Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant

Dedepsidis, Evaggelos; Pliaka, Vaia; Kyriakopoulou, Zaharoula; Brakoulias, Christos; Levidiotou-Stefanou, Stamatina; Pratti, Anastassia; Mamuris, Zissis; Markoulatos, Panayotis

doi:10.1111/j.1574-695x.2008.00381.x

Cited by 31 publications

(23 citation statements)

References 42 publications

(58 reference statements)

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“…Alternatively, these discrepancies may have resulted from recombination events that had occurred within the capsid region. Such events are believed to be infrequent, probably because of the structural constraints imposed on virions, but previous studies have shown that they can occur in both PV species (14,15,23,36,39,60,67) and non-PV HEV species (16). Further analyses are under way to determine whether the discrepancies observed between the VP1 and VP2 methods for some samples can be attributed to such uncommon recombination events.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Human Enteroviruses in the Central African Republic: Uncovering Wide Diversity and Identification of a New Human Enterovirus A71 Genogroup

et al. 2012

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dHuman enteroviruses (HEV) are among the most common viruses infecting humans. Their circulation has been widely studied in most parts of the world but not in sub-Saharan Africa, where poliomyelitis remains prevalent. We report here the molecular characterization of 98 nonpoliovirus (non-PV) HEV strains isolated from 93 randomly selected cell culture-positive supernatants from stool samples collected from 1997 through 2006 from children with acute flaccid paralysis living in the Central African Republic (CAR). The isolates were typed by sequencing the VP1 coding region and sequenced further in the VP2 coding region, and phylogenetic studies were carried out. Among the 98 VP1 sequences, 3, 74, 18, and 3 were found to belong to the HEV-A, -B, -C, and -D species, respectively. Overall, 42 types were detected. In most cases, the VP2 type was correlated with that of the VP1 region. Some of the isolates belonged to lineages that also contain viruses isolated in distant countries, while others belonged to lineages containing viruses isolated only in Africa. In particular, one isolate (type EV-A71) did not fall into any of the genogroups already described, indicating the existence of a previously unknown genogroup for this type. These results illustrate the considerable diversity of HEV isolates from the stools of paralyzed children in the CAR. The presence of diverse HEV-C types makes recombination between poliovirus and other HEV-C species possible and could promote the emergence of recombinant vaccine-derived polioviruses similar to those that have been implicated in repeated poliomyelitis outbreaks in several developing countries.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Human Enteroviruses in the Central African Republic: Uncovering Wide Diversity and Identification of a New Human Enterovirus A71 Genogroup

et al. 2012

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“…The enterovirus recombination sites are located in the 59 non-coding region (NCR) and in the non-structural part of the 7500 nt genome in genes coding for proteins 2A, 2B, 2C and 3D. In addition, recombination in the capsid protein VP1 coding region has been shown Dedepsidis et al, 2008;Liu et al, 2000;Martin et al, 2002), as well as recombination in the VP2 protein coding gene (Kyriakopoulou et al, 2006). In addition to within-species recombination events, interspecies recombination has been suggested for HEV-B and HEV-C strains (Bolanaki et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparison of poliovirus recombinants: accumulation of point mutations provides further advantages

Savolainen-Kopra

Самойлович

Kahelin

et al. 2009

Journal of General Virology

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The roles of recombination and accumulation of point mutations in the origin of new poliovirus (PV) characteristics have been hypothesized, but it is not known which are essential to evolution. We studied phenotypic differences between recombinant PV strains isolated from successive stool specimens of an oral PV vaccine recipient. The studied strains included three PV2/PV1 recombinants with increasing numbers of mutations in the VP1 gene, two of the three with an amino acid change IAT in the DE-loop of VP1, their putative PV1 parent and strains Sabin 1 and 2. Growth of these viruses was examined in three cell lines: colorectal adenocarcinoma, neuroblastoma and HeLa. The main observation was a higher growth rate between 4 and 6 h post-infection of the two recombinants with the IAT substitution. All recombinants grew at a higher rate than parental strains in the exponential phase of the replication cycle. In a temperature sensitivity test, the IATsubstituted recombinants replicated equally well at an elevated temperature. Complete genome sequencing of the three recombinants revealed 12 (3), 19 (3) and 27 (3) nucleotide (amino acid) differences from Sabin. Mutations were located in regions defining attenuation, temperature sensitivity, antigenicity and the cis-acting replicating element. The recombination site was in the 59 end of 3D. In a competition assay, the most mutated recombinant beat parental Sabin in all three cell lines, strongly suggesting that this virus has an advantage. Two independent intertypic recombinants, PV3/PV1 and PV3/PV2, also showed similar growth advantages, but they also contained several point mutations. Thus, our data defend the hypothesis that accumulation of certain advantageous mutations plays a key role in gaining increased fitness.

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“…In the last few years, more attention has been shifted toward nonpoliovirus enteroviruses, since it has been proven that many prototype strains, as well as Echo18 and CAV21 strains (1,20,35,36) are recombinants and there is more and more evidence for genetic exchange between circulating enteroviruses (30,35). Even though much progress has been made in studies of genetic exchange between enteroviruses, recombination junctions have been detected only in recombinant Sabin poliovirus strains (13,22).…”

Section: Discussionmentioning

confidence: 99%

Full-Genome Sequence Analysis of a Multirecombinant Echovirus 3 Strain Isolated from Sewage in Greece

et al. 2010

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An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region revealed that the isolated strain correlates with Echo3 strains isolated during the same year in France and Japan, implying that the same virus circulated in Europe and Asia. LR31G7 was found to be a recombinant that shares the 3 part of its genome with an Echo25 strain isolated from asymptomatic infants in Norway in 2003. Nucleotide and SimPlot analyses of the VP1-2A junction, where the recombination was located, revealed the exact recombination breakpoint (nucleotides 3357 to 3364). Moreover, there is evidence that recombination events had occurred in 3B-3D region in the evolutionary history of the isolate. Our study indicates that recombination events play major roles in enterovirus evolution and that the circulation of multirecombinant strains with unknown properties could be potentially dangerous for public health.

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Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant

Cited by 31 publications

References 42 publications

Molecular Characterization of Human Enteroviruses in the Central African Republic: Uncovering Wide Diversity and Identification of a New Human Enterovirus A71 Genogroup

Molecular Characterization of Human Enteroviruses in the Central African Republic: Uncovering Wide Diversity and Identification of a New Human Enterovirus A71 Genogroup

Comparison of poliovirus recombinants: accumulation of point mutations provides further advantages

Full-Genome Sequence Analysis of a Multirecombinant Echovirus 3 Strain Isolated from Sewage in Greece

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