Complete Genomic Sequence of the
            <i>O</i>
            -Desmethylangolensin-Producing Bacterium Clostridium rRNA Cluster XIVa Strain SY8519, Isolated from Adult Human Intestine

Yokoyama, Shin-ichiro; Oshima, Koichiro; Nomura, Izumi; Hattori, Masahira; Suzuki, Tomohiko

doi:10.1128/jb.05637-11

Cited by 12 publications

(6 citation statements)

References 7 publications

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“…The metabolite DAGs were the activators of protein kinase C, which could regulate colonic mucosal proliferation [51]. What's more, species of Clostridium are the main force to utilize phenolics, like flavanones, isoflavones, flavonols and flavan-3-ols [52,53]. Most bioactive metabolites from phenolics metabolism are of great benefit to our health.…”

Section: Protein and Other Substances Metabolisms Of Clostridium Speciesmentioning

confidence: 99%

Clostridium species as probiotics: potentials and challenges

Guo

Zhang

et al. 2020

J Animal Sci Biotechnol

307

192

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Clostridium species, as a predominant cluster of commensal bacteria in our gut, exert lots of salutary effects on our intestinal homeostasis. Up to now, Clostridium species have been reported to attenuate inflammation and allergic diseases effectively owing to their distinctive biological activities. Their cellular components and metabolites, like butyrate, secondary bile acids and indolepropionic acid, play a probiotic role primarily through energizing intestinal epithelial cells, strengthening intestinal barrier and interacting with immune system. In turn, our diets and physical state of body can shape unique pattern of Clostridium species in gut. In view of their salutary performances, Clostridium species have a huge potential as probiotics. However, there are still some nonnegligible risks and challenges in approaching application of them. Given this, this review summarized the researches involved in benefits and potential risks of Clostridium species to our health, in order to develop Clostridium species as novel probiotics for human health and animal production.

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Section: Protein and Other Substances Metabolisms Of Clostridium Speciesmentioning

confidence: 99%

Clostridium species as probiotics: potentials and challenges

Guo

Zhang

et al. 2020

J Animal Sci Biotechnol

307

192

View full text Add to dashboard Cite

show abstract

“…The letters G, R, and M in the in silico model represents the number of genes, reactions, and metabolites, respectively. The corresponding reference is indicated at the end of strain's name. For online version, colors indicate as follows: light blue, C. acetobutylicum ; light orange, C. kluyveri ; light purple, C. cellulolyticum ; light green, C. beijerinckii ; light gray, Clostridium sp.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Metabolic engineering of clostridia for the production of chemicals

Cho

Jang

Moon

et al. 2014

Biofuels Bioprod Bioref

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There have recently been signifi cant advances in bio-based production of chemicals from renewable resources. Microorganisms belonging to the genus Clostridium have been considered as one of the promising hosts for the production of desired chemicals of wide industrial use. Clostridium strains have capability to utilize diverse carbon sources, including C5 and C6 substrates, which thus allows production of chemicals from inexpensive and abundant biomass such as corn stover, straw, and woody waste. In addition, Clostridium strains naturally produce various chemicals, such as acetic acid, butyric acid, ethanol, isopropanol, butanol, 1,3-propanediol, 2,3-butanediol, and acetone. Recently, several important strategies for the metabolic engineering of Clostridium have been developed not only for the enhanced production of these natural products and but also for the production of non-natural isobutanol production. Here, we review the strategies employed for the development of metabolically engineered Clostridium strains for the production of such chemicals and provide future perspectives.

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“…Hypothetical gene products sharing lower levels of identity are also found in other flavonoid-degrading bacteria, such as Eubacterium oxidoreducens (SDB03427, 80% identity), Clostridium sp. strain SY8519 (BAK47992, 77% identity), and Flavonifractor plautii (CUQ13575, 42% identity) (10,(25)(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

An NADH-Dependent Reductase from Eubacterium ramulus Catalyzes the Stereospecific Heteroring Cleavage of Flavanones and Flavanonols

Braune

Gütschow

Blaut

2019

Appl Environ Microbiol

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The human intestinal anaerobe Eubacterium ramulus is known for its ability to degrade various dietary flavonoids. In the present study, we demonstrate the cleavage of the heterocyclic C-ring of flavanones and flavanonols by an oxygen-sensitive NADH-dependent reductase, previously described as enoate reductase, from E. ramulus. This flavanone- and flavanonol-cleaving reductase (Fcr) was purified following its heterologous expression in Escherichia coli and further characterized. Fcr cleaved the flavanones naringenin, eriodictyol, liquiritigenin, and homoeriodictyol. Moreover, the flavanonols taxifolin and dihydrokaempferol served as substrates. The catalyzed reactions were stereospecific for the (2R)-enantiomers of the flavanone substrates and for the (2S,3S)-configured flavanonols. The enantioenrichment of the nonconverted stereoisomers allowed for the determination of hitherto unknown flavanone racemization rates. Fcr formed the corresponding dihydrochalcones and hydroxydihydrochalcones in the course of an unusual reductive cleavage of cyclic ether bonds. Fcr did not convert members of other flavonoid subclasses, including flavones, flavonols, and chalcones, the latter indicating that the reaction does not involve a chalcone intermediate. This view is strongly supported by the observed enantiospecificity of Fcr. Cinnamic acids, which are typical substrates of bacterial enoate reductases, were also not reduced by Fcr. Based on the presence of binding motifs for dinucleotide cofactors and a 4Fe-4S cluster in the amino acid sequence of Fcr, a cofactor-mediated hydride transfer from NADH onto C-2 of the respective substrate is proposed. IMPORTANCE Gut bacteria play a crucial role in the metabolism of dietary flavonoids, thereby contributing to their activation or inactivation after ingestion by the human host. Thus, bacterial activities in the intestine may influence the beneficial health effects of these polyphenolic plant compounds. While an increasing number of flavonoid-converting gut bacterial species have been identified, knowledge of the responsible enzymes is still limited. Here, we characterized Fcr as a key enzyme involved in the conversion of flavonoids of several subclasses by Eubacterium ramulus, a prevalent human gut bacterium. Sequence similarity of this enzyme to hypothetical proteins from other flavonoid-degrading intestinal bacteria in databases suggests a more widespread occurrence of this enzyme. Functional characterization of gene products of human intestinal microbiota enables the assignment of metagenomic sequences to specific bacteria and, more importantly, to certain activities, which is a prerequisite for targeted modulation of gut microbial functionality.

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Complete Genomic Sequence of the O -Desmethylangolensin-Producing Bacterium Clostridium rRNA Cluster XIVa Strain SY8519, Isolated from Adult Human Intestine

Abstract: The O-desmethylangolensin-producing Clostridium rRNA cluster XIVa strain SY8519 was isolated from the intestinal flora of a healthy human as a key isoflavonoid-metabolizing bacterium. Here, we report the finished and annotated genomic sequence of this organism.

Cited by 12 publications

References 7 publications

Clostridium species as probiotics: potentials and challenges

Clostridium species as probiotics: potentials and challenges

Metabolic engineering of clostridia for the production of chemicals

An NADH-Dependent Reductase from Eubacterium ramulus Catalyzes the Stereospecific Heteroring Cleavage of Flavanones and Flavanonols

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