2011
DOI: 10.1128/jvi.06402-11
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Complete Genomic Sequence of Bluetongue Virus Serotype 16 from China

Abstract: We report here the complete genomic sequence of the Chinese bluetongue virus serotype 16 (BTV16) strain BN96/16. This work is the first to document the complete genomic sequence (segments 1 to 10) of a BTV16 strain. The sequence information provided herein will help determine the geographic origin of BTV16 and define the phylogenetic relationship of BTV16 to other BTV strains.Bluetongue virus (BTV) is the "type" species of the genus Orbivirus, within the family Reoviridae (7). It is the etiological agent of bl… Show more

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Cited by 15 publications
(14 citation statements)
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“…The sequences were assembled with the Newbler assembler (454 Life Sciences). The GS reference mapper was used to generate contigs (with BTV16 from China used as a reference genome (13)), and the resulting contigs were used for aligning it to the reference sequence (13), which generated a consensus sequence of sample cDNA libraries. The gaps that remained at the ends were filled by Sanger's sequencing.…”
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confidence: 99%
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“…The sequences were assembled with the Newbler assembler (454 Life Sciences). The GS reference mapper was used to generate contigs (with BTV16 from China used as a reference genome (13)), and the resulting contigs were used for aligning it to the reference sequence (13), which generated a consensus sequence of sample cDNA libraries. The gaps that remained at the ends were filled by Sanger's sequencing.…”
mentioning
confidence: 99%
“…The gaps that remained at the ends were filled by Sanger's sequencing. The genome annotation and comparative analysis of the genome were carried out with published genome of BTV16 from China (13).…”
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confidence: 99%
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“…Previously, two BTV-16 field strains from China and Australia have been fully sequenced (3,16). All genome segments of the BTV-16 reference strain (RSArrrr/16) showed Ͼ99% sequence identity to the BTV-16 vaccine strain (RSAvvvv/16) that was derived from it and to the Chinese BTV-16 (strain BN96/16) isolated from a sheep in Yunnan province during 1996 (16).…”
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confidence: 99%
“…Viral double-stranded RNA (dsRNA) preparation, full-length cDNA synthesis, PCR amplification, cloning, and sequencing of SZ97/1 were performed according to the protocol described by Yang et al (11).…”
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confidence: 99%