Complete ¹H and 13C NMR assignments and anti fungal activity of two 8-hydroxy flavonoids in mixture

Johann, Susana; Smânia-Jr, Artur; Pizzolatti, Moacir Geraldo; Schripsema, Jan; Braz‐Filho, Raimundo; Branco, Alexsandro

doi:10.1590/s0001-37652007000200004

Cited by 14 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to these mass spectra data it may be 5-hydroxy-3,6,7,3',4'-pentamethoxy fl avone as shown in Figure 6. [28,29] It was supported and confi rmed by bands observed in IR spectra and signals recorded in H' NMR spectra.…”

Section: Discussionmentioning

confidence: 76%

Antieosinophilic activity of various subfractions of leaves of Vitex negundo

Patel

Deshpande²

2013

Int J Nutr Pharmacol Neurol Dis

View full text Add to dashboard Cite

Aim:The present study was undertaken to isolate and identify active constituent of leaves of Vitex negundo for their actions on bronchial hyperresponsiveness. Materials and Methods: Effects of aqueous subfraction, acetone subfraction, and chloroform sub-fraction on bronchial hyperresponsiveness and serum bicarbonate level were studied using egg-albumin-induced asthma in guinea pigs. The structure of aqueous subfraction of leaves of Vitex negundo was determined by various spectroscopy methods like UV spectroscopy, FT-IR spectroscopy, EI-mass spectroscopy, and H 1 NMR spectroscopy. Results: Animals pretreated with aqueous subfraction of leaves of Vitex negundo showed signifi cantly lesser serum bicarbonate level, when compared with untreated sensitized animals. Animals pretreated with aqueous subfraction showed signifi cant lesser eosinophils count (9.50 ± 1.5044; P < 0.05) when compared with untreated sensitized animals (29.50 ± 2.5074; P < 0.05). Histopathology of lungs in sensitized guinea pig showed infi ltration of infl ammatory cells like eosinophils and neutrophils which is due to marked infl ammation in lungs. Histopathology of lungs in animals pretreated with aqueous subfraction (200 mg/ kg) showed normal airway, blood vessels, and bronchoalveolar space. Conclusion: It can be concluded that aqueous subfraction of leaves of Vitex negundo possesses antieosinophilic activity. It reduces bronchial hyperresponsiveness. As per UV, EI-Mass, FT-IR, and H 1 NMR spectra analysis, the structure of aqueous subfraction is determined as a 5-hydroxy-3,6,7,3',4'-pentamethoxy fl avone. It may be useful in the treatment of asthma and various infl ammatory, allergic, and immunologic diseases.

show abstract

Section: Discussionmentioning

confidence: 76%

Antieosinophilic activity of various subfractions of leaves of Vitex negundo

Patel

Deshpande²

2013

Int J Nutr Pharmacol Neurol Dis

View full text Add to dashboard Cite

show abstract

“…Lime juice and its essential oil are also commonly used in the food, drug, and cosmetic industries because of their medicinal properties and fragrance. The traditional and pharmacological uses of Citrus aurantifolia L. plants are attributed to the presence of secondary plant metabolites including flavonoids, coumarins, and terpenoids 5, 6, 7…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman

Al-Aamri

Al-Abousi

Al-Jabri

et al. 2018

Journal of Taibah University Medical Sciences

View full text Add to dashboard Cite

Objectives This study investigated the chemical composition and the antioxidant and antimicrobial activities of the essential oil isolated from the aerial parts of Citrus aurantifolia L. Methods Fresh Citrus aurantifolia L. leaves were collected from farms in Sur city, located in the Al-Sharqia (Eastern) region of the Sultanate of Oman, during June–July of 2015. The essential oil was isolated using hydrodistillation. Gas chromatography–mass spectrometry (GC–MS) was used to identify and quantify the chemical constituents of the oil. An in-vitro 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method was used to determine the antioxidant activity of the isolated oil from the lime leaves while a disc diffusion method was used to evaluate the antibacterial activity against Gram-negative and Gram-positive bacteria. Results Thirty-three chemical compounds were identified, with d -limonene (63.35%) forming the major constituent. Other prominent constituents include 3,7-dimethyl-2,6-octadien-1-ol (7.07%), geraniol (6.23%), E -citral (4.35%), Z -citral (3.29%), and β-ocimene (2.25%). The essential oil of Citrus aurantifolia L. leaves showed excellent antibacterial activity against Staphylococcus aureus and moderate activity against pathogenic Escherichia coli strains. The oil exhibited promising in-vitro antioxidant activity (IC 50 value = 21.57 μg/mL) but showed moderate antibacterial activities. Conclusions The essential oil from Omani lime leaves is characterized by a high d -limonene content, making it useful for food, pharmaceutical, and perfumery industries.

show abstract

“…Additionally, C. aurantifolia is used in traditional medicine as an antiseptic, anthelmintic, mosquito bite repellent, for stomach ailments, tonic, antiscorbutic, astringent, diuretic, headache, arthritis, digestive and appetite stimulant, and for colds, coughs and sore throats [ 13 , 14 ]. Previous investigations of C. aurantifolia have reported flavonoids, coumarins, and terpenoids [ 15 , 16 , 17 , 18 ]. Peel oil of C. auranifolia has been analyzed by GC-MS analysis several times [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Hexane Extract of Citrus aurantifolia and Anti-Mycobacterium tuberculosis Activity of Some of Its Constituents

et al. 2012

View full text Add to dashboard Cite

The main aim of this study was to isolate and characterize the active compounds from the hexane extract of the fruit peels of Citrus aurantiifolia, which showed activity against one sensitive and three monoresistant (isoniazid, streptomycin or ethambutol) strains of Mycobacterium tuberculosis H37Rv. The active extract was fractionated by column chromatography, yielding the following major compounds: 5-geranyloxypsoralen (1); 5-geranyloxy-7-methoxycoumarin (2); 5,7-dimethoxycoumarin (3); 5-methoxypsoralen (4); and 5,8-dimethoxypsoralen (5). The structures of these compounds were elucidated by 1D and 2D NMR spectroscopy. In addition, GC-MS analysis of the hexane extract allowed the identification of 44 volatile compounds, being 5,7-dimethoxycoumarin (15.79%), 3-methyl-1,2-cyclopentanedione (8.27%), 1-methoxy-ciclohexene (8.0%), corylone (6.93%), palmitic acid (6.89%), 5,8-dimethoxypsoralen (6.08%), α-terpineol (5.97%), and umbelliferone (4.36%), the major constituents. Four isolated coumarins and 16 commercial compounds identified by GC-MS were tested against M. tuberculosis H37Rv and three multidrug-resistant M. tuberculosis strains using the Microplate Alamar Blue Assay. The constituents that showed activity against all strains were 5 (MICs = 25–50 μg/mL), 1 (MICs = 50–100 μg/mL), palmitic acid (MICs = 25–50 μg/mL), linoleic acid (MICs = 50–100 μg/mL), oleic acid (MICs = 100 μg/mL), 4-hexen-3-one (MICs = 50–100 μg/mL), and citral (MICs = 50–100 μg/mL). Compound 5 and palmitic acid were the most active ones. The antimycobacterial activity of the hexane extract of C. aurantifolia could be attributed to these compounds.

show abstract

Complete ¹H and 13C NMR assignments and anti fungal activity of two 8-hydroxy flavonoids in mixture

Cited by 14 publications

References 22 publications

Antieosinophilic activity of various subfractions of leaves of Vitex negundo

Antieosinophilic activity of various subfractions of leaves of Vitex negundo

Chemical composition and in-vitro antioxidant and antimicrobial activity of the essential oil of Citrus aurantifolia L. leaves grown in Eastern Oman

Chemical Composition of Hexane Extract of Citrus aurantifolia and Anti-Mycobacterium tuberculosis Activity of Some of Its Constituents

Contact Info

Product

Resources

About