Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes

Padilla, Susana; Prieto, Karla; Dohm, David J.; Turell, Michael J.; Klein, Terry A.; Fernández, Roberto; Watts, Douglas M.; Lowen, Robert G.; Palacios, Gustavo; Pitt, M. Louise M.; Wiley, Michael R.; Nasar, Farooq

doi:10.1007/s00705-020-04647-x

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…(Mel.) pedroi Sirivanakarn & Belkin are natural enzootic vectors of VEEV in Columbia, Peru and Panama [30][31][32]. Culex (Mel.)…”

Section: Of 18mentioning

confidence: 99%

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Costa¹,

Guidez²,

Briolant³

et al. 2023

Preprint

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French Guiana mosquito fauna diversity encompasses 242 species, of which nearly half of them belong to Culex (Diptera: Culicidae) genus. Whereas several species from Culex genus are important vectors of arbovirus, scarce studies focus on Culex genus, notably due to the inaccurate morphological identification of field-caught females gender. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. Culex females from French Guiana field were dissected based on morphological classification. Abdomens were used for molecular identification using COI (cytochrome oxydase 1) gene. Legs and thorax of 169 specimens belonging to 13 Culex species,( i.e. Cx. declarator, Cx. nigripalpus, Cx. quinquefasciatus, Cx. usquatus, Cx. adamesi, Cx. dunni, Cx. eastor, Cx. idottus, Cx. pedroi, Cx. phlogistus, Cx. portesi, Cx. rabanicolus and Cx. spissipes) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were noticed. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical Culex species and will permit to enhance knowledge on this highly diverse Culex mosquitoes genus.

show abstract

“…(Mel.) pedroi Sirivanakarn & Belkin are natural enzootic vectors of VEEV in Columbia, Peru and Panama [30][31][32]. Culex (Mel.)…”

Section: Of 18mentioning

confidence: 99%

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Costa¹,

Guidez²,

Briolant³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…( Mel. ) pedroi (Sirivanakarn & Belkin, 1980) are natural enzootic vectors of VEEV in Columbia, Peru and Panama [ 30 , 31 , 32 ]. Culex ( Mel .)…”

Section: Introductionmentioning

confidence: 99%

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

et al. 2023

View full text Add to dashboard Cite

The mosquito (Diptera: Culicidae) fauna of French Guiana encompasses 242 species, of which nearly half of them belong to the genus Culex. Whereas several species of Culex are important vectors of arboviruses, only a limited number of studies focus on them due to the difficulties to morphologically identify field-caught females. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. Culex females collected in French Guiana were morphologically identified and dissected. Abdomens were used for molecular identification using the COI (cytochrome oxidase 1) gene. Legs and thorax of 169 specimens belonging to 13 Culex species, (i.e., Cx. declarator, Cx. nigripalpus, Cx. quinquefasciatus, Cx. usquatus, Cx. adamesi, Cx. dunni, Cx. eastor, Cx. idottus, Cx. pedroi, Cx. phlogistus, Cx. portesi, Cx. rabanicolus and Cx. spissipes) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were obtained. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical Culex species and will permit the enhancement of knowledge on this highly diverse genus.

show abstract

Complete genomic sequences of Venezuelan equine encephalitis virus subtype IIID isolates from mosquitoes

Cited by 2 publications

References 11 publications

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling

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