Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen
            <i>Methanothermobacter</i>
            sp. Strain KEPCO-1

Jeon, Byoung Seung; Kim, Hyun‐Jin; Go, Young Wook; Kim, Young Gook; Joo, Ji Sun; Choi, Okkyoung; Sang, Byoung In

doi:10.1128/mra.01034-19

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Wind and solar energy, as renewables, pose challenges for energy storage due to their intermittent nature. Power–to–Gas technology offers efficient storage solutions, utilizing hydrogenotrophic methanogens to convert H 2 and CO 2 into CH 4 suitable for grid injection and an ecofriendly option for energy storage ( 1 , 2 ).…”

Section: Announcementmentioning

confidence: 99%

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Prabhaharan,

Nair,

Park

et al. 2024

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Prabhaharan,

Nair,

Park

et al. 2024

Microbiol Resour Announc

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“…The agar, including a single colony, was then diced into 0.125-cm 3 pieces and sequentially subcultured. Identification by amplification and sequencing of the 16S rRNA gene was conducted using the primers from a previous study ( 5 ). THM-1 was classified as a member of the genus Methanothermobacter by phylogenetic comparison of the 16S rRNA genes using MEGA7 ( 6 , 7 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Methanothermobacter sp. Strain THM-1, a Thermophilic and Hydrogenotrophic Methanogen Isolated from an Anaerobic Reactor in South Korea

Jeon

Kim

et al. 2021

Microbiol Resour Announc

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Methanothermobacter sp. strain THM-1, a thermophilic and hydrogenotrophic methanogen, was isolated from an anaerobic reactor enriched with thermophilic methanogens. The genome of THM-1 shares 98.81% of its sequence with Methanothermobacter wolfeii isolate SIV6 and consists of 1,724,502 bp with 1,665 protein-coding genes, 50 noncoding RNAs, and a GC content of 48.6%.

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Phenotypic and genomic characterization of Methanothermobacter wolfeii strain BSEL, a CO ₂ -capturing archaeon with minimal nutrient requirements

Ale Enriquez,

Ahring

2024

Appl Environ Microbiol

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A new variant of Methanothermobacter wolfeii was isolated from an anaerobic digester using enrichment cultivation in anaerobic conditions. The new isolate was taxonomically identified via 16S rRNA gene sequencing and tagged as M. wolfeii BSEL. The whole genome of the new variant was sequenced and de novo assembled. Genomic variations between the BSEL strain and the type strain were discovered, suggesting evolutionary adaptations of the BSEL strain that conferred advantages while growing under a low concentration of nutrients. M. wolfeii BSEL displayed the highest specific growth rate ever reported for the wolfeii species (0.27 ± 0.03 h −1 ) using carbon dioxide (CO 2 ) as unique carbon source and hydrogen (H 2 ) as electron donor. M. wolfeii BSEL grew at this rate in an environment with ammonium (NH 4 + ) as sole nitrogen source. The minerals content required to cultivate the BSEL strain was relatively low and resembled the ionic background of tap water without mineral supplements. Optimum growth rate for the new isolate was observed at 64°C and pH 8.3. In this work, it was shown that wastewater from a wastewater treatment facility can be used as a low-cost alternative medium to cultivate M. wolfeii BSEL. Continuous gas fermentation fed with a synthetic biogas mimic along with H 2 in a bubble column bioreactor using M. wolfeii BSEL as biocatalyst resulted in a CO 2 conversion efficiency of 97% and a final methane (CH 4 ) titer of 98.5% v , demonstrating the ability of the new strain for upgrading biogas to renewable natural gas. IMPORTANCE As a methanogenic archaeon, Methanothermobacter wolfeii uses CO 2 as electron acceptor, producing CH 4 as final product. The metabolism of M. wolfeii can be harnessed to capture CO 2 from industrial emissions, besides producing a drop-in renewable biofuel to substitute fossil natural gas. If used as biocatalyst in new-generation CO 2 sequestration processes, M. wolfeii has the potential to accelerate the decarbonization of the energy generation sector, which is the biggest contributor of CO 2 emissions worldwide. Nonetheless, the development of CO 2 sequestration archaeal-based biotechnology is still limited by an uncertainty in the requirements to cultivate methanogenic archaea and the unknown longevity of archaeal cultures. In this study, we report the adaptation, isolation, and phenotypic characterization of a novel variant of M. wolfeii , which is capable of maximum growth with minimal nutrients input. Our findings demonstrate the potential of this variant for the production of renewable natural gas, paving the way for the development of more efficient and sustainable CO 2 sequestration processes.

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Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen Methanothermobacter sp. Strain KEPCO-1

Cited by 3 publications

References 12 publications

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Complete Genome Sequence of Methanothermobacter sp. Strain THM-1, a Thermophilic and Hydrogenotrophic Methanogen Isolated from an Anaerobic Reactor in South Korea

Phenotypic and genomic characterization of Methanothermobacter wolfeii strain BSEL, a CO ₂ -capturing archaeon with minimal nutrient requirements

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Complete Genomic Sequence of the Thermophilic and Hydrogenotrophic Methanogen Methanothermobacter sp. Strain KEPCO-1

Cited by 3 publications

References 12 publications

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Complete genome sequence of Methanothermobacter sp. DP, a hydrogenotrophic and thermophilic methanogen

Complete Genome Sequence of Methanothermobacter sp. Strain THM-1, a Thermophilic and Hydrogenotrophic Methanogen Isolated from an Anaerobic Reactor in South Korea

Phenotypic and genomic characterization of Methanothermobacter wolfeii strain BSEL, a CO 2 -capturing archaeon with minimal nutrient requirements

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Phenotypic and genomic characterization of Methanothermobacter wolfeii strain BSEL, a CO ₂ -capturing archaeon with minimal nutrient requirements