Complete genomic characterisation of two novel poxviruses (WKPV and EKPV) from western and eastern grey kangaroos

RETRACTED ARTICLE: Molecular and microscopic characterization of a novel Eastern grey kangaroopox virus genome directly from a clinical sample

“…4A ). However, EKPV-NSW generated a sister clade along with the recently isolated EKPV-SC and WKPV-WA 16 . The DNA polymerase gene orthologs of EKPV-NSW showed amino acid identities ranging from 35 to 67.72%, to other chordopoxviruses (Fig.…”

“…Pox lesions have been recognised in kangaroos for at least 3 decades with cases reported in a female eastern grey kangaroo held in captivity on a property in Nerang, Queensland, along with molluscum contagiosum in a red kangaroo in the 1970s. With the exception of very recently isolated KPVs 16 , poxvirus-like particles were confirmed to be present in the lesions elecronmicroscopically 14 , 15 , however, no taxonomic classification has been granted yet by the ICTV and until now, unresolved relationships with other members of the family Poxviridae . In this study, we confirmed the infection of grey kangaroo with a novel species of poxvirus, and determined the complete genome sequence with predicted full-length coding regions including ITRs, and nominate this as the type genome for Eastern grey kangaroopox virus strain NSW (EKPV-NSW).…”

“…The genome of EKPV-NSW is sufficiently diverse from recently isolated KPVs 16 , as well as all other known poxvirus genomes sequenced to date, and we propose for it to be classified within a new poxvirus genus Marsupialiapoxvirus . The observed sequence similarity of EKPV-NSW with other poxviruses is expected, given the extensive genetic diversity, and phylogeographic separation of KPVs from other Poxviridae 28 .…”

“…Eastern grey kangaroopox virus (EKPV) has been nominated as a member of the subfamily Chordopoxvirinae , based on histopathological lesion, and electron microscopy 14 , 15 , as has the recently described novel Eastern grey kangaroopox virus 16 , but the ICTV has not yet approved it as a species. In Australia, kangaroo pox has been recognised in a wide range of native macropod species 14 , 15 , 17 including the eastern ( Macropus giganteus ) and western grey kangaroo ( Macropus fuliginosus ) species, red kangaroo ( Macropus rufus ), common wallaroo ( Macropus robustus ), tammar wallaby ( Macropus eugenii ), agile wallaby ( Macropus agilis ), swamp wallaby ( Wallabia bicolor ), Tasmanian pademelon ( Thylogale billardierii ) and quokka ( Setonix brachyurus ) 18 .…”

Recombination drives the emergence of orf virus diversity: evidence from the first complete genome sequence of an Indian orf virus isolate and comparative genomic analysis

“…Thus, China/GO/2012 was used as a reference genome for mapping and mutation analysis. Viral DNA was isolated from a clinical sample, and sequenced using a NextSeq 500 NGS platform [ 7 , 8 ]. The resulting genome sequence, with a total length of 139,807 bp, was submitted to the GenBank database under accession number MT332357.…”