Complete In Vitro Prosthesis Endothelialization Induced by Artificial Extracellular Matrix

Pollara, P.; Alessandri, Giulio; Bonardelli, Stefano; Simonini, Americo; Cabibbo, Enzo; Portolani, Nazario; Ga, Tiberio; Sm, Giulini; Turano, A

doi:10.1080/089419399272638

Cited by 12 publications

(1 citation statement)

References 13 publications

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“…The incorporation of single ECM components may present advantages over utilization of the complete matrix alone due to their selective well-defined biological activities and function [156–160]. However, single components of matrix or peptides derived from one type of ECM protein may have a lower activity compared to the native ECM ligand due to the absence of complementary or modulatory domains and amino acids.…”

Section: Vascular Tissue Developmentmentioning

confidence: 99%

ECM-Based Materials in Cardiovascular Applications: Inherent Healing Potential and Augmentation of Native Regenerative Processes

Piterina

Cloonan

Meaney

et al. 2009

IJMS

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The in vivo healing process of vascular grafts involves the interaction of many contributing factors. The ability of vascular grafts to provide an environment which allows successful accomplishment of this process is extremely difficult. Poor endothelisation, inflammation, infection, occlusion, thrombosis, hyperplasia and pseudoaneurysms are common issues with synthetic grafts in vivo. Advanced materials composed of decellularised extracellular matrices (ECM) have been shown to promote the healing process via modulation of the host immune response, resistance to bacterial infections, allowing re-innervation and reestablishing homeostasis in the healing region. The physiological balance within the newly developed vascular tissue is maintained via the recreation of correct biorheology and mechanotransduction factors including host immune response, infection control, homing and the attraction of progenitor cells and infiltration by host tissue. Here, we review the progress in this tissue engineering approach, the enhancement potential of ECM materials and future prospects to reach the clinical environment.

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Section: Vascular Tissue Developmentmentioning

confidence: 99%

ECM-Based Materials in Cardiovascular Applications: Inherent Healing Potential and Augmentation of Native Regenerative Processes

Piterina

Cloonan

Meaney

et al. 2009

IJMS

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Enhanced retroviral transduction of 293 cells cultured on liquid-liquid interfaces

Kwon

Peng

2000

Biotechnol. Bioeng.

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In clinical research, retrovirus‐mediated gene therapy is one of the most commonly used methods to deliver and express the gene of interest due its ability to allow for stable gene integration into the chromosomes of target cells. To elevate the efficiency of viral transduction, several restrictions, such as low virus–cell encounters and the necessity for cell division, must be improved. In this study, we focused on the possibility of accelerating cell division and the ensuing increment of viral transduction on flexible substrata. Perfluorocarbon FC‐40 was harnessed to form a liquid–liquid interface with culture medium. Enhanced green fluorescence protein (EGFP) was employed as the marker gene to quickly illustrate the percentage of viral infection. The results indicate that the gene transfer efficiency to 293 cells cultured on protein‐precoated liquid–liquid interfaces was higher than in cells cultured on rigid polystyrene surfaces. This increased transduction rate on the liquid–liquid interface is consistent with the acceleration of division of 293 cells on a flexible interface, which exhibited less adhesiveness. The effect of cell–cell contact inhibition on the rate of gene transduction is also addressed in this study. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 331–338, 2001

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Fibronectin and culture temperature modulate the efficacy of an avidin–biotin binding system for chondrocyte adhesion and growth on biodegradable polymers

Tsai

Wang

Chang

et al. 2007

Biotech & Bioengineering

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Cell adhesion to a scaffold is a prerequisite for tissue engineering. Many studies have been focused on enhancing cell adhesion to synthetic materials that are used for scaffold fabrication. Previously, we showed that immobilization of biotin molecules to chondrocyte surfaces enhanced cell adhesion to avidin-coated biodegradable polymers such as poly-L-lactic acid, poly-D,L-lactic acid and polycaprolactone. However, the endocytosis of cell membrane biotin molecules decreases binding strength between biotinylated-chondrocytes (B-chondrocytes) and avidin-coated substrata, and therefore decreases cell spreading and discourages long-term chondrocytes culture. In this study, we proposed two strategies to solve the shortcoming of the avidin-biotin binding system. First, the avidin-biotin binding system is combined with the intrinsic integrin-dependent adhesion systems in order to enhance long-term cell culture. Second, the incubation temperature is lowered in order to slow down the endocytosis process. We found that the avidin-biotin binding system in combination with FN-integrin binding system enhanced cell adhesion, cell spreading and cell growth. Decrease of cell culture temperature to 4 degrees C enhanced the adhesion of B-chondrocytes to the avidin-coated surfaces, but decreased cell viability and proliferation, compared to culture temperature of 37 degrees C. Whether there is an optimal seeding temperature between 4 and 37 degrees C for both adhesion and proliferation of B-chondrocytes needs further investigation. Our results indicated that modulation of the adhesion conditions could further enhance the efficacy of the avidin-biotin binding system in mediating cell adhesion, and subsequent tissue culture.

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Complete In Vitro Prosthesis Endothelialization Induced by Artificial Extracellular Matrix

Cited by 12 publications

References 13 publications

ECM-Based Materials in Cardiovascular Applications: Inherent Healing Potential and Augmentation of Native Regenerative Processes

ECM-Based Materials in Cardiovascular Applications: Inherent Healing Potential and Augmentation of Native Regenerative Processes

Enhanced retroviral transduction of 293 cells cultured on liquid-liquid interfaces

Fibronectin and culture temperature modulate the efficacy of an avidin–biotin binding system for chondrocyte adhesion and growth on biodegradable polymers

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