (Actinopterygii)

“…Morphological (Clardy 2014) and molecular (Radchenko et al 2010;Turanov et al 2012;Chereshnev et al 2013;Radchenko 2015Radchenko , 2016Radchenko , 2017Betancur-R et al 2017;Rutenko et al 2019;Hotaling et al 2021) phylogenetic analyses resolve the traditional delimitation of Stichaeidae (pricklebacks) (Makushok 1958;Mecklenburg and Sheiko 2004;Zemnukhov 2012;J. S. Nelson et al 2016:480) as nonmonophyletic.…”

“…Morphological studies resolve a clade containing Scytalina and Xiphister or place Scytalina as the sister lineage of a clade containing Phytichthys, Xiphister, Ptilichthys goodei (Quillfish), and Pholidae (gunnels) (Hilton 2009;Clardy 2014). At least five other lineages traditionally classified as Stichaeidae are more closely related to other lineages of Zoarcoidea in molecular and morphological phylogenetic analyses: Opisthocentridae (rearspined pricklebacks) is the sister lineage of a clade containing Ptilichthys and Pholidae (Figure 18; Radchenko et al 2012;Chereshnev et al 2013;Kwun 2013;Kwun and Kim 2013;Radchenko 2015Radchenko , 2016Rutenko et al 2019;Hotaling et al 2021;Ghezelayagh et al 2022); Lumpenidae (eel pricklebacks) is the sister lineage of Cryptacanthodes (wrymouths) (Figure 18; Kwun and Kim 2013;Radchenko 2015Radchenko , 2016Hotaling et al 2021;Ghezelayagh et al 2022); Cebidichthyidae (monkeyfaces), including Cebidichthys, Dictyosoma, Esselenia, and Esselenichthys, was formerly classified in the stichaeid subclade Xiphisterinae but is now resolved as the sister lineage of all other Zoarcoidea to the exclusion of Bathymasteridae and possibly Eulophiidae (Figure 18; Radchenko et al 2012;Turanov et al 2012;Chereshnev et al 2013;Radchenko 2015Radchenko , 2016Hotaling et al 2021;Ghezelayagh et al 2022); Neozoarcidae (kissing eelpouts) is resolved as sister to a clade containing Anarhichadidae (wolffishes) and Zoarcidae (eelpouts) (Figure 18; Radchenko 2015Radchenko , 2016Turanov et al 2017;…”