Complete mitochondrial DNA sequences of Saccostrea mordax and Saccostrea cucullata : genome organization and phylogeny analysis

Abstract

Classified in the phylum mollusks, oysters are bivalves which are found in estuaries and coastal zones. Because of their plastic shell, mitochondrial DNA analysis of this species becomes an interesting field, necessary to investigate their phylogenetic and evolution of relations. In our study, two oyster species: Saccostrea mordax and Saccostrea cucullata from Indian Ocean (Madagascar) were investigated. The complete sequence of Saccostrea mordax (16 512 bp) and Saccostrea cucullata (16 396 bp) were described and determined, with their mitogenomes deposited in the GenBank with accession number KP769562 and KP967577 respectively. Both mitochondrial genome sequences contained 12 protein-coding genes, 23 tRNAs, and two rRNAs, all encoded in the same heavy strand. High levels of similarity in the gene arrangement of the two Saccostrea species were evident. The phylogenetic analysis shows a closer relationship between the two Saccostrea species and confirms the strong relationship within Saccostrea, Crassostrea and Ostrea genus in taxonomy of Ostreidae family.

The Complete Mitochondrial Genome Sequences of the Philomycus bilineatus (Stylommatophora: Philomycidae) and Phylogenetic Analysis

“…In contrast, the GC skew values show a large difference, and the most significant difference is seen in the two genes nad4L and nad4 , which are negative for P. bilineatus and positive for M. bilineatum , respectively. This result suggests that more Ts and Gs are present in most PCGs, which is consistent with most previous observations [41].…”

The complete mitochondrial genome of Saccostrea malabonensis (Ostreida: Ostreidae): characterization and phylogenetic position

“…Our phylogenetic analysis supports the current established taxonomic framework among the three genera of Crassostrea , Ostrea and Saccostrea in Ostreidae, and the relationships have also been verified by other molecular phylogenetic studies (Volatiana et al. 2016 ; Wu JH et al. 2019 ; Li et al.…”

“…Saccostrea mytiloides NC_036479, Saccostrea glomerata NC_036483, Saccostrea echinata NC_036478, Saccostrea kegaki NC_030533, Saccostrea cucullata NC_027724 (Volatiana et al. 2016 ), Saccostrea malabonensis ON649706 (This study), Saccostrea mordax NC_013998, Ostrea lurida NC_022688 (Xiao et al. 2015 ), Ostrea denselamellosa NC_015231 (Yu and Li 2011 ), Crassostrea virginica NC_007175, Crassostrea iredalei NC_013997 (Wu X et al.…”

The complete mitochondrial genome of the tropical oyster Saccostrea echinata (Bivalvia: Ostreidae) from the South China Sea

“…Species in genus Saccostrea are well known for the highly morphological plasticity with different habitat conditions that morphological diagnosis are extremely difficult and time-consuming (Lam and Morton 2006;Amaral and Luiz 2016). DNA-sequenced based methods provide an opportunity to construct additional taxon diagnosis systems that employ DNA sequences as 'barcodes' to identify oyster and to conduct further phylogenetic studies (Sekino and Yamashita 2016;Volatiana et al 2016;Cui 2018;Samantha et al 2019). Here, we sequenced and annotated mitogenome of S. echinata which will provide essential molecular information for the genetical understanding of this oyster and thus contribute to better management and sustainable use of the species.…”

“…DNA-sequenced based methods provide an opportunity to construct additional taxon diagnosis systems that employ DNA sequences as ‘barcodes’ to identify oyster and to conduct further phylogenetic studies (Sekino and Yamashita 2016 ; Volatiana et al. 2016 ; Cui 2018 ; Samantha et al. 2019 ).…”