Complete mitochondrail genome of Corydoras agassizii

Abstract

We reported the complete mitochondrial genome yielded using next-generation sequencing of Corydoras agassizii in this study. The total length of the mitochondrial genome is 16,562 bp, with the base composition of 32.6% A, 25.9% T, 26.8% C, and 14.7% G, in several. It contains two rRNA genes, 22 tRNA genes, 13 protein-coding genes, and a 945 bp non-coding control region (D-loop region). The sequence of these genes is consistent with that found in the Siluriformes. The complete mitogenomes of C. agassizii and other 17 species of fish were constructed by phylogenetic analysis using Neighbour-Joining method. The topological structure indicated that species participating in the analysis belong to three groups (Siluridae, Loricariidae, and Callichthyidae) of nine genera, and the C. agassizii was clustered with other species from genus Corydoras. The external morphological characteristics of C. agassizii are consistent with the results of molecular classification, so the mitogenome can be used to identify Corydoras species in the future. ARTICLE HISTORY

Comparative analysis of the mitogenomes of two Corydoras (Siluriformes, Loricarioidei) with nine known Corydoras, and a phylogenetic analysis of Loricarioidei

“…The two mitogenomes were circular and contained 37 mitochondrial genes (13 PCGs, 22 tRNA genes, and two rRNA genes) and one D-loop. The position of each gene in the mitogenome was identical to that in other species of Corydoras (Saitoh et al 2003;Moreira et al 2016aMoreira et al , 2017Liu et al 2019a, b, c, d;Chen et al 2020;Lv et al 2020). One of the 13 PCGs (ND6) and eight tRNAs (tRNA-Ala, tRNA-Cys, tRNA-Glu, tRNA-Asn, tRNA-Pro, tRNA-Gln, tRNA-Ser(TGA), and tRNA-Tyr) were encoded by the light chain (-), whereas the other 28 genes, including 12 PCGs, 14 tRNAs, two rRNAs, and one D-loop, were encoded by the heavy chain (+) (Fig.…”

“…material 1: Figs S2, S3. The PCGs of the eleven mitogenomes of Corydoras (Saitoh et al 2003;Moreira et al 2016aMoreira et al , 2017Liu et al 2019a, b, c, d;Chen et al 2020;Lv et al 2020) translate into 3,798-3,802 codons and showed very similar codon usage, excluding the stop codons (26-28 bp). Ile (310.82 ± 2.69 codons), Thr (312.64 ± 2.27 codons), Ala (312.73 ± 3.08 codons), and Leu1 (CUN) (475.45 ± 12.89 codons) were the four most predominant codon families and may be associated with the coding function of the chondriosome.…”

“…In this study, the complete mitogenomes of two species of Corydoras (Bronze corydoras C. aeneus Gill, 1858 and peppered corydoras C. paleatus Jenyns, 1842) were sequenced, assembled, and annotated. The genome organization, gene contents, repeat sequences, and tRNA structures of the eleven mitogenomes were compared and analyzed in combination with nine published mitogenomes of Corydoras (Saitoh et al 2003;Moreira et al 2016aMoreira et al , 2017Liu et al 2019a, b, c, d;Chen et al 2020;Lv et al 2020). Determining the similarities and differences in gene orders, genetic structures, base compositions, evolutionary features, and codon usage can provide molecular insights into the taxonomic and phylogenetic characteristics of the order Siluriformes.…”