Complete genomic characterization of cell culture adapted human G12P[6] rotaviruses isolated from South Korea

Than, Van Thai; Le, Van Phan; Lim, Inseok; Kim, Wonyong

doi:10.1007/s11262-011-0576-x

Cited by 10 publications

(2 citation statements)

References 48 publications

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“…The GCRV dsRNA was extracted from a fecal sample by using Trizol (Gibco BRL, Grand Island, NY) according to the manufacturer's instructions. To obtain full‐length sequences of the 11 gene segments, the primers were obtained from the study of Yamamoto et al [], and the RT‐PCR reaction was carried out as previously described [Than et al, ].…”

Section: Methodsmentioning

confidence: 99%

Full genomic characterization of a group C rotavirus isolated from a child in south Korea

Baek

Than

Kim

et al. 2013

Journal of Medical Virology

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During an epidemiological survey of human rotavirus infection in Seoul, Korea, from 2010 to 2011, one isolate of group C rotavirus (GCRV), named CAU10-312, was detected in a 5-year-old child admitted to the hospital with acute gastroenteritis, and its complete genomic sequence was determined. The 11 gene segments of the strain possessed G4-P[2]-I2-R2-C2-M2-A2-N2-T2-E2-H2 genotypes. The genotype of strain CAU10-312 appears to be closely related to strains from Bangladesh (DhakaC13 and BS347), India (v508), and England (Bristol), but distinct from Far East Asian strains, Chinese (Wu82 and YNR001) and Japanese (OH567 and BK0830). These findings may clarify the relationship of the genetics, evolutionary biology, and epidemiology of GCRVs and suggest that two very distinct genotype strains are in circulation in the world.

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Section: Methodsmentioning

confidence: 99%

Full genomic characterization of a group C rotavirus isolated from a child in south Korea

Baek

Than

Kim

et al. 2013

Journal of Medical Virology

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“…2A ) 54 , 63) . Phylogenetic analysis of all 11 gene sequences revealed that these genotypes belonged to genogroup I of the Wa-like genotype constellation 64) . These results suggest that G12 genotypes not only are persistent but also may have adapted and evolved in the South Korean population.…”

Section: Clinical Significance Of Unusual G Genotypes In South Koreamentioning

confidence: 99%

Prevalence of rotavirus genotypes in South Korea in 1989-2009: implications for a nationwide rotavirus vaccine program

Than

Kim

2013

Korean J Pediatr

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The epidemiology of human group A rotavirus was analyzed by examining genotypic data acquired from 1989 to 2009 in South Korea. This information was derived from all the available published articles on rotavirus studies in South Korea, retrieved from both the PubMed and KoreaMed databases. Four common G types (G1, G2, G3, and G4) and three common P types (P[8], P[4], and P[6]) accounted for approximately 93% and 99% of the rotavirus reports, respectively. The G9 type was frequently detected after 2000, and because of this prevalence, it is considered to be the fifth most important G type rotavirus after the G1.G4 genotypes. Less common G types of the virus such as G12, G11, and G10 were detected in some geographic settings, and it is important to consider the context of these subtypes and their epidemiological significance. The P[9] virus genotype was observed in the study and has been discussed in many other studies; however, the P[3], P[10] and P[25] genotypes were rarely detected in the epidemiological research. In general, the distributions of the G and P genotypes showed temporal and geographical fluctuations, and a nationwide rotavirus vaccine program that targeted these genotypes demonstrated effectiveness in protecting against the circulating rotavirus strains. However, further analysis is needed to determine the true long-term effectiveness of these vaccines; the analysis should also consider the unexpected effects of vaccinations, such as vaccine-induced diseases, herd immunity, and changes in host susceptibilities.

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Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG)

Ciarlet²,

et al. 2011

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In April 2008, a nucleotide sequence-based, complete genome classification system was developed for group A rotaviruses (RVs). This system assigns a specific genotype to each of the 11 genome segments of a particular RV strain according to established nucleotide percent cut-off values. Using this approach, the genome of individual RV strains are given the complete descriptor of Gx-P[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx. A Rotavirus Classification Working Group (RCWG) was formed by scientists in the field to maintain, evaluate, and develop the RV genotype classification system, in particular to aid in the designation of new genotypes. Since its conception, the group has ratified 50 new genotypes: as of January 2011, new genotypes for VP7 (G20–G26), VP4 (P[28]–P[35]), VP6 (I12–I16), VP1 (R5–R9), VP2 (C6–C9), VP3 (M7–M8), NSP1 (A15–A16), NSP2 (N6–N9), NSP3 (T8–T12), NSP4 (E12–E14), and NSP5/6 (H7–H11) have been defined for RV strains identified in humans, cows, pigs, horses, mice, South American camelids (guanaco and vicuña), chickens, turkeys, pheasants, and bats. With increasing numbers of complete RV genome sequences becoming available, a standardized RV strain nomenclature system is needed and the RCWG proposes that individual RV strains are named as follows: RV group/species of origin/country of identification/common name/year of identification/G- and P-type. In collaboration with the National Center for Biotechnology Information (NCBI), the RCWG is also working on developing a RV-specific resource for the deposition of nucleotide sequences. This resource will provide useful information regarding RV strains, including but not limited to, the individual gene genotypes, epidemiological, and clinical information. Together, the proposed nomenclature system and the NCBI RV resource will offer highly useful tools for investigators to search for, retrieve, and analyze the ever-growing volume of RV genomic data.

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Complete genomic characterization of cell culture adapted human G12P[6] rotaviruses isolated from South Korea

Cited by 10 publications

References 48 publications

Full genomic characterization of a group C rotavirus isolated from a child in south Korea

Full genomic characterization of a group C rotavirus isolated from a child in south Korea

Prevalence of rotavirus genotypes in South Korea in 1989-2009: implications for a nationwide rotavirus vaccine program

Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG)

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