Complete Horizontal Skin Cell Resurfacing and Delayed Vertical Cell Infiltration into Porcine Reconstructive Tissue Matrix Compared to Bovine Collagen Matrix and Human Dermis

Mirastschijski, Ursula; Kerzel, Corinna; Schnabel, Reinhild; Strauß, Sarah; Breuing, Karl-Heinz

doi:10.1097/prs.0b013e31829fe461

Cited by 13 publications

(10 citation statements)

References 36 publications

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“…The decellularized dermis utilized here, Strattice, has been used successfully as an internally placed scaffold for treatment of subcostal hernia repair [ 54 ] and breast reconstruction [ 55 , 56 ]. Mirastschijski et al have found that the decellularized dermis may be best suited for dermal wound beds that require a higher mechanical load than in those previously mentioned [ 57 ]. While residual porosity does facilitate some AdMSC migration, the high mortality rate would make this an unsuitable scaffold for a cell seeded dermal wound treatment.…”

Section: Discussionmentioning

confidence: 99%

In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Wahl

Fierro

Peavy

et al. 2015

BioMed Research International

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Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.

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Section: Discussionmentioning

confidence: 99%

In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Wahl

Fierro

Peavy

et al. 2015

BioMed Research International

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“…It has been established that epithelial resurfacing and vertical proliferation were reduced and delayed by application of dermal matrices without affecting apoptosis, cell differentiation, or basement membrane formation . Previous clinical reports have also shown the feasibility of simultaneously applying thin split‐thickness skin grafts over a collagen–elastin matrix for full‐thickness skin reconstruction in burns or cancer reconstruction but lacked an explanation on how the applied skin grafts received nutrients, in other words: how the “mechanism of take” of the skin graft occurred.…”

Section: Discussionmentioning

confidence: 99%

“…However, the subjective assessment of scar quality by use of the Vancouver Scar Scale was not validated for pigs, and an objective assessment of dermal thickness was lacking . We aimed at providing a scientific basis for the explanation of graft supply in a simultaneous application of a dermal matrix and split‐thickness skin graft …”

Section: Discussionmentioning

confidence: 99%

Simultaneous dermal matrix and autologous split‐thickness skin graft transplantation in a porcine wound model: A three‐dimensional histological analysis of revascularization

Wiedner

Tinhofer

Kamolz

et al. 2014

Wound Repair Regeneration

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Despite the popularity of a simultaneous application of dermal matrices and split-thickness skin grafts, scarce evidence exists about the process of revascularization involved. In this study, we aimed at analyzing the progression of revascularization by high-resolution episcopic microscopy (HREM) in a porcine excisional wound model. Following the surgical procedure creating 5 × 5 cm(2) full-thickness defects on the back, one area was covered with an autologous split-thickness skin graft alone (control group), the other with a collagen-elastin dermal matrix plus split-thickness skin graft (dermal matrix group). Two skin biopsies per each group and location were performed on day 5, 10, 15, and 28 postoperatively and separately processed for H&E as well as HREM. The dermal layer was thicker in the dermal matrix group vs. control on day 5 and 28. No differences were found for revascularization by conventional histology. In HREM, the dermal matrix did not appear to decelerate the revascularization process. The presence of the dermal matrix could be distinguished until day 15. By day 28, the structure of the dermal matrix could no longer be delineated and was replaced by autologous tissue. As assessed by conventional histology and confirmed by HREM, the revascularization process was comparable in both groups, notably with regard to the vertical ingrowth of sprouting vessels. The presented technique of HREM is a valuable addition for analyzing small vessel sprouting in dermal matrices in the future.

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“…H-KC and hd-FB isolation and the cell culture experiments were described in our previous work [57]. In short, the procedure involves a peeling of the epidermis by dispase II (2 U/mL; Roche, Grenzach-Wyhlen, Germany) digestion and a digestion with 0.05% trypsin-ethylenediaminetetraacetic acid solution (T/E) (PAA; L11-003) for h-KC.…”

Section: Cell Isolation and Culturementioning

confidence: 99%

Water-based, surfactant-free cytocompatible nanoparticle-microgel-composite biomaterials – rational design by laser synthesis, processing into fiber pads and impact on cell proliferation

et al. 2017

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Abstract:This work highlights the laser-based aqueous synthesis and processing of nanocomposites, composed of zinc or iron nanoparticles embedded in a N-Vinylcaprolactam microgel matrix, with potential applicability as ion releasing fiber pads for wound healing. An in situ laser process for microgel synthesis is developed and optimized for high embedded nanoparticle yields, evaluating influences of laser repetition rate and monomer concentration. The impact of the nanoparticles on polymerization was increased by embedded zinc oxide nanoparticles, and reduced in the presence of iron oxide. Furthermore, TEM images verified that the nanoparticles were homogeneously embedded into the polymer matrix. The nanoparticle-loaded microgels were thermally stable up to 429°C, which ensures that the composites maintain their integrity after heat sterilization and during rapid prototyping by thermal polymer processing. The general suitability of the hydrogels as active biomaterial for wound healing was assessed in toxicity, cell proliferation and migration assays using human dermal fibroblasts and keratinocytes, where cytocompatibility was verified, while the proliferation was affected by the gel alone as well as the embedded nanoparticles. The hydrogels were processed to suit their use as a biomaterial for wound coverages via electrospinning resulting in a centimeter scale fully cytocompatible fiber pad with the nanoparticle-filled microgel capsules supported on the fiber's surface.

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Complete Horizontal Skin Cell Resurfacing and Delayed Vertical Cell Infiltration into Porcine Reconstructive Tissue Matrix Compared to Bovine Collagen Matrix and Human Dermis

Cited by 13 publications

References 36 publications

In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

Simultaneous dermal matrix and autologous split‐thickness skin graft transplantation in a porcine wound model: A three‐dimensional histological analysis of revascularization

Water-based, surfactant-free cytocompatible nanoparticle-microgel-composite biomaterials – rational design by laser synthesis, processing into fiber pads and impact on cell proliferation

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