“…Mutations in genes controlling NAD cis/trans-processing have been described in N. sylvestris, maize, and Arabidopsis (Arabidopsis thaliana; for review, see Colas des Francs-Small and Small, 2014), and insertion mutants lacking nucleus-encoded subunits have been characterized in Arabidopsis (Meyer et al, 2009;Kühn et al, 2015;Soto et al, 2015). Impaired holocomplex assembly/activity are common features of all plant mutants characterized so far, but putative intermediate assembly forms have been observed in several cases (Gutierres et al, 1997;Karpova and Newton, 1999;Perales et al, 2005;Pineau et al, 2008;Meyer et al, 2009Meyer et al, , 2011Kühn et al, 2015). A general respiratory impairment, measured as either oxygen consumption of leaf discs or CO 2 emission by attached leaves in the dark (dark respiration), has not been observed, and in organello experiments showed the induction of nonphosphorylating NAD(P)H dehydrogenases (Rasmusson et al, 2008) in all cases analyzed (Marienfeld and Newton, 1994;Sabar et al, 2000;Meyer et al, 2009;Keren et al, 2012).…”