Complete genomic sequence of bacteriophage P23: a novel Vibrio phage isolated from the Yellow Sea, China

Liu, Huan; Zhao, Lei; Wang, Min; Wang, Qi; Zhang, Xinran; Han, Yuye; Wang, Meiwen; Jiang, Yong; Shao, Hongbing; McMinn, Andrew

doi:10.1007/s11262-019-01699-3

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…Phages are vital for renewal of organic matters, provisions of nutrient supply cycles, serve as genomic reservoirs and drive the bacterial diversity. Several studies has investigated the occurrence of bacteriophages in various water sources [ 2 , 26 , 27 ]. Moreover, bacteriophages detection has been considered as important indicator for fecal pollution [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

et al. 2022

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The diversity among bacteriophages depends on different factors like ecology, temperature conditions and genetic pool. Current study focused on isolation, identification and diversity of phages from 34 sewage water samples collected from two different wastewater treatment plants (WWTPs), King Saud University wastewater treatment plants (KSU-WWTP) and Manfoha wastewater treatment plants (MN-WWTP) in Riyadh, Saudi Arabia. Samples were analyzed by PCR and Next Generation Sequencing (NGS). Siphoviridae, Podoviridae and Myoviridae families were detected by family-specific PCR and highest prevalence of Myoviridae 29.40% was found at MN-WWTP followed by 11.76% at KSU-WWTP. Siphoviridae was detected 11.76% at MN-WWTP and 5.88% at KSU-WWTP. Lowest prevalence for Podoviridae family (5.88%) was recorded at MN-WWTP. Significant influence of temporal variations on prevalence of Myoviridae and Siphoviridae was detected in both WWTP and MN-WWTP, respectively. Highest phage prevalence was obtained in August (75%), followed by September (50%). Highest phage prevalence was recorded at a temperature range of 29–33°C. Significant influence of temperature on the prevalence of Myoviridae phages was detected at MN-WWTP. Four bacteriophages with various abundance levels were identified by NGS. Cronobacter virus Esp2949-1 was found first time with highest abundance (4.41%) in wastewater of Riyadh. Bordetella virus BPP1 (4.14%), Dickeya virus Limestone (1.55%) and Ralstonia virus RSA1 (1.04%) were also detected from samples of MN-WWTP. Highest occurrence of Bordetella virus BPP1 (67%) and (33.33%) was recorded at KSU-WWTP and MN-WWTP, respectively. Highest Bordetella virus BPP1 occurrence was recorded in September (50%) followed by August (40%). The findings of study showed new insights of phage diversity from wastewater sources and further large-scale data studies are suggested for comprehensive understanding.

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Section: Discussionmentioning

confidence: 99%

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

et al. 2022

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“…The morphological and genomic characteristics indicated that phage vB_VnaS-L3 was affiliated with Siphoviridae . It had the closest phylogenetic relationships with Listonella phage φHSIC [ 78 ], Vibrio phage ValSw3-3 [ 19 ], Vibrio phage pYD38-B, and Vibrio phage P23 [ 79 ], all of which may belong to the same viral genus judged from their pairwise OrthoANI values ( Figure 6 D) [ 80 ]. Functional differences could be found between phage vB_VnaS-L3 and phage φHSIC, based on the genomic comparison, implying that they may have different survival strategies in their respective bacterial hosts ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

“…Vibrio phage OMN was observed to tolerate changes in pH (5–9) and temperature (up to 50 °C) [ 85 ]. Moreover, phage vB_VnaS-L3 had a large burst size (~890 PFU/cell), which was about 10–40 times larger than other evolutionarily related phages and potential phage candidates for vibriosis therapy, showing a more effective pathogen lytic effect [ 19 , 79 , 85 , 86 , 87 ]. In addition, the killing curve showed that phage vB_VnaS-L3 is effective at reducing V. natriegens population at different MOI.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Vibrio natriegens—Infecting Phage and Its Potential Therapeutic Application in Abalone Aquaculture

Liang

Wang

et al. 2022

Biology

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Phage-based pathogen control (i.e., phage therapy) has received increasing scientific attention to reduce and prevent the emergence, transmission, and detrimental effects of antibiotic resistance. In the current study, multidrug-resistant Vibrio natriegens strain AbY-1805 was isolated and tentatively identified as a pathogen causing the death of juvenile Pacific abalones (Haliotis discus hannai Ino). In order to apply phage therapy, instead of antibiotics, to treat and control V. natriegens infections in marine aquaculture environments, a lytic phage, vB_VnaS-L3, was isolated. It could effectively infect V. natriegens AbY-1805 with a short latent period (40 min) and high burst size (~890 PFU/cell). Treatment with vB_VnaS-L3 significantly reduced the mortality of juvenile abalones and maintained abalone feeding capacity over a 40-day V. natriegens challenge experiment. Comparative genomic and phylogenetic analyses suggested that vB_VnaS-L3 was a novel marine Siphoviridae-family phage. Furthermore, vB_VnaS-L3 had a narrow host range, possibly specific to the pathogenic V. natriegens strains. It also exhibited viability at a wide range of pH, temperature, and salinity. The short latent period, large burst size, high host specificity, and broad environmental adaptation suggest that phage vB_VnaS-L3 could potentially be developed as an alternative antimicrobial for the control and prevention of marine animal infections caused by pathogenic V. natriegens.

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“…7H 2 O, 50 mM Tris–HCl (pH 7.5), 0.01% gelatin]. Purified bacteriophage was stored in SM buffer at 4°C for a few months for further processing ( Liu et al, 2019 ). The host bacterial strain M. algicola DG893(T) was incubated in LB liquid medium at 28°C.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria

et al. 2021

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Marinobacter is the abundant and important algal-associated and hydrocarbon biodegradation bacteria in the ocean. However, little knowledge about their phages has been reported. Here, a novel siphovirus, vB_MalS-PS3, infecting Marinobacter algicola DG893(T), was isolated from the surface waters of the western Pacific Ocean. Transmission electron microscopy (TEM) indicated that vB_MalS-PS3 has the morphology of siphoviruses. VB_MalS-PS3 was stable from −20 to 55°C, and with the latent and rise periods of about 80 and 10 min, respectively. The genome sequence of VB_MalS-PS3 contains a linear, double-strand 42,168-bp DNA molecule with a G + C content of 56.23% and 54 putative open reading frames (ORFs). Nineteen conserved domains were predicted by BLASTp in NCBI. We found that vB_MalS-PS3 represent an understudied viral group with only one known isolate. The phylogenetic tree based on the amino acid sequences of whole genomes revealed that vB_MalS-PS3 has a distant evolutionary relationship with other siphoviruses, and can be grouped into a novel viral genus cluster with six uncultured assembled viral genomes from metagenomics, named here as Marinovirus. This study of the Marinobacter phage vB_MalS-PS3 genome enriched the genetic database of marine bacteriophages, in addition, will provide useful information for further research on the interaction between Marinobacter phages and their hosts, and their relationship with algal blooms and hydrocarbon biodegradation in the ocean.

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Complete genomic sequence of bacteriophage P23: a novel Vibrio phage isolated from the Yellow Sea, China

Cited by 16 publications

References 39 publications

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

Novel findings in context of molecular diversity and abundance of bacteriophages in wastewater environments of Riyadh, Saudi Arabia

Isolation and Characterization of a Novel Vibrio natriegens—Infecting Phage and Its Potential Therapeutic Application in Abalone Aquaculture

Characterization and Genomic Analysis of Marinobacter Phage vB_MalS-PS3, Representing a New Lambda-Like Temperate Siphoviral Genus Infecting Algae-Associated Bacteria

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